LOCUS       sequence-373301-        2556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop 
            Apt-eIF4G into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: synIRES_RC25
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1490..2281,2282..2350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2113..2132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2351..2455)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgtg cggccgctcg gtctcatatc ttaaaacagc
      721 ggatgggtac cccaccatcc gacccactgg gtgtagtact ctggtacttc gtacctttgt
      781 acgcctgttc ttcccattgt acccttcctg aacttccaac ccaagtaacg ttagaagctc
      841 aacatttagt acaacaggaa gcaccacatc cagtggtgtt tagtacaagc acttctgttt
      901 ccccggagcg aggtataggc tgtacccact gccaaaaacc tttaaccgtt atccgccaac
      961 caactacgta aaagctagta gtattatgtt tttaactagg cgttcgatca ggtggatttc
     1021 ccctccacta gtttggtcga tgaggctagg aattccccac gggtgaccgt gtcctagcct
     1081 gcgtggcggc caacccagcc cactcactat ttgttttcgc gcccagttgc aaaaagtgtc
     1141 ggggctggga cgccttttta tagacatggt gtgaagactc gcatgtgctt ggttgtgatt
     1201 cctccggccc ctgaatgcgg ctaaccttaa ccctggagcc ttgtgtcaca aaccagtgat
     1261 gataaggtcg taatgagcaa ttccgggacg ggaccgacta ctttgggtgt ccgtgtttct
     1321 tatttttctt attattgtct tatggtcaca gcatatatat aacatatact gtgatcatgg
     1381 tgagaccgcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1621 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2461 ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact
     2521 gagcgtcaga ccccgtagaa aagatcaaag gatctt
//
