LOCUS       sequence-372555-       13695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-BirA 
            3xFLAG-NUP37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK BirA* 3xFLAG-NUP37 zeo
FEATURES             Location/Qualifiers
     source          1..13695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2991..3341,3342..3344,3345..3953)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3963..4028
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5045..5069)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5119..6330
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6167..6186
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6389..7006,7007..7012,7013..7048,7049..7051,7052..7087,
                     7088..7090,7091..7126,7127..7135)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7173..7747
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7340..7357)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        7769..7816
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7835..8209
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8383..8971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8436..8456)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8854..8865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8974..8990)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8975..8991)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9500..9680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9706..9723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9712..9936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9982..10410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10069..10088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10279..10300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10419..10439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10424..10753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10604..10739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10666..10685
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10801..10848
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10867..11241
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11371..11492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11408..11427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11462..11481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11541..11557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11541..11557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11554..11576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11565..11581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11589..11595,11596..11613,11614..11619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11634..11655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11772..11789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11943..12531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12023..12042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12702..13493,13494..13562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13325..13344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13563..13667)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga atgaaggaca
     3001 acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc
     3061 agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc cagacactga
     3121 gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta
     3181 tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc gtggccgtgc
     3241 tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag ctgaagtccg
     3301 gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga cggaagtggt
     3361 tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag cagggacctg
     3421 ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc
     3481 tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag gaccgcaagc
     3541 tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag atcgtgatcg
     3601 gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac cagggctgga
     3661 tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc atgctgatca
     3721 gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct tacctgagca
     3781 gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc ggcgacaagg
     3841 aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg gagcaggacg
     3901 gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag aagactagta
     3961 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     4021 atgacaagat gaagcaagat gcctcaagaa atgctgccta cactgtggat tgtgaagatt
     4081 atgtgcatgt ggtagaattt aatccctttg agaatgggga ttcaggaaac ctaattgcat
     4141 atggtggcaa taattatgtg gtcattggca cgtgtacgtt tcaggaagaa gaagcagacg
     4201 ttgaaggcat tcagtataaa acacttcgaa catttcacca tggagtcagg gttgatggca
     4261 tagcttggag cccagagact agacttgatt cattgcctcc agtaatcaaa ttttgtactt
     4321 cagctgctga tatgaaaatt agattattta cttcagatct tcaggataaa aatgaatata
     4381 aggttttaga gggccatacc gatttcatta atggtttggt gtttgatccc aaagaaggcc
     4441 aagaaattgc aagtgtgagt gacgatcaca cctgcaggat ttggaacttg gaaggagtgc
     4501 aaacagctca ttttgttctt cattctcctg gcatgagtgt gtgctggcat cctgaggaga
     4561 cttttaagct aatggttgca gagaagaatg gaacaatccg gttttatgat cttttggccc
     4621 aacaggctat tttatctctt gaatcagaac aagtgccatt aatgtcagca cactggtgct
     4681 taaaaaacac cttcaaagtt ggagccgttg caggaaatga ttggttaatt tgggatatta
     4741 ctcggtccag ttatcctcaa aataagagac ctgttcacat ggatcgagcc tgcttattca
     4801 ggtggtccac aattagtgaa aatctgtttg caaccactgg ttatcctggc aaaatggcaa
     4861 gccagtttca aattcatcat ttaggacacc ctcagcccat cctcatgggt tctgtagccg
     4921 ttggatctgg actgtcctgg catcgaactc tccctctgtg tgtaattgga ggagaccaca
     4981 agctgttgtt ttgggtgact gaagtataac aattggaaga ctaatgcgtt ttcgagatat
     5041 ctagacccag ctttcttgta caaagtggtt gataacatct cgagccataa agatggttaa
     5101 ttaacccacc caagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     5161 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     5221 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     5281 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     5341 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     5401 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     5461 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     5521 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     5581 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     5641 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     5701 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     5761 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     5821 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     5881 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     5941 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     6001 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     6061 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     6121 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     6181 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     6241 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     6301 ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg gatctccacc
     6361 ccgtaccggt cctgcagtcg aattcaccat gtctagactg gacaagagca aagtcataaa
     6421 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     6481 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     6541 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     6601 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     6661 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     6721 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     6781 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     6841 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     6901 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     6961 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     7021 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     7081 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     7141 taaggatccg cggccgcact agaggaattc cgcccctctc cctccccccc ccctaacgtt
     7201 actggccgaa gccgcttgga ataaggccgg tgtgtgtttg tctatatgtt attttccacc
     7261 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     7321 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     7381 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     7441 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     7501 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7561 gtcaaatggc tctcctcaag cgtagtcaac aaggggctga aggatgccca gaaggtaccc
     7621 cattgtatgg gaatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7681 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7741 atgataagct taccggtacg cgtgatgtgt tgacaattaa tcatcggcat agtatatcgg
     7801 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     7861 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     7921 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     7981 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     8041 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     8101 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     8161 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acatctgtac
     8221 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     8281 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8341 ttgttgttta gtccctccca attcgatatc aagcttatcg ataatcaacc tctggattac
     8401 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8461 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8521 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8581 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8641 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8701 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8761 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8821 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8881 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8941 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     9001 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     9061 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     9121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9181 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     9241 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     9301 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     9361 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9421 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     9481 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9661 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9721 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     9781 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     9841 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     9901 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9961 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    10021 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    10081 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    10141 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    10201 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    10261 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    10321 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    10381 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
    10441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10501 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10561 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10681 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10741 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
    10801 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
    10861 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
    10921 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
    10981 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
    11041 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
    11101 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
    11161 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
    11221 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
    11281 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    11341 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    11401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    11521 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11581 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11641 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11701 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    11761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    11821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    11941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    12241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    12301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    12361 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    12421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12721 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    12781 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    12841 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    12901 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    12961 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13021 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13081 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13141 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13201 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13261 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    13321 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    13381 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    13441 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    13501 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    13561 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13621 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13681 aaagtgccac ctgac
//
