LOCUS       sequence-375475-        4084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY for Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pDL191
FEATURES             Location/Qualifiers
     source          1..4084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(842..941)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1053..1072)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1131..1150)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1724..2257)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2446..2451,2452..2468,2469..2474))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3131..3719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3620..3639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3854..3881)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3973..4059)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggcttcg aaggagatag aaccatgggt cgacctagag gtgatgaaaa gtactggaat
      181 gaagtaacga gttttgaaga gaacgggaag cagaagtgga agtgcaagca ttgtgaaaaa
      241 acgtatggtg gaggttattc aagaattaaa ctgcatttag ataaagaaca aaaaggacaa
      301 ggcattacta aatgccccgt ttattgtagc aatgaagaag ttctcaacat cattgcttcc
      361 acttcaatcc atccacaaga aactgtaaat acactgaatc cattacaagt ggttgaagat
      421 gttgttgctg gcgaaatgat tgtccctgtt ggtgctccaa ttaaccatca aacaaataat
      481 gaatcaacaa gtttattcga aggttgggca ccagagcatg ctattgtcgg aagcaacaat
      541 tcgaatggta ttttccaaag taagattagt gtgataaatg agttggtttc cgatctatca
      601 agagaggaag atgacatcaa ggaacaatta gaatggctag aatctcgcgg caagaagcgc
      661 aagggtgatg ttgatgactg gatggatgaa ctacgagacc tgaagaaaca tgttgctgat
      721 gtggattggt tagggttcac taacttttat gcatcggatt tggttgataa attgcgaaag
      781 cataagattg ataagcctct ggtgttgtca aatgaatttg ttggtgaaca atttgagtta
      841 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
      901 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg
      961 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     1021 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1081 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1141 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     1201 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1261 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1321 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     1381 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1441 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1501 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1561 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     1621 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1681 ggggaaatta ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     1741 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     1801 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     1861 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     1921 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     1981 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     2041 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     2101 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     2161 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     2221 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     2281 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     2341 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     2401 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     2461 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     2521 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     2581 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     2641 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     2701 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     2761 ttgttcgccc aggactctag ctatagttct agtggttggc tactaatagg ttgtattgat
     2821 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2881 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2941 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3001 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc caggaagagt
     3841 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3901 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     3961 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4021 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4081 tcgc
//
