LOCUS       sequence-37531-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Stowers RS
  TITLE     An efficient method for recombineering GAL4 and QF drivers.
  JOURNAL   Fly (Austin). 2011 Oct 1;5(4).
  PUBMED    21857163
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin). 2011 Oct 1;5(4)."
COMMENT     Sequence Label: pGAL4Kan
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             259..699
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 ctaagggaca aagctggcca ctgtttaaac ttggcgcgcc tttgcgatcg cagacctagg
       61 aaagctagct atacgcgtta taccggtatc ctgcaggtag tatgcaagag atcttatagg
      121 cctcgagtta acgttcgagg tcgactctag ataaagcttt agagctcttc ttaagaagga
      181 tcccgtaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag cccaagcttg
      241 aagcaagcct cctgaaagat gaagctactg tcttctatcg aacaagcatg cgatatttgc
      301 cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac
      361 aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg
      421 acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga
      481 gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca
      541 ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg
      601 gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa
      661 gagagtagta acaaaggtca aagacagttg actgtatcga ttgactcggc agctcatcat
      721 gataactcca caattccgtt ggattttatg cccagggatg ctcttcatgg atttgattgg
      781 tctgaagagg atgacatgtc ggatggcttg cccttcctga aaacggaccc caacaataat
      841 gggttctttg gcgacggttc tctcttatgt attcttcgat ctattggctt taaaccggaa
      901 aattacacga actctaacg
//
