LOCUS       sequence-372199-        1941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expresses human talin R7R8 domain flanked between a HaloTag 
            and a SpyTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Dahal N, Sharma S, Phan B, Eis A, Popa I
  TITLE     Mechanical regulation of talin through binding and history-dependent
            unfolding.
  JOURNAL   Sci Adv. 2022 Jul 15;8(28):eabl7719. doi: 10.1126/sciadv.abl7719. 
            Epub 2022 Jul 15.
  PUBMED    35857491
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-07-15"; volume: "8(28):eabl7719. doi"; pages: " 
            10.1126/sciadv.abl7719. Epub 2022 Jul 15"
COMMENT     Sequence Label: pFN-HaloTag-TalinR7R8-SpyTag
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..891
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             904..924
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1864..1902
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             1918..1935
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
       61 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      121 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      181 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      241 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
      301 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
      361 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
      421 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
      481 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
      541 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
      601 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
      661 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
      721 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
      781 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
      841 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg cgagccaacc
      901 actgaggatc tgtactttca gagcgataac gcgatcgccg gatccatgca gcaggcaccg
      961 ggccaaaaag aatgtgacaa tgccctgcgc gaactggaga ccgtgcgcga actgctggag
     1021 aatccggtgc agccgatcaa tgatatgagt tatttcggct gcctggacag tgtgatggag
     1081 aacagcaaag tgctgggtga ggccatgacc ggtatcagcc agaacgccaa aaacggcaat
     1141 ctgccggaat tcggcgatgc catcagcaca gcaagcaaag ccctgtgcgg ctttaccgaa
     1201 gcagccgccc aggcagcata tctggtgggt gtgagcgatc cgaatagcca ggccggtcaa
     1261 cagggtctgg ttgaaccgac ccagtttgcc cgcgccaatc aggccatcca gatggcatgc
     1321 cagagcttag gtgagccggg ttgcacccag gcccaggttc tgagcgccgc aaccattgtt
     1381 gccaaacata ccagcgccct gtgtaatagc tgccgcctgg caagcgcccg taccacaaat
     1441 ccgaccgcca aacgccagtt cgtgcagagc gccaaagaag tggccaatag cacagccaac
     1501 ctggttaaaa ccatcaaggc cctggacggt gcatttaccg aggaaaatcg cgcacagtgt
     1561 cgcgcagcaa cagccccgct gctggaagca gttgacaacc tgagcgcctt cgccagcaat
     1621 cctgagttta gcagcattcc ggcacagatt agcccggaag gccgcgcagc aatggagccg
     1681 attgtgatca gcgccaaaac aatgctggaa agcgccggcg gcttaatcca gacagcccgt
     1741 gccctggccg tgaatcctcg cgatccgccg agctggagtg ttctggccgg tcatagccgt
     1801 accgtgagcg acagcatcaa gaagctgatc acaagcatgc gcgataaagc accgggtaga
     1861 tccgcccaca tcgtgatggt ggacgcctac aagccgacga aggggggttg tagatcccat
     1921 catcatcatc accactgcta a
//
