LOCUS       sequence-372996-        2351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N-term V5 tag, frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-V5 (w71-3)
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(171..212)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(224..323)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(342..365)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(373..400)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(532..575)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(743..1331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(823..842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1421..2230)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2144..2163
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2222..2241
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
       61 atgctttctt ataatgccaa ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg
      121 ccgcgaattc ggtaccggat ccagtcgact gaattggttc ccatggatcc cgtagaatcg
      181 agaccgagga gagggttagg gataggctta ccttccatgg tggagcctgc ttttttgtac
      241 aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      301 agtcaaaata aaatcattat ttggggcccg agcttaagta actaactaac aggaagagtt
      361 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      421 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      481 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      541 aaggctcagt cggaagactg ggcctttcgt tttatttgat gcctggcagt tccctactct
      601 cgcttagtag ttagacgtcc ccgagatcca tgctagcggt aatacggtta tccacagaat
      661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgggg
     1381 cccaatctga ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     1441 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1501 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1561 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1621 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1681 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1741 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1801 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1861 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1921 ctgtttttcc ggggatcgca gtggtgagta accatgcatc accaggagta cggataaaat
     1981 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2041 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2101 tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     2161 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa
     2221 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     2281 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct
     2341 gcagctggat g
//
