LOCUS       sequence-372575-        9369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human EPHA8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_EPHA8
FEATURES             Location/Qualifiers
     source          1..9369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        520..723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    725..743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    746..764
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     774..798
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    941..965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4011..4035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4082..4105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4142..4165
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4196..4222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4196..4222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4223..4570,4571..4573,4574..5182)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5226..5243)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5232..5456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5589..5608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5740..5787
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5795..6817
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5795..6817
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6947..7068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6984..7003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7038..7057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7117..7133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7117..7133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7130..7152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7141..7157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7165..7171,7172..7189,7190..7195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7210..7231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7348..7365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7519..8107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7599..7618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8278..9069,9070..9138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8901..8920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9139..9243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9318..9337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
       61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      721 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
      781 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      841 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
      901 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
      961 agaagggccg tcaaggccca ccatggcccc cgcccggggc cgcctgcccc ctgcgctctg
     1021 ggtcgtcacg gccgcggcgg cggcggccac ctgcgtgtcc gcggcgcgcg gcgaagtgaa
     1081 tttgctggac acgtcgacca tccacgggga ctggggctgg ctcacgtatc cggctcatgg
     1141 gtgggactcc atcaacgagg tggacgagtc cttccagccc atccacacgt accaggtttg
     1201 caacgtcatg agccccaacc agaacaactg gctgcgcacg agctgggtcc cccgagacgg
     1261 cgcccggcgc gtctatgctg agatcaagtt taccctgcgc gactgcaaca gcatgcctgg
     1321 tgtgctgggc acctgcaagg agaccttcaa cctctactac ctggagtcgg accgcgacct
     1381 gggggccagc acacaagaaa gccagttcct caaaatcgac accattgcgg ccgacgagag
     1441 cttcacaggt gccgaccttg gtgtgcggcg tctcaagctc aacacggagg tgcgcagtgt
     1501 gggtcccctc agcaagcgcg gcttctacct ggccttccag gacataggtg cctgcctggc
     1561 catcctctct ctccgcatct actataagaa gtgccctgcc atggtgcgca atctggctgc
     1621 cttctcggag gcagtgacgg gggccgactc gtcctcactg gtggaggtga ggggccagtg
     1681 cgtgcggcac tcagaggagc gggacacacc caagatgtac tgcagcgcgg agggcgagtg
     1741 gctcgtgccc atcggcaaat gcgtgtgcag tgccggctac gaggagcggc gggatgcctg
     1801 tgtggcctgt gagctgggct tctacaagtc agcccctggg gaccagctgt gtgcccgctg
     1861 ccctccccac agccactccg cagctccagc cgcccaagcc tgccactgtg acctcagcta
     1921 ctaccgtgca gccctggacc cgccgtcctc agcctgcacc cggccaccct cggcaccagt
     1981 gaacctgatc tccagtgtga atgggacatc agtgactctg gagtgggccc ctcccctgga
     2041 cccaggtggc cgcagtgaca tcacctacaa tgccgtgtgc cgccgctgcc cctgggcact
     2101 gagccgctgc gaggcatgtg ggagcggcac ccgctttgtg ccccagcaga caagcctggt
     2161 gcaggccagc ctgctggtgg ccaacctgct ggcccacatg aactactcct tctggatcga
     2221 ggccgtcaat ggcgtgtccg acctgagccc cgagccccgc cgggccgctg tggtcaacat
     2281 caccacgaac caggcagccc cgtcccaggt ggtggtgatc cgtcaagagc gggcggggca
     2341 gaccagcgtc tcgctgctgt ggcaggagcc cgagcagccg aacggcatca tcctggagta
     2401 tgagatcaag tactacgaga aggacaagga gatgcagagc tactccaccc tcaaggccgt
     2461 caccaccaga gccaccgtct ccggcctcaa gccgggcacc cgctacgtgt tccaggtccg
     2521 agcccgcacc tcagcaggct gtggccgctt cagccaggcc atggaggtgg agaccgggaa
     2581 accccggccc cgctatgaca ccaggaccat tgtctggatc tgcctgacgc tcatcacggg
     2641 cctggtggtg cttctgctcc tgctcatctg caagaagagg cactgtggct acagcaaggc
     2701 cttccaggac tcggacgagg agaagatgca ctatcagaat ggacaggcac ccccacctgt
     2761 cttcctgcct ctgcatcacc ccccgggaaa gctcccagag ccccagttct atgcggaacc
     2821 ccacacctac gaggagccag gccgggcggg ccgcagtttc actcgggaga tcgaggcctc
     2881 taggatccac atcgagaaaa tcatcggctc tggagactcc ggggaagtct gctacgggag
     2941 gctgcgggtg ccagggcagc gggatgtgcc cgtggccatc aaggccctca aagccggcta
     3001 cacggagaga cagaggcggg acttcctgag cgaggcgtcc atcatggggc aattcgacca
     3061 tcccaacatc atccgcctcg agggtgtcgt cacccgtggc cgcctggcaa tgattgtgac
     3121 tgagtacatg gagaacggct ctctggacac cttcctgagg acccacgacg ggcagttcac
     3181 catcatgcag ctggtgggca tgctgagagg agtgggtgcc ggcatgcgct acctctcaga
     3241 cctgggctat gtccaccgag acctggccgc ccgcaacgtc ctggttgaca gcaacctggt
     3301 ctgcaaggtg tctgacttcg ggctctcacg ggtgctggag gacgacccgg atgctgccta
     3361 caccaccacg ggcgggaaga tccccatccg ctggacggcc ccagaggcca tcgccttccg
     3421 caccttctcc tcggccagcg acgtgtggag cttcggcgtg gtcatgtggg aggtgctggc
     3481 ctatggggag cggccctact ggaacatgac caaccgggat gtcatcagct ctgtggagga
     3541 ggggtaccgc ctgcccgcac ccatgggctg cccccacgcc ctgcaccagc tcatgctcga
     3601 ctgttggcac aaggaccggg cgcagcggcc tcgcttctcc cagattgtca gtgtcctcga
     3661 tgcgctcatc cgcagccctg agagtctcag ggccaccgcc acagtcagca ggtgcccacc
     3721 ccctgccttc gtccggagct gctttgacct ccgagggggc agcggtggcg gtgggggcct
     3781 caccgtgggg gactggctgg actccatccg catgggccgg taccgagacc acttcgctgc
     3841 gggcggatac tcctctctgg gcatggtgct acgcatgaac gcccaggacg tgcgcgccct
     3901 gggcatcacc ctcatgggcc accagaagaa gatcctgggc agcattcaga ccatgcgggc
     3961 ccagctgacc agcacccagg ggccccgccg gcacctctca ggcctcatgg gcccagcttt
     4021 cttgtacaaa gtggttgata tcaaggccgc cgacatcacc agcctgtaca agaaagtggg
     4081 gtggagccat ccccagttcg agaagggcgg aggatctggc ggcggaagcg gcggaggaag
     4141 ctggtcccac cctcagtttg agaaaggcac cgagctgggc agcaccatgg ccagctaccc
     4201 ctacgacgtg cccgactacg ccaaggacaa caccgtgccc ctgaagctga tcgccctgct
     4261 ggccaacggc gagttccact ctggcgagca gctgggcgag acactgggca tgagcagagc
     4321 cgccatcaac aagcacatcc agaccctgcg ggactggggc gtggacgtgt tcacagtgcc
     4381 cggcaagggc tacagcctgc ccgagcccat ccagctgctg aacgccaagc agatcctggg
     4441 ccagctggat ggcggatctg tggccgtgct gcccgtgatc gacagcacca accagtatct
     4501 gctcgaccgg atcggcgagc tgaagtccgg cgacgcctgt atcgccgagt accagcaggc
     4561 cggcagaggc ggcaggggca gaaagtggtt cagccccttc ggcgccaacc tgtacctgag
     4621 catgttctgg agactggaac agggcccagc cgccgctatt ggcctgagcc tggtcatcgg
     4681 catcgtgatg gccgaggtgc tgcggaagct gggcgccgac aaagtgcgcg tgaagtggcc
     4741 caacgacctg tatctgcagg accggaagct ggccggcatc ctggtggaac tgaccggcaa
     4801 aacaggcgac gccgcccaga tcgtgatcgg cgctggcatc aacatggcca tgcggcgggt
     4861 ggaagagagc gtggtcaacc agggctggat caccctgcag gaagccggca tcaacctgga
     4921 ccggaacacc ctggccgcca tgctgatcag agagctgaga gccgccctgg aactgttcga
     4981 gcaggaaggc ctggccccct acctgagcag atgggagaag ctggacaact tcatcaaccg
     5041 gcccgtgaaa ctgatcatcg gcgacaaaga gatcttcggc atcagccggg gcatcgacaa
     5101 gcagggggcc ctgctcctgg aacaggacgg catcatcaag ccctggatgg gcggcgagat
     5161 cagcctgcgg agcgccgaga agtgatgact cgagtctaga gggcccgttt aaacccgctg
     5221 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5281 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5341 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5401 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5461 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5701 ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc
     5761 ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc
     5821 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5881 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5941 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     6001 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     6061 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     6121 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     6181 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     6241 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     6301 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     6361 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     6421 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     6481 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     6541 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     6601 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     6661 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6721 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6781 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc
     6841 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6901 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7081 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8401 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8581 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8761 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9241 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     9301 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     9361 cctgcttgt
//
