LOCUS       sequence-372416-        8805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human NTRK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_NTRK3
FEATURES             Location/Qualifiers
     source          1..8805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..45)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(199..787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(279..298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(958..1749,1750..1818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1581..1600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1819..1923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1998..2017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2189..2568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2569..2772
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2723..2743
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2774..2792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2795..2813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2823..2847
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2990..3014
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5496..5520)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5567..5590
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5627..5650
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5681..5707
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5681..5707
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5708..6055,6056..6058,6059..6667)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6711..6728)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6717..6941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7074..7093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7225..7272
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7280..8302
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7280..8302
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8432..8553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8469..8488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8523..8542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8602..8618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8602..8618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8615..8637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8626..8642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8650..8656,8657..8674,8675..8680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8695..8716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
       61 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1921 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     1981 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     2041 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     2101 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     2161 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     2221 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2281 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2341 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2401 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2461 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2521 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2581 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2641 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2701 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2761 ataagcagag ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga
     2821 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     2881 catagaagac accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt
     2941 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     3001 caaaaaagca ggctccacca tggatgtctc tctttgccca gccaagtgta gtttctggcg
     3061 gattttcttg ctgggaagcg tctggctgga ctatgtgggc tccgtgctgg cttgccctgc
     3121 aaattgtgtc tgcagcaaga ctgagatcaa ttgccggcgg ccggacgatg ggaacctctt
     3181 ccccctcctg gaagggcagg attcagggaa cagcaatggg aacgccagta tcaacatcac
     3241 ggacatctca aggaatatca cttccataca catagagaac tggcgcagtc ttcacacgct
     3301 caacgccgtg gacatggagc tctacaccgg acttcaaaag ctgaccatca agaactcagg
     3361 acttcggagc attcagccca gagcctttgc caagaacccc catttgcgtt atataaacct
     3421 gtcaagtaac cggctcacca cactctcgtg gcagctcttc cagacgctga gtcttcggga
     3481 attgcagttg gagcagaact ttttcaactg cagctgtgac atccgctgga tgcagctctg
     3541 gcaggagcag ggggaggcca agctcaacag ccagaacctc tactgcatca acgctgatgg
     3601 ctcccagctt cctctcttcc gcatgaacat cagtcagtgt gaccttcctg agatcagcgt
     3661 gagccacgtc aacctgaccg tacgagaggg tgacaatgct gttatcactt gcaatggctc
     3721 tggatcaccc cttcctgatg tggactggat agtcactggg ctgcagtcca tcaacactca
     3781 ccagaccaat ctgaactgga ccaatgttca tgccatcaac ttgacgctgg tgaatgtgac
     3841 gagtgaggac aatggcttca ccctgacgtg cattgcagag aacgtggtgg gcatgagcaa
     3901 tgccagtgtt gccctcactg tctactatcc cccacgtgtg gtgagcctgg aggagcctga
     3961 gctgcgcctg gagcactgca tcgagtttgt ggtgcgtggc aaccccccac caacgctgca
     4021 ctggctgcac aatgggcagc ctctgcggga gtccaagatc atccatgtgg aatactacca
     4081 agagggagag atttccgagg gctgcctgct cttcaacaag cccacccact acaacaatgg
     4141 caactatacc ctcattgcca aaaacccact gggcacagcc aaccagacca tcaatggcca
     4201 cttcctcaag gagccctttc cagagagcac ggataacttt atcttgtttg acgaagtgag
     4261 tcccacacct cctatcactg tgacccacaa accagaagaa gacacttttg gggtatccat
     4321 agcagttgga cttgctgctt ttgcctgtgt cctgttggtg gttctcttcg tcatgatcaa
     4381 caaatatggt cgacggtcca aatttggaat gaagggtccc gtggctgtca tcagtggtga
     4441 ggaggactca gccagcccac tgcaccacat caaccacggc atcaccacgc cctcgtcact
     4501 ggatgccggg cccgacactg tggtcattgg catgactcgc atccctgtca ttgagaaccc
     4561 ccagtacttc cgtcagggac acaactgcca caagccggac acgtatgtgc agcacattaa
     4621 gaggagagac atcgtgctga agcgagaact gggtgaggga gcctttggaa aggtcttcct
     4681 ggccgagtgc tacaacctca gcccgaccaa ggacaagatg cttgtggctg tgaaggccct
     4741 gaaggatccc accctggctg cccggaagga tttccagagg gaggccgagc tgctcaccaa
     4801 cctgcagcat gagcacattg tcaagttcta tggagtgtgc ggcgatgggg accccctcat
     4861 catggtcttt gaatacatga agcatggaga cctgaataag ttcctcaggg cccatgggcc
     4921 agatgcaatg atccttgtgg atggacagcc acgccaggcc aagggtgagc tggggctctc
     4981 ccaaatgctc cacattgcca gtcagatcgc ctcgggtatg gtgtacctgg cctcccagca
     5041 ctttgtgcac cgagacctgg ccaccaggaa ctgcctggtt ggagcgaatc tgctagtgaa
     5101 gattggggac ttcggcatgt ccagagatgt ctacagcacg gattattaca gggtgggagg
     5161 acacaccatg ctccccattc gctggatgcc tcctgaaagc atcatgtacc ggaagttcac
     5221 tacagagagt gatgtatgga gcttcggggt gatcctctgg gagatcttca cctatggaaa
     5281 gcagccatgg ttccaactct caaacacgga ggtcattgag tgcattaccc aaggtcgtgt
     5341 tttggagcgg ccccgagtct gccccaaaga ggtgtacgat gtcatgctgg ggtgctggca
     5401 gagggaacca cagcagcggt tgaacatcaa ggagatctac aaaatcctcc atgctttggg
     5461 gaaggccacc ccaatctacc tggacattct tggcgaccca gctttcttgt acaaagtggt
     5521 tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga gccatcccca
     5581 gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt cccaccctca
     5641 gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg acgtgcccga
     5701 ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt
     5761 ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca tcaacaagca
     5821 catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca agggctacag
     5881 cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc tggatggcgg
     5941 atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg accggatcgg
     6001 cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca gaggcggcag
     6061 gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggagact
     6121 ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg tgatggccga
     6181 ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct
     6241 gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc
     6301 ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag agagcgtggt
     6361 caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga acaccctggc
     6421 cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg aaggcctggc
     6481 cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg tgaaactgat
     6541 catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg gggccctgct
     6601 cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc
     6661 cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     6721 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6781 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6841 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6901 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6961 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7201 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     7261 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     7321 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     7381 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     7441 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     7501 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     7561 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     7621 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     7681 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     7741 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     7801 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     7861 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     7921 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     7981 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     8041 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     8101 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     8161 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     8221 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     8281 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     8341 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8401 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8461 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8521 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8581 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcgg
//
