LOCUS       sequence-375054-        8968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8968)
  TITLE     Cantley Lab plasmids
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGEP-sh-Hs-PHGDH-1967
FEATURES             Location/Qualifiers
     source          1..8968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             163..343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(344..570)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(598..618)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(598..616)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(637..653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(637..653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(650..672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    661..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(685..691,692..709,710..715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    730..751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(868..885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1039..1627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1119..1138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1798..2589,2590..2658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2421..2440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2659..2763)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2789..3244)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2926..2947)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3138..3157
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3371..3393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3385..3402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3386..3402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3412..3431
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3412..3430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3451..3586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3626..3747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3637..3656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3691..3710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3819..4052
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4259..4847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4365..4376
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4774..4794
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4928..5527)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5011..5031)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5508..5527
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5548..6047)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5596..5615)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5989..6012
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     ncRNA           complement(6226..6240)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(join(6434..7147,7148..7150,7151..7153))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6479..6500)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6828..6847
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7087..7108
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(7176..7582)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7620..7737
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8004..8048)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8233..8466
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    join(8959..8968,1..116)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
       61 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
      121 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
      181 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
      241 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
      301 cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg tatcgagcta
      361 ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca
      421 gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc
      481 atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc
      541 aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct tttgttccct
      601 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      661 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      721 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      841 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      901 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      961 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1021 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1081 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1141 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1201 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1261 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1321 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1381 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1441 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1501 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     1981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2761 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     2821 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2881 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2941 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3001 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3061 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3241 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3301 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     3361 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     3421 tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct
     3481 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     3541 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     3601 gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca aaccacaact
     3661 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3721 accattataa gctgcaataa acaagttcct ctcactctct gatattcatt tctttgcaag
     3781 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     3841 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     3901 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     3961 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc
     4021 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct
     4081 aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctgaggtg tgactggaaa
     4141 acccacctcc tcctcctctt gtgcttctag ccaggcacaa tcagcattgg tagctgctgt
     4201 attgctactt gtgattgctc catgtttttc taggtctcga ctgcagaatt aattccaggc
     4261 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     4321 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     4381 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga
     4441 aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     4501 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     4561 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     4621 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     4681 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     4741 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     4801 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtt ccagacgcgt
     4861 ttcgaagtcg acatgcatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg
     4921 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     4981 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     5041 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     5101 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     5161 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     5221 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     5281 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     5341 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     5401 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     5461 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     5521 cggtcatggt aagcttgggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag
     5581 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
     5641 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
     5701 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
     5761 cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat
     5821 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
     5881 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga
     5941 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
     6001 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt agaattacgc
     6061 gtcaattgaa aaaagtgatt taatttatac cattttaatt cagctttgta aaaatgtatc
     6121 aaagagatag caaggtattc agttttagta aacaagataa ttgctcgaat tctagcccct
     6181 tgaagtccga ggcagtaggc agtacagcaa taaccgtcta atatacatct gtggcttcac
     6241 tatattagac ggttattgct gtatcgctca ctgtcaacag caatatacct tctcgagcct
     6301 tctgttgggt taagaagtcg aagtaatccc agcaagtgtt tccaagatgt gcaggcaacg
     6361 attctgtaaa gtactgaagc ctcattcaaa caattaccct gttatcccta gtcgaggcgg
     6421 ccgccgtacg tcattacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     6481 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     6541 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     6601 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     6661 tcgccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     6721 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     6781 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     6841 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     6901 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     6961 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     7021 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc agacacgctg aacttgtggc
     7081 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     7141 ccttgctcac catggtggat ccaccggtag atccaccggc cgactcagtc tgtcggagga
     7201 ctggcgcgcc gagtgagggg ttgtgagctc ttttatagag ctcgggaagc agaagcgcgc
     7261 gaacagaagc gagaagcagg ctgattggtt aattcaaata aggcgcaggg tcatttcagg
     7321 tccttggggg agcctggaaa catctgatgg gtcttaagaa actgctgagg gttgggccat
     7381 atctggggac catctgttct tggcctcggg ccggggccga aactgcggtg accatctgtt
     7441 cttggccccg ggccggggcc gaaactgctc accgcagata tcctgtttgg cccaacgtta
     7501 gctattttca tgtacccgcc cttgatctga acttctctat tcttggtttg gtatttttcc
     7561 atgccttgca aaatggcgtt actgcagcta gctcgatcga ggctagtctc gtgatcgata
     7621 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
     7681 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaagtt
     7741 aaccgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
     7801 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
     7861 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     7921 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     7981 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     8041 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     8101 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     8161 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     8221 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     8281 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     8341 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     8401 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     8461 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     8521 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     8581 tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt
     8641 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     8701 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     8761 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     8821 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     8881 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     8941 tctcccccgc ttaatactga cgctctcg
//
