LOCUS       sequence-372763-        9209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 2xNLS-NLP-cMyc LbaCas12a
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1321..1512
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1506..1528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1614..1753
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1669..1688
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1768..1785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1939..2527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2019..2038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2698..3489,3490..3558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3321..3340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3559..3663)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3689..4144)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3826..3847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4038..4057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(4181..4228)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4224..4242
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4295..4312)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4319..4345)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(4346..4426)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(4433..4480)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4481..8164)
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     RBS             8172..8194
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8209..8233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8233..8252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8234..8252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8336..8355)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        8561..8638
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(8639..9209,1..512)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8658..8677)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
       61 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      121 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      181 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      241 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      301 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
      361 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
      421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc
      541 actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
      601 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
      661 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
      721 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
      781 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
      841 ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
      901 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
      961 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     1021 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     1081 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     1141 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     1201 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     1261 tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca
     1321 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     1381 acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     1441 cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     1501 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1561 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     1621 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     1681 agattgtact gagagtgcac cattgcggtg tgaaataccg cacagatgcg taaggagaaa
     1741 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2341 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3001 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3661 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     3721 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3781 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3841 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3901 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3961 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4021 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4081 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4141 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
     4201 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
     4261 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagat
     4321 ccagtttcac cctcttagct gccggggcat agtcggggac atcatatggg tatgcataat
     4381 caggcacgtc gtagggataa gcgtaatctg gaacatcgta tgggtaggat ccctttttct
     4441 tttttgcctg gccggccttt ttcgtggccg ccggcctttt gtgcttcacg ctggtctggg
     4501 cgtactccag ccactccttg ttagagatgg cgatcttcac cttatccagc ttctcgtcct
     4561 cggccttctt gaactggccg atggcccaca gcacctttct ggcgatgtta taggcgccat
     4621 tggcgtcggc gttctttggc aggatggcat tctcctgggc ctcatagttc cggctatcgt
     4681 agaagatgcc gtcggagttc ttcacagggc tgatcagaaa atccacgtcg gtgcggcctg
     4741 tgatgctgtt ccgcatctgc agcatcaggc tcatcagggc cataaagcta gagtagaagg
     4801 ccttgtcgga ctgctcgcac agcagggctc tgatatcgcc ctgctgataa ttgatgccgt
     4861 acttgttgaa cagctcctta taggcgctgg tcaggcacac ctcctcccag tcgaacacgt
     4921 tgttcttctt aggattccgg aagattctga tccggttgcc gtaggagtac agcttccact
     4981 tcttgatgta atcggcgtct gtgcgagaga agttcttata gtccagggca aactcgaaca
     5041 gatcctcctc gggcacgtac atgatcctgt caaaggagct gatgaacttc ttggaatcgg
     5101 cgatgctggt atacttggtt ttcagcaggt tcacaaagcc ggtagatgga tcgatcttgg
     5161 atgtcagcca ggcagggatg taaaagatga agccgttctg ggtagacatg gacttaaagc
     5221 tctcgaactt attggtgatc tgatagccct tcagggcgcc gcctgttgca caaggattag
     5281 acttcttgtc caccatgtag ttcagcttat cgatcagcat cttctcgaac ttctgataca
     5341 cctgcttctc caccttcacg cggctattct taaagccaga gttcaggtcc tccagggcga
     5401 tcacggcatc gtacttctcc accagctcgc agatcttgtg caccacctga gagatatagc
     5461 cggccttcag ctccttgata ttctcgatgg aggtccagtt ctggcgggcc tcgaacctct
     5521 ccttctcctt cttgtccagc agagagtggt aatctgtctt gatcctgatg ccgttgaagt
     5581 tgttgatgat ctcgttcagg gaatactgct ccacgatgtt gcccttgccg tccaccacca
     5641 cgatatacag cagattgcgc tcgcccctat cgatgccgat cacatagggg ttatcgtcgt
     5701 gcttcagcag cacgcgcacc tctgtattga tcttgaagat gttcttgggg cacttattga
     5761 tggcgattgg gatgtgcagc tcgtactggt cctcagaaaa cctcttatcc ttatacacgt
     5821 cgtaggacag ggttgtggtt ttcttgggat tatctggatt cttgttggcg ataggggagt
     5881 tggctgggtg caccaccagc tcctccttct tcagggaggc gcgcctcatg aacagctctg
     5941 ctcctccgct cagcctgatc tgtccgtgat tgttctcgtc aaacagcagc ttgaagtaca
     6001 tggtgtgcag attgggtgtg ccgtgagact tatcggaaaa gtccttgtta tagatctgga
     6061 acatatacag cttgccctcc tccaccagct tatccacctc cttcttgctg gcagactcga
     6121 agctcacctt atagccctgc tcctccacct ctctgtaaaa gccggcgatg tccttatact
     6181 tctctgtctc agaaaagttg aaatcgtagg cattggacca ctttggatac cgggagatgc
     6241 tatccttaaa gaagtcgatc agcttgtgac agtcattcag gttaaacata tcgcccttct
     6301 tgaatgtgcc attcttgtag atcttctgga tgtcctcgct ggggttatag taggccatcc
     6361 acttcttaga aaagaacacc tttggcagca tcttattagg gccgggcagc agcttatagt
     6421 tgatcttctc gtaattgccg ttcacatcgt ccttgtcgat cttctgcagg cacttggcgt
     6481 acttcttatc catgatggcc agatagtact tggagccgta tctcaggatg gtggcccgat
     6541 agtctgtctc cttatccttg tcccagccgc ccatgaactg agggttctga aaatacagct
     6601 tgaacttatc cttagagtag ggcttctggg tcacataatt gcggatggca tcgtagatgt
     6661 ggtccacctt cagcaggatg tcgtaggcca gcacaaaatc gccatagaag gactcgtccc
     6721 tgtttgtctc cttgccctcg ccaaagaagg ccttgatgta attctcgaag ctcttcacag
     6781 aatccagcag gtccttcatg atggccacca cggcgtcgtt cttcttcagg ctcttctcca
     6841 gcacaaaatc ggcgtcgaac agcttctcag aggagccata caccttgtag atctcatcca
     6901 ccttctggat gatgatctcc ttcagcttct ccaccacaga cagatcggcg tcggcgtact
     6961 cctgcagctg ctccagagaa aaggagccga tcttcttgaa ggactttctc cgatcgtcct
     7021 cgtacttctc ggtcaccacg gccttcttct tcaggtggat atcgtcatac tcggcattcc
     7081 acttgtcccg gatcacgttc cactcgccga agatatcctt ggagattgtg ctgatggcgg
     7141 ggccgttctt cacaaagatg ccggcgctag agtactcgtc aaaattcttg aacagcttct
     7201 ccagcttctt gatggagctg aagatctcgc tgttcttgtt cagggtgttt ctaaacacct
     7261 ccagcacctc ctcatcggat gtatagccct cgccgtagaa gctcagagac tcccgatcgc
     7321 tcagcacctg cttatacagt ggcttaaact taggcagctt ctgcttggtt ttctgattat
     7381 acaggttgat gtactcgttc aggcccttga tcttctcgcc gctctcggtc acgaagccgc
     7441 cgatgatggc gttatacacg tcgatgccct cctgtgtcag cacaaagtta aagaactcgc
     7501 cctcaaagaa atcctccaca tcatagtcgc tgttcaggat cttctccttg atctcctgca
     7561 cctcgtgctt atcaaagatg gcgtccacct tctcgaagat gtccatatta gagatgtagc
     7621 gggtcagatt ctcgttgata cacctgaagg cgatggatgt gctcttggcc tcctcggaaa
     7681 acatattctc tctgttatca aagaagccgg tgaaggctgt ggtaaagcca ttgaagctgt
     7741 tcaccagggc gatctcgtcc ttatcgtcca ggaactctgg caggattgtc tcgatgatat
     7801 ccttcttaaa cagggacttg tagccctcgt tgcccttgaa ggccttggcg atctccttcc
     7861 gcagattgat ctccaggttc tccagctcct tattctcctt ctcggttctg gttttcttcc
     7921 ggaacaggct gatgtaattg ttcagattct tcagcttgat gctgtgcagc acgtcgttga
     7981 taaaagacag atagtagcga tccagcagct tcttcacgcc cttataatcc tcggctctct
     8041 tctcgtcctc caccagcagc cgcttattgt cgatgttctc ctgggtcttg cccacaggga
     8101 tggccttgaa cctcagggtc ttagacaggg agtagcagtt tgtaaacttc tccagcttgc
     8161 tcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc
     8221 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc
     8281 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     8341 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     8401 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     8461 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     8521 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa
     8581 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt
     8641 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     8701 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     8761 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     8821 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     8881 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     8941 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     9001 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     9061 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     9121 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     9181 aatcgcgctg ttagcgggcc cattaagtt
//
