LOCUS       sequence-373425-        2931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding histone H2B flanked by Gateway 
            recombination sequences. No Stop sequence: to be recombined into a 
            destination vector with a C-terminal protein tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-L1-Kozak-HistoneH2B-L2
FEATURES             Location/Qualifiers
     source          1..2931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(126..714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(206..225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(861..1670)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1584..1603
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1662..1681
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1783..1799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1783..1799
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1804..1823
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1804..1822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1840..1939
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1941..2318)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     protein_bind    complement(2327..2426)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2442..2459)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2442..2458)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2451..2473)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2522..2608
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2700..2727
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2886..2903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
       61 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      541 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      721 cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact cacgttaagg
      781 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg ccagtgttac
      841 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      901 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      961 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     1021 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     1081 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     1141 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     1201 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     1261 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     1321 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca
     1381 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     1441 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     1501 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     1561 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     1621 atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt
     1681 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1741 gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac agctatgacc
     1801 atgtaatacg actcactata ggggatatca gctggatggc aaataatgat tttattttga
     1861 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact
     1921 ttgtacaaga aagctgggtt cttagcgctg gtgtacttgg tgatggcctt agtaccctcg
     1981 gacacggcgt gcttggccaa ctccccaggc agcagcaggc gcacggccgt ctggatctcc
     2041 ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg
     2101 cgctcgaaaa tgtcgttcac aaacgaattc atgatgccca tggccttgga cgaaatgccg
     2161 gtgtcagggt ggacctgctt cagaaccttg tacacataga tggaatagct ctccttgcgg
     2221 ctgcgcttgc gcttcttgcc gcctttcttc tgcgccttag tcaccgcctt cttggagccc
     2281 tttttcgggg cgggagcaga cttcgctggc tctggcattt tttctgagcc tgcttttttg
     2341 tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact
     2401 atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa
     2461 cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca
     2521 tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc
     2581 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
     2641 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
     2701 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta
     2761 cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2821 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc g
//
