LOCUS       sequence-37280-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pVimentin-PSmOrange
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..842)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(212..235)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cataacagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
       61 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga
      121 gtcgcggccg cttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
      181 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg
      241 ccgacgatgt aggcgccggg cagctgcacg gacttcttgg ccttgtaggt ggtcttgacc
      301 tcggaggtgt agtggccgcc gtccttcagc ttcagcctca tcctgatctc gcccttcagg
      361 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
      421 atcacggggc cgtcggaggg gaagttggtg ccgcgcatct tcaccttgta gatgaactcg
      481 ccgtcctgca gtgaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gtagttcatc
      541 acgcgctccc acttgaagcc ctcggggaag gacagcttga agtagtcggg gatgtcggcg
      601 gggtgcttca cgtaggcctt ggagccgtag gtgaagagag gggacaggat gtcccaggcg
      661 aagggcaggg ggccgccctt ggtcaccttc agcttagcgg tctgaaagcc ctcgtagggg
      721 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggtgcc ctccatgcgc
      781 accttgaagc gcatgaactc cttgatgatg gccatattat tctcctcgcc cttgctcacc
      841 atggtggcga ccggtggatc ccctca
//
