LOCUS       sequence-372498-        9014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 
            around GFP for subcloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, 
            Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella 
            BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, 
            Ianari A
  TITLE     Systematic profiling of conditional degron tag technologies for 
            target validation studies.
  JOURNAL   Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4.
  PUBMED    36127368
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-20"; volume: "13(1):5495. doi"; pages: " 
            10.1038/s41467-022-33246-4"
COMMENT     Sequence Label: PGK-SMASH-CTERM-GFP
FEATURES             Location/Qualifiers
     source          1..9014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        241..259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             514..694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1359..1592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1777..1821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2012..2512
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2431..2449
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3194..3250
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3257..3259,3260..3970)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             3977..4018
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4957..5074
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5153..5741
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5206..5226)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5624..5635)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5813..6046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6124..6245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6161..6180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6215..6234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6285..6420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6440..6459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6441..6459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6469..6486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6469..6485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6478..6500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6627..7082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6714..6733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6924..6945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7108..7212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7213..7281,7282..8073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7431..7450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8244..8832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8733..8752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8986..9003
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1981 accccgaggg gaccctattc cagcacatat gttgcgcctt ttccaaggca gccctgggtt
     2041 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     2101 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     2161 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     2221 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     2281 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     2341 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     2401 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     2461 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcagactga
     2521 gtcggccggt cgaatcaagc ttatcgatac cgtcgactcc ggaatagcca ccatagccac
     2581 cgccaccatg accgagtaca agccaactgt ccgcttggca acccgcgacg atgtgcccag
     2641 ggccgtccgc acgctggctg ctgccttcgc cgactaccca gccacccgcc acaccgtgga
     2701 cccggaccgc cacatcgagc gcgtcaccga gctgcaggag ctcttcctca cccgagttgg
     2761 ccttgacatt ggcaaggtct gggtggcgga cgacggtgct gccgtggccg tctggaccac
     2821 ccccgagtcg gtcgaggctg gcgccgtgtt cgccgagatc gggccgcgca tggccgagct
     2881 ttcgggctcc cggttggctg cccaacagca gatggagggg ctgttggcac cccaccgccc
     2941 caaggagccg gcctggttcc tggccacagt tggtgtctct cccgaccacc agggcaaggg
     3001 cctgggcagc gccgtggtgc tgcctggggt cgaggctgcc gagcgcgctg gcgtccctgc
     3061 cttcctggag acctctgccc ctcgcaactt gcccttctac gagcgcctgg gcttcaccgt
     3121 caccgcggac gtcgaggtgc ccgaggggcc gcgcacctgg tgcatgaccc gcaagcctgg
     3181 ggcaggctcc ggagccacca acttttccct gctgaagcag gctggcgacg tcgaggagaa
     3241 ccctggccct ggatccgtca gtaaagggga agaattgttt acgggtgttg ttccgatact
     3301 tgttgaattg gatggggatg tgaatggtca taaattttcc gttagtggtg aaggggaagg
     3361 tgacgctact tatggtaaat tgacattgaa atttatttgt actacaggaa aacttccagt
     3421 cccatggccg actttggtaa cgactcttac atatggagtc caatgtttta gtaggtatcc
     3481 agatcacatg aaacaacatg atttctttaa atcagcaatg cctgagggat atgttcaaga
     3541 acgaacaata ttctttaaag atgatgggaa ctataagaca agggcagaag tcaaatttga
     3601 aggtgataca ctcgttaatc ggattgaact gaaagggata gattttaagg aagatgggaa
     3661 tattcttggg cacaaacttg aatataatta taattcccat aatgtataca taatggctga
     3721 caagcagaag aatggaatta aagtcaattt taaaataagg cataatatag aagatgggag
     3781 tgtccaattg gctgatcatt accaacaaaa tacacctatt ggggatggtc ctgtcctctt
     3841 gcctgataat cattatcttt ctacacaatc tgctctctca aaggacccta atgaaaagcg
     3901 cgaccatatg gtgttgttgg aatttgtcac tgctgcaggc attaccctgg gaatggatga
     3961 actctataaa gaattcggta agcctatccc taaccctctc ctcggtctcg attctacgga
     4021 tgagatggaa gagtgctctc agcacttagc tgaagctgct gcaaaggaag ctgcagctaa
     4081 ggaggctgca gctaaggctg cgcccatcac ggcgtacgcc cagcagacga gaggcctcct
     4141 agggtgtata atcaccagcc tgactggccg ggacaaaaac caagtggagg gtgaggtcca
     4201 gatcgtgtca actgctaccc aaaccttcct ggcaacgtgc atcaatgggg tatgctgggc
     4261 agtctaccac ggggccggaa cgaggaccat cgcatcaccc aagggtcctg tcatccagat
     4321 gtataccaat gtggaccaag accttgtggg ctggcccgct cctcaaggtt cccgctcatt
     4381 gacaccctgt acctgcggct cctcggacct ttacctggtc acgaggcacg ccgatgtcat
     4441 tcccgtgcgc cggcgaggtg atagcagggg tagcctgctt tcgccccggc ccatttccta
     4501 cttgaaaggc tcctctgggg gtccgctgtt gtgccccgcg ggacacgccg tgggcctatt
     4561 cagggccgcg gtgtgcaccc gtggagtggc taaagcggtg gactttatcc ctgtggagaa
     4621 cctagagaca accatgagat ccccggtgtt cacggacaac tcctctccac cagcagtcac
     4681 cctgacgcac ccaatcacca aaatcgatac caaatacatc atgacatgca tgtcggccga
     4741 cctggaggtc gtcacgagca cctgggtgct cgttggcggc gtcctggctg ctctggccgc
     4801 gtattgcctg tcaacaggct gcgtggtcat agtgggcagg atcgtcttgt ccgggaagcc
     4861 ggcaattata cctgacaggg aggttctcta ctaggtacaa gtaaactaag cttggcgtaa
     4921 ctagaacttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4981 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5041 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     5101 tccactttgg gctcgagctt ggttcattct caagcctacg cgttaagtcg acaatcaacc
     5161 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5221 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5281 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5341 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5401 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5461 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5521 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5581 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5641 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5701 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     5761 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5821 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     6061 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6121 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6241 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6301 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6361 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6421 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6481 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6541 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6601 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6841 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6901 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6961 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7021 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7081 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7441 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7741 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9001 gaggaagcgg aaga
//
