LOCUS       sequence-372725-       10797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human LMTK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_LMTK3
FEATURES             Location/Qualifiers
     source          1..10797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..28)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        200..579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        580..783
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     734..754
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    785..803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    806..824
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     834..858
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1001..1025
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5499..5523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5570..5593
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5630..5653
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5684..5710
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5684..5710
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5711..6058,6059..6061,6062..6670)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     polyA_signal    6720..6944
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7077..7096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7228..7275
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7283..8305
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7283..8305
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8435..8556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8472..8491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8526..8545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8605..8621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8605..8621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8618..8640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8629..8645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8653..8659,8660..8677,8678..8683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8698..8719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8836..8853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9007..9595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9087..9106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9766..10557,10558..10626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10389..10408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10627..10731)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
       61 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      121 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      181 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      241 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      301 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      361 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      421 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      481 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      541 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      601 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      661 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      721 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      781 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
      841 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      901 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
      961 ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     1021 aggctccacc atgaggcaag tgctgtggtt gtgtaatgtc tgcgtaaccg cacgggaaac
     1081 ccgccaccac ctccacctcc ctgccatcct cgacaagatg cctgcccccg gcgccctcat
     1141 cctccttgcg gccgtctccg cctccggctg cctggcgtcc ccggcccacc ccgatggatt
     1201 cgccctgggc cgggctcctc tggctcctcc ctacgctgtg gtcctcattt cctgctccgg
     1261 cctgctggcc ttcatcttcc tcctcctcac ctgtctgtgc tgcaaacggg gcgatgtcgg
     1321 cttcaaggaa tttgagaacc ctgaagggga ggactgctcc ggggagtaca ctccccctgc
     1381 ggaggagacc tcctcctcac agtcgctgcc tgatgtctac attctcccgc tggctgaggt
     1441 ctccctgcca atgcctgccc cgcagccttc acactcagac atgaccaccc ccctgggcct
     1501 tagccggcag cacctgagct acctgcagga gattgggagt ggctggtttg ggaaggtgat
     1561 cctgggagag attttctccg actacacccc cgcccaggtg gtggtgaagg agctccgagc
     1621 cagcgcgggg cccctggagc aacgcaagtt catctcggaa gcacagccgt acaggagcct
     1681 gcagcacccc aatgtcctcc agtgcctggg tctgtgcgtg gagacgctgc cgtttctgct
     1741 gattatggag ttctgtcaac tgggggacct gaagcgttac ctccgagccc agcggccccc
     1801 cgagggcctg tcccctgagc taccccctcg agacctgcgg acgctgcaga ggatgggcct
     1861 ggagatcgcc cgcgggctgg cgcacctgca ttcccacaac tacgtgcaca gcgacctggc
     1921 cctgcgcaac tgcctgctga cctctgacct gaccgtgcgc atcggagact acgggctggc
     1981 ccacagcaac tacaaggagg actactacct gaccccagag cgcctgtgga tcccactgcg
     2041 ctgggcggcg cccgagctcc tcggggagct ccacgggacc ttcatggtgg tggaccagag
     2101 ccgcgagagc aacatctggt ccctgggggt gaccctgtgg gagctgtttg agtttggggc
     2161 ccagccctac cgccacctgt cagacgagga ggtcctcgcc ttcgtggtcc gccagcagca
     2221 tgtgaagctg gcccggccga ggctcaagct gccttacgcg gactactggt atgacattct
     2281 tcagtcctgc tggcggccac ctgcccagcg cccttcagcc tctgatctcc aattgcagct
     2341 cacctacttg ctctccgagc ggcctccccg gcccccaccg ccgccacccc caccccgaga
     2401 cggtcccttc ccctggccct ggccccctgc acacagtgcg ccccgcccgg ggaccctctc
     2461 ctcaccgttc cccctactgg atggcttccc tggagccgac cccgacgatg tgctcacggt
     2521 caccgagagt agccgcggcc tcaacctcga gtgcctgtgg gagaaggccc ggcgtggggc
     2581 cggccggggt gggggggcac ctgcctggca gccggcgtcg gcccccccgg ccccccacgc
     2641 caacccctcc aaccctttct acgaggcgct gtccacgccc agcgtgctgc ctgtcatcag
     2701 cgcccgcagc ccctccgtga gcagcgagta ctacatccgc ttggaggagc acggctcccc
     2761 tcctgagccc ctcttcccca acgactggga ccccctggac ccaggagtgc ccgcccctca
     2821 ggccccccag gccccctccg aggtccccca gctggtgtcc gagacctggg cctcccccct
     2881 cttccctgcg ccccggccct tcccagccca gtcctcagcg tcaggcagct tcctgctgag
     2941 cggctgggac cccgagggcc ggggcgccgg ggagaccctg gcgggagacc ctgccgaggt
     3001 cttgggggag cgggggaccg ccccgtgggt ggaagaagaa gaggaggagg aggagggcag
     3061 ctccccaggg gaagacagca gcagccttgg aggtggccca agccgccggg gtcccctacc
     3121 ctgtcccctg tgcagccgcg agggggcctg ctcctgcctg ccactggagc ggggggacgc
     3181 cgtagcaggc tggggaggcc accctgctct tggctgcccc cacccccccg aggacgactc
     3241 ctcgctgcgg gcagagcggg gctccctggc cgacttgccc atggcccccc ccgcctcggc
     3301 cccccccgag tttctggacc ccctcatggg ggcggcggcg ccccagtacc ccgggcgggg
     3361 gccacctccc gctccccccc ccccgccgcc acctcctcgg gcccccgcgg acccggccgc
     3421 gtcccccgac cccccttcgg ccgtggccag tcccggttca ggcctctcgt cgccgggccc
     3481 caagccgggg gacagcggct acgagaccga gacccctttt tccccagagg gagccttccc
     3541 aggtgggggg gcggccgagg aggaaggggt ccctcggccg cgggctcccc ccgagccacc
     3601 cgacccagga gcgccccggc cacctccaga cccgggtccg ctcccactcc cggggccccg
     3661 ggagaagccg accttcgtgg ttcaagtgag cacggaacag ctgctgatgt ccctgcggga
     3721 ggatgtgaca aggaacctcc tgggggagaa gggggcgaca gcccgggaga caggacccag
     3781 gaaggcgggg agaggccccg ggaacagaga gaaagtcccg ggcctgaaca gggacccgac
     3841 agtcctgggc aacgggaaac aagccccaag cctgagcctc ccagtgaacg gggtgacagt
     3901 gctggagaac ggggaccaga gagccccagg catcgaggag aaggcggcgg agaatggggc
     3961 cctggggtcc cccgagagag aagagaaagt gctggagaat ggggagctga cacccccaag
     4021 gagggaggag aaagcgctgg agaatgggga gctgaggtcc ccagaggccg gggagaaggt
     4081 gctggtgaat gggggcctga cacccccaaa gagcgaggac aaggtgtcag agaatggggg
     4141 cctgagattc cccaggaaca cggagaggcc accagagact gggccttgga gagccccagg
     4201 gccctgggag aagacgcccg agagttgggg tccagccccc acgatcgggg agccagcccc
     4261 agagacctct ctggagagag cccctgcacc cagcgcagtg gtctcctccc ggaacggcgg
     4321 ggagacagcc cctggccccc ttggcccagc ccccaagaac gggacgctgg aacccgggac
     4381 cgagaggaga gcccccgaga ctgggggggc gccgagagcc ccaggggctg ggaggctgga
     4441 cctcgggagt gggggccgag ccccagtggg cacggggacg gcccccggcg gcggccccgg
     4501 aagcggcgtg gacgcaaagg ccggatgggt agacaacacg aggccgcagc caccgccgcc
     4561 accgctgcca ccgccaccgg aggcacagcc gaggaggctg gagccagcgc ccccgagagc
     4621 caggccggag gtggcccccg agggagagcc cggggcccca gacagcaggg ccggcggaga
     4681 cacggcactc agcggagacg gggacccccc caagcccgag aggaagggcc ccgagatgcc
     4741 acgactattc ttggacttgg gaccccctca ggggaacagc gagcagatca aagccaggct
     4801 ctcccggctc tcgctggcgc tgccgccgct cacgctcacg ccattcccgg ggccgggccc
     4861 gcggcggccc ccgtgggagg gcgcggacgc cggggcggct ggcggggagg ccggcggggc
     4921 gggagcgccg gggccggcgg aggaggacgg ggaggacgag gacgaggacg aggaggagga
     4981 cgaggaggcg gcggcgccgg gcgcggcggc ggggccgcgg ggccccggga gggcgcgagc
     5041 agccccggtg cccgtcgtgg tgagcagcgc cgacgcggac gcggcccgcc cgctgcgggg
     5101 gctgctcaag tctccgcgcg gggccgacga gccagaggac agcgagctgg agaggaagcg
     5161 caagatggtc tccttccacg gggacgtgac cgtctacctc ttcgaccagg agacgccaac
     5221 caacgagctg agcgtccagg ccccccccga gggggacacg gacccgtcaa cgcctccagc
     5281 gcccccgaca cctccccacc ccgccacccc cggagatggg tttcccagca acgacagcgg
     5341 ctttggaggc agtttcgagt gggcggagga tttccccctc ctcccccctc caggcccccc
     5401 gctgtgcttc tcccgcttct ccgtctcgcc tgcgctggag accccggggc cacccgcccg
     5461 ggcccccgac gcccggcccg caggccccgt ggagaatgac ccagctttct tgtacaaagt
     5521 ggttgatatc aaggccgccg acatcaccag cctgtacaag aaagtggggt ggagccatcc
     5581 ccagttcgag aagggcggag gatctggcgg cggaagcggc ggaggaagct ggtcccaccc
     5641 tcagtttgag aaaggcaccg agctgggcag caccatggcc agctacccct acgacgtgcc
     5701 cgactacgcc aaggacaaca ccgtgcccct gaagctgatc gccctgctgg ccaacggcga
     5761 gttccactct ggcgagcagc tgggcgagac actgggcatg agcagagccg ccatcaacaa
     5821 gcacatccag accctgcggg actggggcgt ggacgtgttc acagtgcccg gcaagggcta
     5881 cagcctgccc gagcccatcc agctgctgaa cgccaagcag atcctgggcc agctggatgg
     5941 cggatctgtg gccgtgctgc ccgtgatcga cagcaccaac cagtatctgc tcgaccggat
     6001 cggcgagctg aagtccggcg acgcctgtat cgccgagtac cagcaggccg gcagaggcgg
     6061 caggggcaga aagtggttca gccccttcgg cgccaacctg tacctgagca tgttctggag
     6121 actggaacag ggcccagccg ccgctattgg cctgagcctg gtcatcggca tcgtgatggc
     6181 cgaggtgctg cggaagctgg gcgccgacaa agtgcgcgtg aagtggccca acgacctgta
     6241 tctgcaggac cggaagctgg ccggcatcct ggtggaactg accggcaaaa caggcgacgc
     6301 cgcccagatc gtgatcggcg ctggcatcaa catggccatg cggcgggtgg aagagagcgt
     6361 ggtcaaccag ggctggatca ccctgcagga agccggcatc aacctggacc ggaacaccct
     6421 ggccgccatg ctgatcagag agctgagagc cgccctggaa ctgttcgagc aggaaggcct
     6481 ggccccctac ctgagcagat gggagaagct ggacaacttc atcaaccggc ccgtgaaact
     6541 gatcatcggc gacaaagaga tcttcggcat cagccggggc atcgacaagc agggggccct
     6601 gctcctggaa caggacggca tcatcaagcc ctggatgggc ggcgagatca gcctgcggag
     6661 cgccgagaag tgatgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     6721 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6781 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6841 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6901 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6961 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7201 tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga
     7261 aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     7321 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     7381 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     7441 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     7501 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     7561 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     7621 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     7681 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     7741 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     7801 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     7861 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     7921 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     7981 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     8041 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     8101 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     8161 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     8221 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     8281 cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt
     8341 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8401 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8581 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8641 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8701 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8761 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10081 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10381 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10621 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10681 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10741 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgc
//
