LOCUS       sequence-372117-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-12 ONE-GO
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1072..1091)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1231..1482
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1419..1440
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2237..2811,2812..2814,2815..2823)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2404..2421)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2631..2650
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2834..2854
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3503..4018
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4032..4620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4085..4105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4503..4514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4827..5460
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5588..5604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5588..5604)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5601..5623)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5612..5628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5636..5642,5643..5660,5661..5666))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5681..5702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5819..5836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6070..6089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6749..7540,7541..7609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7372..7391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7610..7714)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7762..7896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7799..7818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7853..7872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8064..8697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8744..8869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9366..9599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9784..9828
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10091..10206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10264..10567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgt caggtgtagt aaggacattg
      361 tcccgatgtc tgttgcccgc agaggcgggg ggcgctcgcg aaagacgcgc gggctctggc
      421 gcccgggacg ctgaaaggga agcgcgaaga agatcacgcg atattgacgc gcttcttgcg
      481 cgggaaagga gagcggtccg cagactcgtg aaaatattgc tgctcggggc tggcgagtct
      541 ggcaaatcca cgttcctcaa acaaatgaga atcatacacg ggcgggaatt cgaccaaaaa
      601 gctttgctcg aattcaggga cactatcttt gataatatac tcaaagggtc ccgggttctc
      661 gttgatgcca gggacaagct cggcattccg tggcaatact ctgagaatga aaagcatggc
      721 atgtttttga tggctttcga gaacaaagcg ggacggggat caggcatggt ttcaaaggga
      781 gaagagttgt tcacaggggt tgtaccgatt cttgtagaac ttgacggaga tgtcaacggg
      841 cacaagttta gtgtaagtgg agaaggcgag ggcgatgcga cctatggaaa actgaccctt
      901 aaatttattt gtactacggg taaattgccc gttccctggc cgacattggt aactacattc
      961 ggatatggtt tgatgtgctt tgcaaggtat ccggatcaca tgaagcagca tgatttcttc
     1021 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1081 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1141 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1201 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1261 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1321 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1381 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1441 accgccgccg ggatcactct cggcatggac gagctgtaca agggatcagg cccgcggctt
     1501 ccagtggaac cagcaacgtt tcagctttat gtgcctgctc ttagcgctct ctggagagat
     1561 tcagggatcc gagaggcgtt ttcacgacgg tccgaattcc aactgggaga atccgtgaaa
     1621 tattttctgg acaatctcga tcgcatcggt caattgaatt atttccccag caagcaagat
     1681 atccttctgg ctcgcaaggc gacgaaaggt atagtagagc atgacttcgt cataaaaaaa
     1741 atccctttta aaatggttga tgtaggaggc cagaggtcac agcggcaaaa atggtttcaa
     1801 tgcttcgacg gaatcacttc tatcttgttc atggtgtcca gttctgaata cgaccaagtg
     1861 ctcatggagg accggaggac caatcgactg gtcgaaagca tgaacatatt cgagactata
     1921 gtgaataaca agttgttctt caatgtaagt attatccttt ttctcaataa aatggatctt
     1981 ttggtagaga aggttaaaac cgtatcaatt aaaaagcatt ttcccgattt tagaggtgac
     2041 ccccaccaac tggaagacgt acagcgatat ctggtccagt gtttcgaccg gaaacgccgc
     2101 aataggtcca agcccctgtt tcaccatttt acgactgcga ttgataccga aaacgttagg
     2161 tttgttttcc atgctgtgaa agatacaata cttcaagaga acctcaagga cattatgctc
     2221 caatgagcgg ccgccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     2281 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     2341 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     2401 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2461 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     2521 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     2581 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     2641 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     2701 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     2761 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca
     2821 acccgtacca aagatgggga gtagcaagag caagacgtcg aattccggaa tcaagcttct
     2881 gattattggc ccagaggaag attatgaccc aggttatttc aacaatgaga gtgacatcat
     2941 cttccaagat cttgaaaaac tgaagtcaca tccagcttac ttggtagttt ttctacgtta
     3001 catcctctct caggcagacc ctggccccct gcttttttat ttgtgttcag aagtttatca
     3061 acagacaaat cccaaagatt cccgaagtct ggggaaagac atctggaaca ttttcctgga
     3121 gaaaaatgcg cctctcagag tgaagatccc tgagatgttg caggctgaaa ttgacctacg
     3181 cctgcggaac aatgaggacc ctcgcaatgt gctctgtgaa gctcaggagg cagtcatgct
     3241 ggaaatccag gagcagatca acgactacag atccaagcgt actctgggcc tgggcagcct
     3301 ctatggtgaa aatgacctgc taggcctgga tggggaccct cttcgagaac gccaaatggc
     3361 tgagaagcag ctggctgccc ttggagatat cttgtccaaa tatgaggaag atcggagtgc
     3421 ccccatggac tttgctgtta atacctttat gagccacgct gggatccgtc ttcgggagtc
     3481 tcgaggcgga tcaggcggat ccatggtctt cacactcgaa gatttcgttg gggactggcg
     3541 acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt
     3601 tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg
     3661 gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg
     3721 ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat
     3781 cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg
     3841 acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct
     3901 gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt
     3961 ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtaaac
     4021 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4081 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4141 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4201 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4261 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4321 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4381 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4441 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4501 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4561 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4621 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     4681 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     4741 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     4801 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     4861 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     4921 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     4981 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     5041 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     5101 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     5161 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     5221 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     5281 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5341 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5401 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5461 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5521 agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt
     5581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7681 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7741 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     7801 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7861 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     7921 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     7981 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8041 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     8101 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     8161 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8221 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     8281 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     8341 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     8401 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     8461 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     8521 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8581 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8641 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8701 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8761 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8821 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8881 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8941 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9001 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9061 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9121 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9181 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     9241 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9301 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9361 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9421 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9481 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9541 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9601 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9661 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9721 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9781 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9841 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9901 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9961 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10021 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    10081 cggtatcgcc tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10141 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10201 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt
    10261 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    10321 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    10381 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    10441 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    10501 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    10561 taccatg
//
