LOCUS       sequence-372018-        2861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  template for co-transcriptional RNA folding assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Huang YH, Hilal T, Loll B, Burger J, Mielke T, Bottcher C, Said N, 
            Wahl MC
  TITLE     Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone 
            Machine Required for Ribosome Biosynthesis.
  JOURNAL   Mol Cell. 2020 Sep 17;79(6):1024-1036.e5. doi: 
            10.1016/j.molcel.2020.08.010. Epub 2020 Aug 31.
  PUBMED    32871103
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.08.010"; journalName: "Mol Cell"; date: 
            "2020-09-17- 17"; volume: "79"; issue: "6"; pages: "1024-1036.e5"
COMMENT     Sequence Label: pUC18-iSpinach
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(59..81)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     181..200
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     394..416
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     408..425
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     409..425
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(683..705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    694..710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(718..724,725..742,743..748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    763..784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(901..918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1072..1660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1152..1171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1831..2622,2623..2691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2454..2473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2692..2796)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
       61 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
      121 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      181 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      241 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
      301 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      361 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      421 ccagtgccaa gcttaatgac ttaaagtcta acctatagta taattacagc catcgagagg
      481 gacacgggga aacaccacca cgccgctgag aaaaagcgaa gcggcactgc tctttaacaa
      541 tttatcagac aatctgtgtg ggcgggagta cggtgagggt cgggtccagt aggtacgcct
      601 actgtgtagt agagtgtggg ctccgtactc cccaaattat gcgggtaccg agctcgaatt
      661 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2821 tgacgtctaa gaaaccatta ttatcatgac attaacctat a
//
