LOCUS       sequence-374464-       12093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express wild-type APP from Ubiquitin C promoter, co-expressing 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12093)
  TITLE     Hart Lab APP and APOE Plasmids 2022
REFERENCE   2  (bases 1 to 12093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-APPwt-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..12093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(1570..1581)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             2061..2081
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             3328..3381
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3394..3414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3415..4125
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3560..3578)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3828..3847
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4022..4045
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4164..4752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4217..4237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4635..4646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4755..4771)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4756..4772)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5277..5457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5483..5500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5489..5713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5759..6187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5846..5865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6056..6077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6196..6216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6201..6530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6381..6516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6578..6625
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6644..7018
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7148..7269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7185..7204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7239..7258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7318..7334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7318..7334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7331..7353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7342..7358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7366..7372,7373..7390,7391..7396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7411..7432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7549..7566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7720..8308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7800..7819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8479..9270,9271..9339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9102..9121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9340..9444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9519..9538)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9710..10089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10091..10289
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10244..10264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10307..10487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10534..10659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11152..11385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11570..11614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11912..12029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactggcca ccatgctgcc cggtttggca
     1261 ctgctcctgc tggccgcctg gacggctcgg gcgctggagg tacccactga tggtaatgct
     1321 ggcctgctgg ctgaacccca gattgccatg ttctgtggca gactgaacat gcacatgaat
     1381 gtccagaatg ggaagtggga ttcagatcca tcagggacca aaacctgcat tgataccaag
     1441 gaaggcatcc tgcagtattg ccaagaagtc taccctgaac tgcagatcac caatgtggta
     1501 gaagccaacc aaccagtgac catccagaac tggtgcaagc ggggccgcaa gcagtgcaag
     1561 acccatcccc actttgtgat tccctaccgc tgcttagttg gtgagtttgt aagtgatgcc
     1621 cttctcgttc ctgacaagtg caaattctta caccaggaga ggatggatgt ttgcgaaact
     1681 catcttcact ggcacaccgt cgccaaagag acatgcagtg agaagagtac caacttgcat
     1741 gactacggca tgttgctgcc ctgcggaatt gacaagttcc gaggggtaga gtttgtgtgt
     1801 tgcccactgg ctgaagaaag tgacaatgtg gattctgctg atgcggagga ggatgactcg
     1861 gatgtctggt ggggcggagc agacacagac tatgcagatg ggagtgaaga caaagtagta
     1921 gaagtagcag aggaggaaga agtggctgag gtggaagaag aagaagccga tgatgacgag
     1981 gacgatgagg atggtgatga ggtagaggaa gaggctgagg aaccctacga agaagccaca
     2041 gagagaacca ccagcattgc caccaccacc accaccacca cagagtctgt ggaagaggtg
     2101 gttcgagttc ctacaacagc agccagtacc cctgatgccg ttgacaagta tctcgagaca
     2161 cctggggatg agaatgaaca tgcccatttc cagaaagcca aagagaggct tgaggccaag
     2221 caccgagaga gaatgtccca ggtcatgaga gaatgggaag aggcagaacg tcaagcaaag
     2281 aacttgccta aagctgataa gaaggcagtt atccagcatt tccaggagaa agtggaatct
     2341 ttggaacagg aagcagccaa cgagagacag cagctggtgg agacacacat ggccagagtg
     2401 gaagccatgc tcaatgaccg ccgccgcctg gccctggaga actacatcac cgctctgcag
     2461 gctgttcctc ctcggcctcg tcacgtgttc aatatgctaa agaagtatgt ccgcgcagaa
     2521 cagaaggaca gacagcacac cctaaagcat ttcgagcatg tgcgcatggt ggatcccaag
     2581 aaagccgctc agatccggtc ccaggttatg acacacctcc gtgtgattta tgagcgcatg
     2641 aatcagtctc tctccctgct ctacaacgtg cctgcagtgg ccgaggagat tcaggatgaa
     2701 gttgatgagc tgcttcagaa agagcaaaac tattcagatg acgtcttggc caacatgatt
     2761 agtgaaccaa ggatcagtta cggaaacgat gctctcatgc catctttgac cgaaacgaaa
     2821 accaccgtgg agctccttcc cgtgaatgga gagttcagcc tggacgatct ccagccgtgg
     2881 cattcttttg gggctgactc tgtgccagcc aacacagaaa acgaagttga gcctgttgat
     2941 gcccgccctg ctgccgaccg aggactgacc actcgaccag gttctgggtt gacaaatatc
     3001 aagacggagg agatctctga agtgaagatg gatgcagaat tccgacatga ctcaggatat
     3061 gaagttcatc atcaaaaatt ggtgttcttt gcagaagatg tgggttcaaa caaaggtgca
     3121 atcattggac tcatggtggg cggtgttgtc atagcgacag tgatcgtcat caccttggtg
     3181 atgctgaaga agaaacagta cacatccatt catcatggtg tggtggaggt tgacgccgct
     3241 gtcaccccag aggagcgcca cctgtccaag atgcagcaga acggctacga aaatccaacc
     3301 tacaagttct ttgagcagat gcagaacgag ggcaggggaa gtcttctaac atgcggggac
     3361 gtggaggaaa atcccggccc tctagagatg gctccaaaga agaagcgtaa ggtaatggtg
     3421 agcaagggcg aggaggacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     3481 atggagggct ccgtgaacgg ccgcgagttc gagatcgagg gcgagggcga gggccgcccc
     3541 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     3601 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     3661 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     3721 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     3781 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     3841 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     3901 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgccgaggtc
     3961 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     4021 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc
     4081 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaattaa gctagtctag
     4141 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4741 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     4801 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     4861 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     4921 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4981 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5041 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5101 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     5161 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     5221 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     5281 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5341 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5401 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5461 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5521 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5581 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5641 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5701 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5761 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5821 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5881 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5941 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6001 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6061 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6121 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6181 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6361 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6421 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6481 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6541 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     6601 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     6661 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     6721 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     6781 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     6841 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     6901 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     6961 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7021 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7081 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7141 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7201 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7261 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9481 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     9541 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     9601 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     9661 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     9721 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     9781 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9841 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9901 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9961 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    10021 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10081 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    10141 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    10201 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    10261 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
    10321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    10501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    10561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    10921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    10981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11761 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11821 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
    11881 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    11941 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    12001 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    12061 tttggttaat taacccgtgt cggctccaga tct
//
