LOCUS       sequence-373243-       10926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-GFP-AnillinFL
FEATURES             Location/Qualifiers
     source          1..10926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2616..2928
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2847..2867
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2991..3007
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3049..3082)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3162..3465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3466..3669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3708..3717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3714..3716,3717..3719,3720..4430)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3714..3716,3717..3719,3720..4430)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3759..3780)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4020..4039)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4367..4388
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7728..7761)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7817..8403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7870..7890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8288..8299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8406..8422)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8932..9112
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9174..9762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9254..9273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9933..10724,10725..10793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10556..10575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10794..10898)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgcgct ctagagatcc gacgcgccat ctctaggccc
     2641 gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg ccccggttaa
     2701 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
     2761 ctgttctttt taatactagc tacattttac atgataggct tggatttcta taagagatac
     2821 aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta actgtaaagt
     2881 aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttc ggcgatgcct
     2941 ctttgaataa attcaagaga tttattcaaa gaggcatcgc cattttttct cgaggtcgac
     3001 ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat aacttcgtat
     3061 agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc atagtaatca
     3121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3661 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
     3721 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3781 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3841 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3901 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3961 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4021 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4081 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4141 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4201 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4261 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4321 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4381 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ccgcggatgg
     4441 atccgtttac ggagaaactg ctggagcgaa cccgtgccag gcgagagaat cttcagagaa
     4501 aaatggctga gaggcccaca gcagctccaa ggtctatgac tcatgctaag cgagctagac
     4561 agccactttc agaagcaagt aaccagcagc ccctctctgg tggtgaagag aaatcttgta
     4621 caaaaccatc gccatcaaaa aaacgctgtt ctgacaacac tgaagtagaa gtttctaact
     4681 tggaaaataa acaaccagtt gagtcgacat ctgcaaaatc ttgttctcca agtcctgtgt
     4741 ctcctcaggt gcagccacaa gcagcagata ccatcagtga ttctgttgct gtcccggcat
     4801 cactgctggg catgaggaga gggctgaact caagattgga agcaactgca gcctcctcag
     4861 ttaaaacacg tatgcaaaaa cttgcagagc aacggcgccg ttgggataat gatgatatga
     4921 cagatgacat tcctgaaagc tcactcttct caccaatgcc atcagaggaa aaggctgctt
     4981 cccctcccag acctctgctt tcaaatgcct cggcaactcc agttggcaga aggggccgtc
     5041 tggccaatct tgctgcaact atttgctcct gggaagatga tgtaaatcac tcatttgcaa
     5101 aacaaaacag tgtacaagaa cagcctggta ccgcttgttt atccaaattt tcctctgcaa
     5161 gtggagcatc tgctaggatc aatagcagca gtgttaagca ggaagctaca ttctgttccc
     5221 aaagggatgg cgacgcaagc ttaaacaagg ccctatcctc aagtgctgct gatgcgtctt
     5281 tggttaatgc ctcaatttcc agctctgtga aagctacttc tccagtgaaa tctactacat
     5341 ctatcactga tgctaaaagt tgtgagggac aaaatcctga gctacttcca aaaactccta
     5401 ttagtcctct gaaaacgggg gtatcgaaac caattgtgaa gtcaacttta tcccagacag
     5461 ttccatccaa gggagaatta agtagagaaa tttgtctgca atctcaatct aaagacaaat
     5521 ctacgacacc aggaggaaca ggaattaagc ctttcctgga acgctttgga gagcgttgtc
     5581 aagaacatag caaagaaagt ccagctcgta gcacacccca cagaaccccc attattactc
     5641 caaatacaaa ggccatccaa gaaaaattat tcaagcaaga cacatcttca tctactaccc
     5701 atttagcaca acagctcaag caggaacgtc aaaaagaact agcatgtctt cgtggccgat
     5761 ttgacaaggg caatatatgg agtgcagaaa aaggcggaaa ctcaaaaagc aaacaactag
     5821 aaaccaaaca ggaaactcac tgtcagagca ctcccctcaa aaaacaccaa ggtgtttcaa
     5881 aaactcagtc acttccagta acagaaaagg tgaccgaaaa ccagatacca gccaaaaatt
     5941 ctagtacaga acctaaagaa gtgatacgtg aaattgagat gagtgtggat gatgatgata
     6001 tcaatagttc gaaagtaatt aatgacctct tcagtgatgt cctagaggaa ggtgaactag
     6061 atatggagaa gagccaagag gagatggatc aagcattagc agaaagcagc gaagaacagg
     6121 aagatgcact gaatatctcc tcaatgtctt tacttgcacc attggcacaa acagttggtg
     6181 tggtaagtcc agagagttta gtgtccacac ctagactgga attgaaagac accagcagaa
     6241 gtgatgaaag tccaaaacca ggaaaattcc aaagaactcg tgtccctcga gctgaatctg
     6301 gtgatagcct tggttctgaa gatcgtgatc ttctttacag cattgatgca tatagatctc
     6361 aaagattcaa agaaacagaa cgtccatcaa taaagcaggt gattgttcgg aaggaagatg
     6421 ttacttcaaa actggatgaa aaaaataatg cctttccttg tcaagttaat atcaaacaga
     6481 aaatgcagga actcaataac gaaataaata tgcaacagac agtgatctat caagctagcc
     6541 aggctcttaa ctgctgtgtt gatgaagaac atggaaaagg gtccctagaa gaagctgaag
     6601 cagaaagact tcttctaatt gcaactggga agagaacact tttgattgat gaattgaata
     6661 aattgaagaa cgaaggacct cagaggaaga ataaggctag tccccaaagt gaatttatgc
     6721 catccaaagg atcagttact ttgtcagaaa tccgcttgcc tctaaaagca gattttgtct
     6781 gcagtacggt tcagaaacca gatgcagcaa attactatta cttaattata ctaaaagcag
     6841 gagctgaaaa tatggtagcc acaccattag caagtacttc aaactctctt aacggtgatg
     6901 ctctgacatt cactactaca tttactctgc aagatgtatc caatgacttt gaaataaata
     6961 ttgaagttta cagcttggtg caaaagaaag atccctcagg ccttgataag aagaaaaaaa
     7021 catccaagtc caaggctatt actccaaagc gactcctcac atctataacc acaaaaagca
     7081 acattcattc ttcagtcatg gccagtccag gaggtcttag tgctgtgcga accagcaact
     7141 tcgcccttgt tggatcttac acattatcat tgtcttcagt aggaaatact aagtttgttc
     7201 tggacaaggt ccccttttta tcttctttgg aaggtcatat ttatttaaaa ataaaatgtc
     7261 aagtgaattc cagtgttgaa gaaagaggtt ttctaaccat atttgaagat gttagtggtt
     7321 ttggtgcctg gcatcgaaga tggtgtgttc tttctggaaa ctgtatatct tattggactt
     7381 atccagatga tgagaaacgc aagaatccca taggaaggat aaatctggct aattgtacca
     7441 gtcgtcagat agaaccagcc aacagagaat tttgtgcaag acgcaacact tttgaattaa
     7501 ttactgtccg accacaaaga gaagatgacc gagagactct tgtcagccaa tgcagggaca
     7561 cactctgtgt taccaagaac tggctgtctg cagatactaa agaagagcgg gatctctgga
     7621 tgcaaaaact caatcaagtt cttgttgata ttcgcctctg gcaacctgat gcttgctaca
     7681 aacctattgg aaagccttaa cctgcaggca attcgtcgag ggacctaata acttcgtata
     7741 gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga
     7801 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7921 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7981 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8041 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8101 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8161 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8221 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8281 cgtcccttcg gccctcaatc cagcggacct tccttcccgg cctgctgccg gctctgcggc
     8341 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8401 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     8461 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     8521 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     8581 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     8641 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     8701 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     8761 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     8821 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     8881 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     8941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
     9121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10921 cctgac
//
