LOCUS       sequence-373996-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TAOK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TAOK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8440..8464)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8466..8507
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8514..9284
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9290..9310)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9339..9927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9392..9412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9810..9821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9941..10440
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9976..9999)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10373..10392
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10461..11060
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10461..10480)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10957..10977
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11198..11431
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11503..11624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11540..11559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11594..11613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11664..11799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11819..11838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11820..11838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11848..11865)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11848..11864)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11857..11879)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12006..12434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12093..12112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12303..12324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatgccatc aactaacaga gcaggcagcc
     5461 tgaaggaccc tgaaattgca gagctcttct tcaaagaaga tccagagaag ctcttcacag
     5521 atctcagaga aattggccat ggaagctttg gagcagtgta ttttgcacga gatgtgcgta
     5581 ccaatgaagt ggtggccatc aagaaaatgt cttatagtgg aaagcagtct actgagaaat
     5641 ggcaggatat tattaaggaa gtcaagtttc tacaaagaat aaaacatccc aacagtatag
     5701 aatacaaagg ctgttattta cgtgaacaca cagcatggct tgtaatggaa tattgtttag
     5761 gatctgcttc ggatttacta gaagttcaca aaaagccatt acaagaagtg gaaatagcag
     5821 caattacaca tggtgctctt cagggattag cctacttaca ttctcatact atgattcata
     5881 gagatatcaa agcaggaaat atccttctga cagaaccagg ccaggtgaaa cttgctgact
     5941 ttggctctgc ttccatggca tcacctgcca attcctttgt gggaacgccg tattggatgg
     6001 ccccagaagt aattttagcc atggatgaag gacaatatga tggcaaagta gatgtgtggt
     6061 ctcttggaat aacatgtatt gaactagcgg aaaggaagcc tcctttattt aatatgaatg
     6121 caatgagtgc cttatatcac atagcccaaa atgaatcccc tacactacag tctaatgaat
     6181 ggtctgatta ttttcgcaac tttgtagatt cttgcctcca gaaaatccct caagatcgac
     6241 ctacatcaga ggaactttta aagcacatat ttgttcttcg ggagcgccct gaaaccgtgt
     6301 taatagatct cattcagagg acaaaggatg cagtaagaga gctggacaat ctgcagtatc
     6361 gaaagatgaa gaaactcctt ttccaggagg cacataatgg accagcagta gaagcacagg
     6421 aagaagaaga ggaacaagat catggtgttg gccggacagg aacagttaat agtgttggaa
     6481 gtaatcaatc cattcccagc atgtccatca gtgccagcag ccaaagcagt agtgttaaca
     6541 gtcttccaga tgtctcagat gacaagagtg agctagacat gatggaggga gaccacacag
     6601 tgatgtctaa cagttctgtt atccatttaa aaccagagga agaaaattac agagaagagg
     6661 gagatcctag aacaagagca tcagatccac aatctccacc ccaagtatct cgtcacaaat
     6721 cacactatcg taatcgagaa cactttgcta ctatacggac agcatcactg gttacgaggc
     6781 aaatgcaaga acatgagcag gactctgagc ttagagaaca aatgtctggc tataagcgaa
     6841 tgaggcgaca acatcaaaag caactgatga ctctggaaaa caagctaaag gctgagatgg
     6901 atgaacatcg cctcagatta gacaaagatc ttgaaactca gcgtaacaat tttgctgcag
     6961 aaatggagaa acttatcaag aaacaccagg ctgctatgga gaaagaggct aaagtgatgt
     7021 ccaatgaaga gaaaaaattt cagcaacata ttcaggccca acagaagaaa gaactgaata
     7081 gttttctcga gtcccagaaa agagagtata aacttcgaaa agagcagctt aaagaggagc
     7141 taaatgaaaa ccagagtacc cccaaaaaag aaaaacagga gtggctttca aagcagaagg
     7201 agaatataca gcatttccaa gcagaagaag aagctaacct tcttcgacgt caaagacaat
     7261 acctagagct ggaatgccgt cgcttcaaga gaagaatgtt acttgggcgt cataacttag
     7321 agcaggacct tgtcagggag gagttaaaca aaagacagac tcagaaggac ttagagcatg
     7381 ccatgctact ccgacagcat gaatctatgc aagaactgga gttccgccac ctcaacacaa
     7441 ttcagaagat gcgctgtgag ttgatcagat tacagcatca aactgagctc actaaccagc
     7501 tggaatataa taagcgaaga gaacgagaac taagacgaaa gcatgtcatg gaagttcgac
     7561 aacagcctaa gagtttgaag tctaaagaac tccaaataaa aaagcagttt caggatacct
     7621 gcaaaatcca aaccagacag tacaaagcat taagaaatca cctgctggag actacaccaa
     7681 agagtgagca caaagctgtt ctgaaacggc tcaaggagga acagacccgg aaattagcta
     7741 tcttggctga gcagtatgat cacagcatta atgaaatgct ctccacacaa gccctgcgtt
     7801 tggatgaagc acaggaagca gagtgccagg ttttgaagat gcagctgcag caggaactgg
     7861 agctgttgaa tgcgtatcag agcaaaatca agatgcaagc tgaggcacaa catgatcgag
     7921 agcttcgcga gcttgaacag agggtctccc tccggagggc actcttagaa caaaagattg
     7981 aagaagagat gttggctttg cagaatgagc gcacagaacg aatacgaagc ctgttggaac
     8041 gtcaagccag agagattgaa gcttttgact ctgaaagcat gagactaggt tttagtaata
     8101 tggtcctttc taatctctcc cctgaggcat tcagccacag ctacccggga gcttctggtt
     8161 ggtcacacaa ccctactggg ggtccaggac ctcactgggg tcatcccatg ggtggcccac
     8221 cacaagcttg gggccatcca atgcaaggtg gaccccagcc atggggtcac ccttcagggc
     8281 caatgcaagg ggtacctcga ggtagcagta tgggagtccg caatagcccc caggctctga
     8341 ggcggacagc ttctggggga cggacagagc agggcatgag cagaagcacg agtgtcactt
     8401 cacaaatatc caatgggtca cacatgtctt atacatcaaa cccagctttc ttgtacaaag
     8461 tgggcggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcatcccgc
     8521 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
     8581 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
     8641 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     8701 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     8761 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     8821 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     8881 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     8941 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     9001 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc
     9061 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga
     9121 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     9181 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     9241 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagtaagcc aactttatta
     9301 tacatagttg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     9361 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9421 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9481 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9541 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9601 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9661 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9721 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     9781 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9841 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9901 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc
     9961 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
    10021 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
    10081 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
    10141 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
    10201 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
    10261 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
    10321 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
    10381 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
    10441 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
    10501 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
    10561 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
    10621 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
    10681 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
    10741 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
    10801 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
    10861 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
    10921 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
    10981 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
    11041 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
    11101 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
    11161 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
    11221 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
    11281 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    11341 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    11401 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
    11461 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
    11521 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11581 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
    11641 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    11701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11761 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
    11821 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
    11881 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11941 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    12001 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    12061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    12121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    12181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    12241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    12301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    12361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    12421 atttaacgcg aattttaaca aaatattaac gcttacaatt t
//
