LOCUS       sequence-372503-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 
            around GFP for subcloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, 
            Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella 
            BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, 
            Ianari A
  TITLE     Systematic profiling of conditional degron tag technologies for 
            target validation studies.
  JOURNAL   Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4.
  PUBMED    36127368
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-20"; volume: "13(1):5495. doi"; pages: " 
            10.1038/s41467-022-33246-4"
COMMENT     Sequence Label: PGK-SMASH-NTERM-GFP
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        241..259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        287..513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             514..694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    741..866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1359..1592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1777..1821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2012..2512
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2431..2449
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3194..3250
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4097..4138
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             join(4196..4198,4199..4909)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    4993..5110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5189..5777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5242..5262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5660..5671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5849..6082
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6160..6281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6197..6216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6251..6270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6321..6456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6476..6495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6477..6495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6505..6522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6505..6521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6514..6536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6663..7118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6750..6769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6960..6981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7144..7248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7249..7317,7318..8109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7467..7486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8280..8868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8769..8788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9022..9039
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1981 accccgaggg gaccctattc cagcacatat gttgcgcctt ttccaaggca gccctgggtt
     2041 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     2101 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     2161 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     2221 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     2281 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     2341 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     2401 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     2461 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcagactga
     2521 gtcggccggt cgaatcaagc ttatcgatac cgtcgactcc ggaatagcca ccatagccac
     2581 cgccaccatg accgagtaca agccaactgt ccgcttggca acccgcgacg atgtgcccag
     2641 ggccgtccgc acgctggctg ctgccttcgc cgactaccca gccacccgcc acaccgtgga
     2701 cccggaccgc cacatcgagc gcgtcaccga gctgcaggag ctcttcctca cccgagttgg
     2761 ccttgacatt ggcaaggtct gggtggcgga cgacggtgct gccgtggccg tctggaccac
     2821 ccccgagtcg gtcgaggctg gcgccgtgtt cgccgagatc gggccgcgca tggccgagct
     2881 ttcgggctcc cggttggctg cccaacagca gatggagggg ctgttggcac cccaccgccc
     2941 caaggagccg gcctggttcc tggccacagt tggtgtctct cccgaccacc agggcaaggg
     3001 cctgggcagc gccgtggtgc tgcctggggt cgaggctgcc gagcgcgctg gcgtccctgc
     3061 cttcctggag acctctgccc ctcgcaactt gcccttctac gagcgcctgg gcttcaccgt
     3121 caccgcggac gtcgaggtgc ccgaggggcc gcgcacctgg tgcatgaccc gcaagcctgg
     3181 ggcaggctcc ggagccacca acttttccct gctgaagcag gctggcgacg tcgaggagaa
     3241 ccctggccct gcgcccatca cggcgtacgc ccagcagacg agaggcctcc tagggtgtat
     3301 aatcaccagc ctgactggcc gggacaaaaa ccaagtggag ggtgaggtcc agatcgtgtc
     3361 aactgctacc caaaccttcc tggcaacgtg catcaatggg gtatgctggg cagtctacca
     3421 cggggccgga acgaggacca tcgcatcacc caagggtcct gtcatccaga tgtataccaa
     3481 tgtggaccaa gaccttgtgg gctggcccgc tcctcaaggt tcccgctcat tgacaccctg
     3541 tacctgcggc tcctcggacc tttacctggt cacgaggcac gccgatgtca ttcccgtgcg
     3601 ccggcgaggt gatagcaggg gtagcctgct ttcgccccgg cccatttcct acttgaaagg
     3661 ctcctcgggg ggtccgctgt tgtgccccgc gggacacgcc gtgggcctat tcagggccgc
     3721 ggtgtgcacc cgtggagtgg ctaaagcggt ggactttatc cctgtggaga acctagagac
     3781 aaccatgaga tccccggtgt tcacggacaa ctcctctcca ccagcagtca ccctgacgca
     3841 cccaatcacc aaaatcgata ccaaatacat catgacatgc atgtcggccg acctggaggt
     3901 cgtcacgagc acctgggtgc tcgttggcgg cgtcctggct gctctggccg cgtattgcct
     3961 gtcaacaggc tgcgtggtca tagtgggcag gatcgtcttg tccgggaagc cggcaggcag
     4021 tagcggaagc agtattatac ctgacaggga ggttctctac caggagttcg aagatgtcgt
     4081 gccatgctca atgggcggta agcctatccc taaccctctc ctcggtctcg attctacggc
     4141 tgaagctgct gcaaaggaag ctgcagctaa ggaggctgca gctaaggctg gatccgtcag
     4201 taaaggggaa gaattgttta cgggtgttgt tccgatactt gttgaattgg atggggatgt
     4261 gaatggtcat aaattttccg ttagtggtga aggggaaggt gacgctactt atggtaaatt
     4321 gacattgaaa tttatttgta ctacaggaaa acttccagtc ccatggccga ctttggtaac
     4381 gactcttaca tatggagtcc aatgttttag taggtatcca gatcacatga aacaacatga
     4441 tttctttaaa tcagcaatgc ctgagggata tgttcaagaa cgaacaatat tctttaaaga
     4501 tgatgggaac tataagacaa gggcagaagt caaatttgaa ggtgatacac tcgttaatcg
     4561 gattgaactg aaagggatag attttaagga agatgggaat attcttgggc acaaacttga
     4621 atataattat aattcccata atgtatacat aatggctgac aagcagaaga atggaattaa
     4681 agtcaatttt aaaataaggc ataatataga agatgggagt gtccaattgg ctgatcatta
     4741 ccaacaaaat acacctattg gggatggtcc tgtcctcttg cctgataatc attatctttc
     4801 tacacaatct gctctctcaa aggaccctaa tgaaaagcgc gaccatatgg tgttgttgga
     4861 atttgtcact gctgcaggca ttaccctggg aatggatgaa ctctataaat gagaattcta
     4921 gggctccgga gtacaagtaa actaagcttg gcgtaactag atcttgagac aaatggcagt
     4981 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     5041 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     5101 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttgggctc gagcttggtt
     5161 cattctcaag cctacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     5221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5581 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5761 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     5821 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5881 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6061 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     6121 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6181 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6241 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6481 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6541 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6601 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6661 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6721 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6781 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6841 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6901 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6961 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7021 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7081 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8401 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8941 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9001 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
