LOCUS       sequence-374319-        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tdTomato protein and mouse Synaptophysin protein fused 
            with EGFP in Cre expressing mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Gesuita L, Argunsah AO, Karayannis T
  TITLE     Sparse postnatal labeling and quantification of superficial cortical
            cell synapses in the mouse neocortex.
  JOURNAL   STAR Protoc. 2022 Nov 11;3(4):101837. doi: 
            10.1016/j.xpro.2022.101837. eCollection 2022 Dec 16.
  PUBMED    36386881
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-11-11"; volume: "3(4):101837. doi"; pages: " 
            10.1016/j.xpro.2022.101837. eCollection 2022 Dec 16"
COMMENT     Sequence Label: pCALNL-tdTomato-2A-mSyp::EGFP
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        13..392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..670
                     /label=chicken beta-actin promoter
     intron          671..1687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1611..1633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1695..1714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1762..1795)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1845..2639
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1899..1918)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2509..2528
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2819..2953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2967..3000)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3061..3070
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3200..3218)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3468..3487
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3662..3685
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4501..4554
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5503..5505,5506..5508,5509..6222)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5503..5505,5506..5508,5509..6219)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5548..5569)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5809..5828)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6156..6177
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6327..6346)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6392..6447
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6446..6465)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6808..6824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6808..6824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6821..6843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6832..6848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6856..6862,6863..6880,6881..6886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6901..6922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      7007..7141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7161..7295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7363..7380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7534..8122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7614..7633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8293..9084,9085..9153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9154..9258)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
       61 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      121 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      181 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      241 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      301 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      361 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
      421 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
      481 tttgtgcagc gatgggggcg gggggggggg gggnsgcgcg ccrggsgggg sggggsgggg
      541 sgrggggsgg ggsggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      601 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      661 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      721 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      781 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
      841 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
      961 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1021 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1081 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1141 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1201 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1261 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 gattaattcg agcgaacgcg tataacttcg tatagcatac attatacgaa gttatctcga
     1801 gtcggatttg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     1861 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     1921 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     1981 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     2041 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     2101 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     2161 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     2221 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     2281 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     2341 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     2401 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     2461 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     2521 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     2581 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     2641 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     2701 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     2761 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccatcgataa
     2821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2941 tcatgtctgg atcaatccga acgcgtataa cttcgtatag catacattat acgaagttat
     3001 ctcgagtcgc tcggtacgat ttaaattgaa ttctgcagtc gacggtaccg cgggcccggg
     3061 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     3121 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     3181 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     3241 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     3301 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     3361 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     3421 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     3481 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     3541 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     3601 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     3661 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     3721 gagggccgcc accacctgtt cctggggcat ggtaccggca gcaccggcag cggcagctcc
     3781 ggcaccgcct cctccgagga caacaacatg gccgtgatta aggagtttat gaggtttaaa
     3841 gtcaggatgg agggcagcat gaacgggcac gagtttgaga ttgaaggaga gggggaaggg
     3901 aggccctatg agggcaccca gacagcaaag ctgaaagtga caaagggcgg ccctctgcca
     3961 ttcgcatggg acatcctgtc tcctcagttt atgtatggaa gtaaagccta cgtgaaacac
     4021 cctgctgaca ttccagatta caagaaactg agcttcccag aggggtttaa gtgggaaaga
     4081 gtgatgaact tcgaagacgg gggcctggtg actgtcaccc aggacagctc tctgcaggac
     4141 ggcactctga tctataaggt gaaaatgcgc ggcaccaatt ttccccctga tggcccagtc
     4201 atgcagaaga agacaatggg ctgggaggct tcaacagaaa ggctgtaccc cagagacggc
     4261 gtgctgaagg gagagatcca ccaggcactg aagctgaaag atggagggca ttatctggtc
     4321 gaattcaaaa ccatctacat ggccaagaaa cccgtgcagc tgcctggcta ctattacgtc
     4381 gacacaaagc tggatattac tagccataac gaagactaca ccattgtgga gcagtatgag
     4441 cggagcgagg ggaggcacca tctgtttctg tacggcatgg acgagctgta caagactagt
     4501 gagggcagag gaagtcttct gacatgcgga gacgtggaag agaatcccgg ccctgcccca
     4561 ggctcatcta gaatggacgt ggtgaatcag ctggtggctg ggggtcagtt ccgggtggtc
     4621 aaggagcccc ttggcttcgt gaaggtgctg cagtgggtct ttgccatctt cgcctttgct
     4681 acgtgcggca gctacaccgg agagcttcgg ctgagcgtgg agtgtgccaa caagacggag
     4741 agtgccctca acatcgaagt cgaatttgag tacccattca ggctgcacca agtgtacttt
     4801 gatgcaccct cctgcgttaa agggggcact accaagatct tcctagttgg tgactactcc
     4861 tcctcggctg aattctttgt caccgtggct gtgtttgcct tcctctactc catgggggcc
     4921 ctggccacct acatcttcct gcagaacaag taccgagaga acaacaaagg gccaatgatg
     4981 gacttcctgg ccacagcagt gttcgctttc atgtggctag ttagctcatc cgcctgggcc
     5041 aaaggcctgt ccgatgtgaa gatggccact gacccagaga acattatcaa ggagatgcct
     5101 atgtgccgcc agacaggaaa cacatgcaag gaactgaggg accctgtgac ttcaggactc
     5161 aacacctcgg tggtgtttgg cttcctgaac ctggtgctct gggttggcaa cctatggttc
     5221 gtgttcaagg agacaggctg ggccgcccca ttcatgcgcg cacctccagg cgccccagaa
     5281 aagcaaccag ctcctggcga tgcctacggc gatgcgggct atgggcaggg ccccggaggc
     5341 tatgggcccc aggactccta cgggcctcag ggtggttatc aacccgatta cgggcagcca
     5401 gccagcggcg gtggcggtgg ctacgggcct cagggcgact atgggcagca aggctacggc
     5461 caacagggtg cgcccacctc cttctccaat cagatgtcgc gaatggtgag caagggcgag
     5521 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5581 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5641 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5701 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5761 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5821 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5881 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5941 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     6001 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6061 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6121 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6181 gccgccggga tcactctcgg catggacgag ctgtacaagt aatctagagg cgcgccaaat
     6241 agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     6301 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     6361 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     6421 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     6481 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     6541 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     6601 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     6661 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     6721 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     6781 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6961 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7081 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7141 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     7201 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7261 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     7321 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7441 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7501 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8101 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8161 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8221 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9241 acaaataggg gttccgcgca catttccccg aaaagt
//
