LOCUS       sequence-37230-e        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of green fluorescent genetically encoded Ca2+ 
            indicator for optical imaging (version 1.2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-G-GECO1.2
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             814..1257
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct cactcgagaa cgtctatatc aaggccgacg agcagaagaa cggcatcaag
      121 gcgtacttca agatccgcca caacatcgag ggcggcggcg tgcagctcgc ctaccactac
      181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 gtgcagtcca tgctttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
      361 gggagcgaat ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatccag
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag
      661 cgcaccatct tcttcaaggg cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaacacg cgtgaccaac tgactgaaga gcagatcgca
      841 gaatttaaag aggctttctc cctatttgac aaggacgggg atgggacgat aacaaccaag
      901 gagctgggga cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac
      961 atgatcaatg aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca
     1021 atgatggcaa gaaaaatgaa agacacagac agtgaagaag aaattagaga agcgttccgt
     1081 gtgtttgata aggacggcaa tggctacatc ggcgcagcag agcttcgcca cgtgatgaca
     1141 aaccttggag agaagttaac agatgaagag gttgatgaaa tgatcagggt agcagacatc
     1201 gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtag
//
