LOCUS       sequence-37518-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     ALK5 Plasmids
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5F-ALK5-HA(ecto)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(33..56)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(360..386)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(514..533)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(546..564)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(547..564)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(774..798)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aacaagttgg gccatggcgg ccaagcttat cacttgtcgt cgtcgtcctt gtagtccatg
       61 tcgacttcca caggaccaag gccaggtgat gactttacag tagttggaag ttctatttta
      121 ttgcaatggt cctgattgca gcaatatgtt gtagtcacag acccagtttt tgaagagggt
      181 gcacatacaa acggcctatc tcgaggaatt aagtcaattt cagctataca catgctgttg
      241 tgtataactt tgtctgtggt ctctgtgaca gagacaaagc agagcccatc tgtcacacaa
      301 gtaaaattgt cttttgtaca gaggtggcag aaacactgta acgccgtcgc ccccgggagg
      361 gcgtagtctg ggacgtcgta tgggtacagc gccgccgccg ccgccgccgc cgccgccagc
      421 acgaggagga gcagccgggg acgcggagca gcgaccgccg cctccatgaa ttcaatcgat
      481 agaaccgagg tgcagttgga cctgggagtg gacacctgtg gagagaaagg caaagtggat
      541 gttattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      601 tagccgcgtt ctaacgaagc caagggggtg ggcctataga ctctataggc ggtacttacg
      661 tcactcttgg cacggggaat ccgcgttcca atgcaccgtt cccggccgcg gaggctggat
      721 cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
      781 cggttcacta aacgagctct gcttatatag acctcc
//
