LOCUS       sequence-374366-        9387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single construct out of the pool of plasmid for lineage recording 
            experiment using DNA Ticker Tape..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, 
            Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J
  TITLE     A time-resolved, multi-symbol molecular recorder via sequential 
            genome editing.
  JOURNAL   Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 
            10.1038/s41586-022-04922-8.
  PUBMED    35794474
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 
            10.1038/s41586-022-04922-8."
COMMENT     Sequence Label: Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC
FEATURES             Location/Qualifiers
     source          1..9387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      487..1075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     976..995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1229..1246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1363..1384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1399..1404,1405..1422,1423..1429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1437..1453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1442..1464
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1461..1477
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1461..1477
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1496..1516
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1498..1516
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1544..1770
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1771..1951
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1998..2123
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2616..2849
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3034..3078
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3259..3376
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3515..4693
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3746..4684
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4641..4661
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4706..5302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4706..4725)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5202..5222
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5312..5368
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5369..5371,5372..5374,5375..6088)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5369..5371,5372..5374,5375..6085)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5414..5435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5675..5694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6022..6043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6326..6914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6379..6399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6797..6808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7070..7318
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7070..7090
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7241..7260
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7339..7414
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             7496..7676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7754..7875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7791..7810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7845..7864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7915..8050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8070..8089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8071..8089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8099..8116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8099..8115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8108..8130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8257..8712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8344..8363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8554..8575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8738..8842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8843..8911,8912..9387,1..316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9061..9080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
       61 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
      121 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
      181 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      241 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
      301 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      361 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      421 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      481 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      541 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      601 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      661 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      721 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      781 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      841 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      901 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      961 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1021 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1081 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1141 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1201 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1261 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1321 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     1381 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     1441 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     1501 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     1561 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     1621 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     1681 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     1741 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     1801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1921 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1981 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     2041 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     2101 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     2161 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     2221 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     2281 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     2341 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     2401 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     2461 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     2521 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     2581 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     2641 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     2701 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     2761 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     2821 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     2881 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     2941 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     3001 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     3061 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     3121 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     3181 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaatct tgagacaaat
     3241 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3301 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3361 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     3421 cgagggggcc cgggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     3481 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     3541 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc
     3601 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     3661 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     3721 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     3781 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     3841 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     3901 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     3961 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     4021 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     4081 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     4141 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     4201 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     4261 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     4321 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     4381 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     4441 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     4501 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     4561 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     4621 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     4681 tcaggtgtcg tgacgtacgg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     4741 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4801 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4861 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4921 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4981 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5041 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc
     5101 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5161 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5221 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5281 catgacccgc aagcccggtg ccggaagcgg agctactaac ttcagcctgc tgaagcaggc
     5341 tggcgacgtg gaggagaacc ctggacctat ggtgagcaag ggcgaggagc tgttcaccgg
     5401 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5461 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5521 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5581 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5641 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5701 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5761 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5821 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5881 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5941 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6001 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6061 tctcggcatg gacgagctgt acaagtgaag tcgactttcc aagctccata ggtctgattt
     6121 ggtaccatag cagatgatcc atggtcaatt tagaagagga tgatggtgag cacgtgatgg
     6181 tgagcacgtg atggtgagca cgtgatggtg agcacgtgat ggtgagcacg tgatggtgag
     6241 ccaaggaaag gaagccctgc ttcctccaga gggcgtcgca ggacagctgc tttaaggccg
     6301 gtcctagcaa tcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6721 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6841 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6901 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     6961 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7021 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
     7081 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
     7141 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
     7201 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
     7261 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
     7321 atgatggtga gcacgtgagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     7381 ttatcaactt gaaaaagtgg caccgaatcg gtgctcacca tcatccatgc gtgctcacca
     7441 tcttttttta agcttggcgt aactagatct tgagacactg ctttttgctt gtactgggtc
     7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     7681 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7741 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7861 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7921 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7981 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8041 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     8101 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8161 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8221 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     8281 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8341 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8401 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8461 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8521 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8581 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8641 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8701 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8761 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8821 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8881 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8941 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9001 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9061 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9121 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9181 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9241 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9301 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9361 cgacgagcgt gacaccacga tgcctgt
//
