LOCUS       sequence-372412-        9000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human TYRO3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_TYRO3
FEATURES             Location/Qualifiers
     source          1..9000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..81)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2034..2053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2225..2604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2605..2808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2759..2779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2810..2828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2831..2849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2859..2883
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3026..3050
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5727..5751)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5798..5821
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5858..5881
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5912..5938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5912..5938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5939..6286,6287..6289,6290..6898)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6942..6959)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6948..7172
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7305..7324)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7456..7503
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7511..8533
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7511..8533
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8663..8784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8700..8719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8754..8773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8833..8849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8833..8849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8846..8868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8857..8873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8881..8887,8888..8905,8906..8911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8926..8947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
       61 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     2041 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     2101 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     2161 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     2221 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     2281 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2341 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2401 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2461 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2521 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2581 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2641 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2701 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2761 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
     2821 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
     2881 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     2941 agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
     3001 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatggc
     3061 gctgaggcgg agcatggggc ggccggggct cccgccgctg ccgctgccgc cgccaccgcg
     3121 gctcgggctg ctgctggcgg ctctggcttc tctgctgctc ccggagtccg ccgccgcagg
     3181 tctgaagctc atgggagccc cggtgaagct gacagtgtct caggggcagc cggtgaagct
     3241 caactgcagt gtggagggga tggaggagcc tgacatccag tgggtgaagg atggggctgt
     3301 ggtccagaac ttggaccagt tgtacatccc agtcagcgag cagcactgga tcggcttcct
     3361 cagcctgaag tcagtggagc gctctgacgc cggccggtac tggtgccagg tggaggatgg
     3421 gggtgaaacc gagatctccc agccagtgtg gctcacggta gaaggtgtgc catttttcac
     3481 agtggagcca aaagatctgg cagtgccacc caatgcccct ttccaactgt cttgtgaggc
     3541 tgtgggtccc cctgaacctg ttaccattgt ctggtggaga ggaactacga agatcggggg
     3601 acccgctccc tctccatctg ttttaaatgt aacaggggtg acccagagca ccatgttttc
     3661 ctgtgaagct cacaacctaa aaggcctggc ctcttctcgc acagccactg ttcaccttca
     3721 agcactgcct gcagccccct tcaacatcac cgtgacaaag ctttccagca gcaacgctag
     3781 tgtggcctgg atgccaggtg ccgatggccg agctctgcta cagtcctgta cagttcaggt
     3841 gacacaggcc ccaggaggct gggaagtcct ggctgttgtg gtccctgtgc ccccctttac
     3901 ctgcctgctc cgggacctgg tgcctgccac caactacagc ctcagggtgc gctgtgccaa
     3961 tgccttgggg ccctctccct atgctgactg ggtgcccttt cagaccaagg gtctagcccc
     4021 agccagcgct ccccaaaacc tccatgccat ccgcacagat tcaggcctca tcttggagtg
     4081 ggaagaagtg atccccgagg cccctttgga aggccccctg ggaccctaca aactgtcctg
     4141 ggttcaagac aatggaaccc aggatgagct gacagtggag gggaccaggg ccaatttgac
     4201 aggctgggat ccccaaaagg acctgatcgt acgtgtgtgc gtctccaatg cagttggctg
     4261 tggaccctgg agtcagccac tggtggtctc ttctcatgac cgtgcaggcc agcagggccc
     4321 tcctcacagc cgcacatcct gggtacctgt ggtccttggt gtgctaacgg ccctggtgac
     4381 ggctgctgcc ctggccctca tcctgcttcg aaagagacgg aaagagacgc ggtttgggca
     4441 agcctttgac agtgtcatgg cccggggaga gccagccgtt cacttccggg cagcccggtc
     4501 cttcaatcga gaaaggcccg agcgcatcga ggccacattg gacagcttgg gcatcagcga
     4561 tgaactaaag gaaaaactgg aggatgtgct catcccagag cagcagttca ccctgggccg
     4621 gatgttgggc aaaggagagt ttggttcagt gcgggaggcc cagctgaagc aagaggatgg
     4681 ctcctttgtg aaagtggctg tgaagatgct gaaagctgac atcattgcct caagcgacat
     4741 tgaagagttc ctcagggaag cagcttgcat gaaggagttt gaccatccac acgtggccaa
     4801 acttgttggg gtaagcctcc ggagcagggc taaaggccgt ctcccaatcc ccatggtcat
     4861 cttgcccttc atgaagcatg gggacctgca tgccttcctg ctcgcctccc ggattgggga
     4921 gaaccccttt aacctacccc tccagaccct gatccggttc atggtggaca ttgcctgcgg
     4981 catggagtac ctgagctctc ggaacttcat ccaccgagac ctggctgctc ggaattgcat
     5041 gctggcagag gacatgacag tgtgtgtggc tgacttcgga ctctcccgga agatctacag
     5101 tggggactac tatcgtcaag gctgtgcctc caaactgcct gtcaagtggc tggccctgga
     5161 gagcctggcc gacaacctgt atactgtgca gagtgacgtg tgggcgttcg gggtgaccat
     5221 gtgggagatc atgacacgtg ggcagacgcc atatgctggc atcgaaaacg ctgagattta
     5281 caactacctc attggcggga accgcctgaa acagcctccg gagtgtatgg aggacgtgta
     5341 tgatctcatg taccagtgct ggagtgctga ccccaagcag cgcccgagct ttacttgtct
     5401 gcgaatggaa ctggagaaca tcttgggcca gctgtctgtg ctatctgcca gccaggaccc
     5461 cttatacatc aacatcgaga gagctgagga gcccactgcg ggaggcagcc tggagctacc
     5521 tggcagggat cagccctaca gtggggctgg ggatggcagt ggcatggggg cagtgggtgg
     5581 cactcccagt gactgtcggt acatactcac ccccggaggg ctggctgagc agccagggca
     5641 ggcagagcac cagccagaga gtcccctcaa tgagacacag aggcttttgc tgctgcagca
     5701 agggctactg ccacacagta gctgtgaccc agctttcttg tacaaagtgg ttgatatcaa
     5761 ggccgccgac atcaccagcc tgtacaagaa agtggggtgg agccatcccc agttcgagaa
     5821 gggcggagga tctggcggcg gaagcggcgg aggaagctgg tcccaccctc agtttgagaa
     5881 aggcaccgag ctgggcagca ccatggccag ctacccctac gacgtgcccg actacgccaa
     5941 ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt tccactctgg
     6001 cgagcagctg ggcgagacac tgggcatgag cagagccgcc atcaacaagc acatccagac
     6061 cctgcgggac tggggcgtgg acgtgttcac agtgcccggc aagggctaca gcctgcccga
     6121 gcccatccag ctgctgaacg ccaagcagat cctgggccag ctggatggcg gatctgtggc
     6181 cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc gaccggatcg gcgagctgaa
     6241 gtccggcgac gcctgtatcg ccgagtacca gcaggccggc agaggcggca ggggcagaaa
     6301 gtggttcagc cccttcggcg ccaacctgta cctgagcatg ttctggagac tggaacaggg
     6361 cccagccgcc gctattggcc tgagcctggt catcggcatc gtgatggccg aggtgctgcg
     6421 gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac gacctgtatc tgcaggaccg
     6481 gaagctggcc ggcatcctgg tggaactgac cggcaaaaca ggcgacgccg cccagatcgt
     6541 gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa gagagcgtgg tcaaccaggg
     6601 ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg aacaccctgg ccgccatgct
     6661 gatcagagag ctgagagccg ccctggaact gttcgagcag gaaggcctgg ccccctacct
     6721 gagcagatgg gagaagctgg acaacttcat caaccggccc gtgaaactga tcatcggcga
     6781 caaagagatc ttcggcatca gccggggcat cgacaagcag ggggccctgc tcctggaaca
     6841 ggacggcatc atcaagccct ggatgggcgg cgagatcagc ctgcggagcg ccgagaagtg
     6901 atgactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6961 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7021 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7081 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7141 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7201 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7261 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7321 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7381 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7441 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     7501 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     7561 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     7621 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     7681 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     7741 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     7801 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     7861 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     7921 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     7981 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     8041 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     8101 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     8161 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     8221 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     8281 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     8341 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     8401 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     8461 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     8521 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     8581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8641 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8701 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8761 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
//
