LOCUS       sequence-375174-        3600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses K2P4.1 with C-terminal StrepTagII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, 
            Zhang T, Russell D, Baker ES, Yan X, Laganowsky A
  TITLE     Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine 
            Interactions.
  JOURNAL   J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 
            10.1021/jacs.2c00612. Epub 2022 Apr 14.
  PUBMED    35421309
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c00612"; journalName: "J Am Chem Soc"; date: 
            "2022-04-27- 27"; volume: "144"; issue: "16"; pages: "7048-7053"
COMMENT     Sequence Label: pACE-K2P4.1-StrepTagII
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(94..99,100..116,117..122))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(193..226)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        303..394
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     319..336
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1299..1322
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1349..1483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1386..1405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1440..1459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1604..1769
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1959..2547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2448..2467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2682..2905
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2972..3505)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
       61 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
      121 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
      181 agcgaagtcg ccataacttc gtatagcata cattatacga agttatctgt aactataacg
      241 gtcctaaggt agcgagttta aacactagta tcgattcgcg acctactccg gaatattaat
      301 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
      361 tcgtaacagt tttgtaataa aaaaacctat aaataaccgc caccatgact accgctcctc
      421 aagagccacc agcaagaccc cttcaagcag gctcaggcgc aggtcccgct cctggccgtg
      481 ccatgaggtc aaccactttg ttggctctgc ttgctttagt gctgctttac ctagtcagtg
      541 gtgccctggt gtttagggcc ttggaacaac cccacgagca acaagctcag cgtgagctgg
      601 gtgaggtcag agaaaaattt ttaagagcac acccatgtgt atccgaccaa gaacttggac
      661 tgctaatcaa agaggtggcc gacgcccttg gcggcggcgc cgaccctgaa acccaatcca
      721 cctctcagtc aagtcacagt gcctgggatc tgggaagtgc cttctttttc agtggtacaa
      781 ttatcacgac aataggctac ggaaacgtgg ccttgaggac ggatgccggt agactgtttt
      841 gcatattcta cgcattagta ggaatccccc tgtttggtat cttgctagct ggtgtgggtg
      901 atcgtctggg atcatcactt agacatggta taggccatat agaggccatc ttcctgaaat
      961 ggcatgtacc accagaattg gtcagggttt tatcagctat gttattttta cttatcggat
     1021 gcctattgtt tgtactgacc ccaacattcg ttttctgcta catggaagac tggtccaaac
     1081 ttgaagctat ttattttgtg atagtgacct taactacggt cggctttggc gattacgtcg
     1141 caggcgcaga tccacgtcaa gattctccag catatcaacc actagtgtgg ttctggatcc
     1201 tactgggatt agcctacttc gcttctgtct tgactactat aggcaactgg ttgagagtcg
     1261 tttcccgtag gacccgtgca gaaatgggta ccgcttcctg gtcacatcca cagtttgaga
     1321 aataataatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1381 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1441 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     1501 tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1561 agcaataact atcataaccc ctagggtata cccatctaat tggaaccaga taagtgaaat
     1621 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     1681 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     1741 ccagttccca actattttgt ccgcccacaa ccggttgact tgggtcaact gtcagaccaa
     1801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2521 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2581 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttgacttggg
     2641 tcgctcttcc tgtggatgcg cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt
     2701 cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag
     2761 ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc
     2821 taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg
     2881 ccaagggcat gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg
     2941 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     3001 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     3061 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     3121 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     3181 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     3241 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     3301 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacatcg ccgaactcac
     3361 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     3421 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     3481 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     3541 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
//
