LOCUS       sequence-373148-        3279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of excitatory 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CaMKII-somBiPOLES-eGFP
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(958..960,961..1671)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(958..960,961..1671)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1000..1021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1261..1280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1608..1629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2032..3075
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccgtg
      961 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1021 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1081 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1141 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1201 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1261 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1321 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1381 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1441 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1501 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1561 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1621 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaccggtgca
     1681 gccctgcagg agaagaagtc atgcagccag cgcatggccg aattccggca atactgttgg
     1741 aacccggaca ctgggcagat gctgggccgc accccagccc ggtgggtgtg gatcagcctg
     1801 tactatgcag ctttctacgt ggtcatgact gggctctttg ccttgtgcat ctatgtgctg
     1861 atgcagacca ttgatcccta cacccccgac taccaggacc agttaaagtc accgggggta
     1921 accttgagac cggatgtgta tggggaaaga gggctgcaga tttcctacaa catctctgaa
     1981 aacagctcta gacaggccca gatcaccgga cgtccggagg gagctgtaca agagctgatc
     2041 agcagcgcca ccagatctct gtttgccgcc ggaggcatca acccttggcc taacccctac
     2101 caccacgagg acatgggctg tggaggaatg acacctacag gcgagtgctt cagcaccgag
     2161 tggtggtgtg acccttctta cggactgagc gacgccggat acggatattg cttcgtggag
     2221 gccacaggcg gctacctggt cgtgggagtg gagaagaagc aggcttggct gcacagcaga
     2281 ggcacaccag gagaaaagat cggcgcccag gtctgccagt ggattgcttt cagcatcgcc
     2341 atcgccctgc tgacattcta cggcttcagc gcctggaagg ccacttgcgg ttgggaggag
     2401 gtctacgtct gttgcgtcga ggtgctgttc gtgaccctgg agatcttcaa ggagttcagc
     2461 agccccgcca cagtgtacct gtctaccggc aaccacgcct attgcctgcg ctacttcgag
     2521 tggctgctgt cttgccccgt gatcctgatc aagctgagca acctgagcgg cctgaagaac
     2581 gactacagca agcggaccat gggcctgatc gtgtcttgcg tgggaatgat cgtgttcggc
     2641 atggccgcag gactggctac cgattggctc aagtggctgc tgtatatcgt gtcttgcatc
     2701 tacggcggct acatgtactt ccaggccgcc aagtgctacg tggaagccaa ccacagcgtg
     2761 cctaaaggcc attgccgcat ggtcgtgaag ctgatggcct acgcttactt cgcctcttgg
     2821 ggcagctacc caatcctctg ggcagtggga ccagaaggac tgctgaagct gagcccttac
     2881 gccaacagca tcggccacag catctgcgac atcatcgcca aggagttttg gaccttcctg
     2941 gcccaccacc tgaggatcaa gatccacgag cacatcctga tccacggcga catccggaag
     3001 accaccaaga tggagatcgg aggcgaggag gtggaagtgg aagagttcgt ggaggaggag
     3061 gacgaggaca cagtgctcga gcagagccag cccatcctga acaccaagga gatggccccc
     3121 cagagcaagc cccccgagga gctggagatg agcagcatgc ccagccccgt ggcccccctg
     3181 cccgcccgca ccgagggcgt gatcgacatg cgcagcatga gcagcatcga cagcttcatc
     3241 agctgcgcca ccgacttccc cgaggccacc cgcttctga
//
