LOCUS       sequence-37522-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: CMV-Brainbow-1.0 L
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    191..312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(228..247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     282..301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(395..428)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      442..451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(493..514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(754..773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
       61 gtaaagtgtg cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
      121 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      181 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      301 caatgtatct taaggcgtaa attgtaagcg ttaatgctcc gagggccgcc accacctgtt
      361 cctgtaggaa ttggccgcga ctctaggatc taggataact tcgtatagga tactttatac
      421 gaagttattg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      541 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
      661 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      841 aaggaggacg gcaacatc
//
