LOCUS       sequence-37287-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pPaxillin-PSmOrange
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..846)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(216..239)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      843..852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgcataac agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 tagagtcgcg gccgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
      181 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      241 gatgccgacg atgtaggcgc cgggcagctg cacggacttc ttggccttgt aggtggtctt
      301 gacctcggag gtgtagtggc cgccgtcctt cagcttcagc ctcatcctga tctcgccctt
      361 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
      421 ctgcatcacg gggccgtcgg aggggaagtt ggtgccgcgc atcttcacct tgtagatgaa
      481 ctcgccgtcc tgcagtgagg agtcctgggt cacggtcacc acgccgccgt cctcgtagtt
      541 catcacgcgc tcccacttga agccctcggg gaaggacagc ttgaagtagt cggggatgtc
      601 ggcggggtgc ttcacgtagg ccttggagcc gtaggtgaag agaggggaca ggatgtccca
      661 ggcgaagggc agggggccgc ccttggtcac cttcagctta gcggtctgaa agccctcgta
      721 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat
      781 gcgcaccttg aagcgcatga actccttgat gatggccata ttattctcct cgcccttgct
      841 caccatggtg gcgacc
//
