LOCUS       sequence-373420-       10673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for lentiviral pegRNA library construction with hMLH1dn and
            Puro marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH
  TITLE     Prediction of efficiencies for diverse prime editing systems in 
            multiple cell types.
  JOURNAL   Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 
            10.1016/j.cell.2023.03.034.
  PUBMED    37119812
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034."
COMMENT     Sequence Label: pLenti_gRNA -hMLHdn-Puro
FEATURES             Location/Qualifiers
     source          1..10673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1715..1963
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1715..1735
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1886..1905
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2123..2240
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2379..3557
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2610..3548
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3505..3525
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5856..5912
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5913..6512
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5913..5932)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6409..6429
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6528..7116
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6581..6601)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6999..7010)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7188..7421
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7499..7620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7536..7555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7590..7609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7660..7795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7815..7834)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7816..7834)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7844..7861)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7844..7860)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7853..7875)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8002..8457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8089..8108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8299..8320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8483..8587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8588..8656,8657..9448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8806..8825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9619..10207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10108..10127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10361..10378
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10495..10516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10531..10536,10537..10554,10555..10561)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10569..10585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10574..10596
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10593..10609
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10593..10609
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10628..10648
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10630..10648
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc
     1741 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     1801 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     1861 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     1921 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg ttgactatcg
     1981 tctcgctact ctaccacttg tacttcagcg gtcagcttac tcgacttaac gtgcacgtga
     2041 cacgttctag accgtacatg cttacatggg atgaagcttg gcgtaactag atcttgagac
     2101 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2161 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2221 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2281 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2341 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2401 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2461 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2521 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2581 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2641 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2701 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2761 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2821 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2881 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2941 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3001 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3061 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3121 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3181 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3241 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3301 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3361 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3421 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3481 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3541 catttcaggt gtcgtgacgt acgggatcct ctagagccac catgagcttc gttgctggag
     3601 tcatccggag actggacgag acagtggtga acagaattgc cgccggcgag gtgatccaga
     3661 gacctgccaa tgcaatcaag gagatgatcg agaactgtct ggacgccaag tccacaagca
     3721 ttcaggtgat cgtgaaggag ggcggactga agctgatcca gatccaagac aacggcacag
     3781 gcatcagaaa ggaagatctg gacatcgtgt gtgaacggtt caccacatct aagctgcagt
     3841 cttttgagga tctggcctct atcagtacct acggcttcag aggcgaggcc ctggccagca
     3901 tcagccacgt ggcccatgtg accatcacca ccaaaaccgc cgacggcaaa tgcgcttatc
     3961 gcgctagcta cagcgacggc aagctgaaag ccccgccaaa gccttgcgcc ggcaaccagg
     4021 gtacacagat aacagtggag gatctgttct acaacatcgc cacccggaga aaggccctga
     4081 aaaatcccag cgaggagtac ggcaagatcc tggaagtcgt cggcagatac tccgtgcaca
     4141 acgccggaat cagctttagc gtaaagaagc agggagaaac cgtggccgat gtgcgcaccc
     4201 tgccaaatgc cagcaccgtg gataacatca gaagcatttt cggaaatgcc gtgtccagag
     4261 aactgatcga gatcggctgc gaagataaga ccctggcttt taagatgaac ggctacatca
     4321 gcaacgccaa ttactctgtg aagaagtgca tctttcttct gttcatcaac cacagactgg
     4381 tggaaagcac cagcctgcgg aaagccatcg agacagtgta cgccgcctac ctgcctaaga
     4441 acacccaccc cttcctgtac ctgagcctcg agatcagccc tcagaacgtg gacgtcaatg
     4501 tgcatcctac aaagcacgag gtgcacttcc tgcacgagga aagcatcctg gaaagagtgc
     4561 agcagcacat tgagagcaag ctgctgggct ctaacagcag cagaatgtac ttcacacaga
     4621 ccctgctgcc tggcctggcc ggcccctcag gcgaaatggt taagtccaca acctctctga
     4681 cctcatccag caccagcggt tcttccgata aggtgtacgc ccaccagatg gtgcggaccg
     4741 actctcggga gcagaagctg gacgcctttc tgcaacctct gagcaaacct ctgagctctc
     4801 agcctcaggc catcgtgacc gaggacaaga cagatatctc ctccggccgt gccagacagc
     4861 aggacgaaga aatgctcgag ctgccagctc ctgccgaggt ggccgccaag aaccagagcc
     4921 tggagggaga taccacaaag ggcaccagcg aaatgagcga gaagcggggc cctacctcca
     4981 gcaaccccag aaaacggcac cgggaggaca gcgacgtgga aatggtggag gacgacagca
     5041 gaaaggaaat gacagccgct tgtaccccta gaagaagaat catcaacctg acctccgtgc
     5101 tgagcctgca ggaggagatc aacgagcagg gccacgaggt gctgagagag atgctgcaca
     5161 atcacagctt cgtgggctgc gtgaaccctc aatgggccct ggctcagcat caaacaaagc
     5221 tgtacctgct gaacaccacc aagctgagcg aagagctgtt ctaccagatc ctcatctacg
     5281 acttcgccaa cttcggcgtg ctacgcctga gcgagcccgc ccctctgttt gacctggcca
     5341 tgctggctct ggatagccca gaaagcggct ggacagaaga ggacggacct aaagaggggc
     5401 tggctgaata catcgtggag ttcctgaaga aaaaggccga gatgctggcc gactactttt
     5461 ctctggaaat cgacgaggaa ggcaacctga tcggcctgcc tctgctgatc gataactacg
     5521 tgcctcccct ggaaggcctg cccatcttca tcctgagact ggctacagag gtgaactggg
     5581 acgaggaaaa ggaatgcttc gagtctctga gcaaggagtg cgccatgttc tatagcatca
     5641 gaaaacagta catctctgaa gagagcactc tgtctggcca gcagagtgaa gtgcccggaa
     5701 gcatccccaa cagctggaag tggaccgtgg aacacatcgt gtacaaggcc ctgcggagcc
     5761 acattctccc tcctaagcac ttcaccgagg acggcaacat cctgcagctg gccaacctgc
     5821 ccgaccttta taaggttttc gctagcggca gcggcgccac caacttcagc ctgctgaagc
     5881 aggccggcga cgtggaggag aaccccggcc ccatgaccga gtacaagccc acggtgcgcc
     5941 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6001 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6061 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6121 gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6181 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6241 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag
     6301 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6361 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6421 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6481 cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg
     6541 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6601 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6661 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6721 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6781 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6841 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6901 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6961 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7021 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7081 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     7141 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7201 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7261 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7321 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7381 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     7441 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7621 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7741 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     7801 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7861 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7921 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7981 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8041 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8101 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8161 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8221 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8281 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8341 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8401 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     8461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8581 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8701 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8761 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8821 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8881 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8941 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9001 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9121 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9181 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9241 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9301 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9421 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9721 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10261 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10321 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10381 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10441 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    10501 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    10561 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    10621 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
