LOCUS       sequence-375614-       13747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ADAR1, p150 isoform in plant-expressable format..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, 
            Sattely ES, Gao XJ
  TITLE     Modular, programmable RNA sensing using ADAR editing in living 
            cells.
  JOURNAL   Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x.
  PUBMED    36198772
REFERENCE   2  (bases 1 to 13747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 
            10.1038/s41587-022-01493-x."
COMMENT     Sequence Label: EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)
FEATURES             Location/Qualifiers
     source          1..13747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        270..315
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             842..907
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4604..4621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4874..5126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5258..5603
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    6230..6406
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6972..7151
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7004..7187
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7208..8002
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7262..7281)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7872..7891
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8388..8640
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8731..8755
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      9123..9754
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(9844..10992)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11291..12085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(12675..12692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12926..12945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    13377..13401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13540..13559
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13684..13706
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13698..13715
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13699..13715
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgagaa gatagtggaa
       61 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      121 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      181 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
      241 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
      301 tttcatttgg agaggtatta aaatcttaat aggttttgat aaaagcgaac gtggggaaac
      361 ccgaaccaaa ccttcttcta aactctctct catctctctt aaagcaaact tctctcttgt
      421 ctttcttgcg tgagcgatct tcaacgttgt cagatcgtgc ttcggcacca gtacaacgtt
      481 ttctttcact gaagcgaaat caaagatctc tttgtggaca cgtagtgcgg cgccattaaa
      541 taacgtgtac ttgtcctatt cttgtcggtg tggtcttggg aaaagaaagc ttgctggagg
      601 ctgctgttca gccccataca ttacttgtta cgattctgct gactttcggc gggtgcaata
      661 tctctacttc tgcttgacga ggtattgttg cctgtacttc tttcttcttc ttcttgctga
      721 ttggttctat aagaaatcta gtattttctt tgaaacagag ttttcccgtg gttttcgaac
      781 ttggagaaag attgttaagc ttctgtatat tctgcccaaa ttcgcgaccg gtatgcgggc
      841 tgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
      901 tgacaagggg cgcgccctag taaatccgcg gcaggggtat tccctcagcg gatactacac
      961 ccatccattt caaggctatg agcacagaca gctcagatac cagcagcctg ggccaggatc
     1021 ttcccccagt agtttcctgc ttaagcaaat agaatttctc aaggggcagc tcccagaagc
     1081 accggtgatt ggaaagcaga caccgtcact gccaccttcc ctcccaggac tccggccaag
     1141 gtttccagta ctacttgcct ccagtaccag aggcaggcaa gtggacatca ggggtgtccc
     1201 caggggcgtg catctcggaa gtcaggggct ccagagaggg ttccagcatc cttcaccacg
     1261 tggcaggagt ctgccacaga gaggtgttga ttgcctttcc tcacatttcc aggaactgag
     1321 tatctaccaa gatcaggaac aaaggatctt aaagttcctg gaagagcttg gggaagggaa
     1381 ggccaccaca gcacatgatc tgtctgggaa acttgggact ccgaagaaag aaatcaatcg
     1441 agttttatac tccctggcaa agaagggcaa gctacagaaa gaggcaggaa cacccccttt
     1501 gtggaaaatc gcggtctcca ctcaggcttg gaaccagcac agcggagtgg taagaccaga
     1561 cggtcatagc caaggagccc caaactcaga cccgagtttg gaaccggaag acagaaactc
     1621 cacatctgtc tcagaagatc ttcttgagcc ttttattgca gtctcagctc aggcttggaa
     1681 ccagcacagc ggagtggtaa gaccagacag tcatagccaa ggatccccaa actcagaccc
     1741 aggtttggaa cctgaagaca gcaactccac atctgccttg gaagatcctc ttgagttttt
     1801 agacatggcc gagatcaagg agaaaatctg cgactatctc ttcaatgtgt ctgactcctc
     1861 tgccctgaat ttggctaaaa atattggcct taccaaggcc cgagatataa atgctgtgct
     1921 aattgacatg gaaaggcagg gggatgtcta tagacaaggg acaacccctc ccatatggca
     1981 tttgacagac aagaagcgag agaggatgca aatcaagaga aatacgaaca gtgttcctga
     2041 aaccgctcca gctgcaatcc ctgagaccag aagaaacgca gagttcctca cctgtaatat
     2101 acccacatca aatgcctcaa ataacatggt aaccacagaa aaagtggaga atgggcagga
     2161 acctgtcata aagttagaaa acaggcaaga ggccagacca gaaccagcaa gactgaaacc
     2221 acctgttcat tacaatggcc cctcaaaagc agggtatgtt gactttgaaa atggccagtg
     2281 ggccacagat gacatcccag atgacttgaa tagtatccgc gcagcaccag gtgagtttcg
     2341 agccatcatg gagatgccct ccttctacag tcatggcttg ccacggtgtt caccctacaa
     2401 gaaactgaca gagtgccagc tgaagaaccc catcagcggg ctgttagaat atgcccagtt
     2461 cgctagtcaa acctgtgagt tcaacatgat agagcagagt ggaccacccc atgaacctcg
     2521 atttaaattc caggttgtca tcaatggccg agagtttccc ccagctgaag ctggaagcaa
     2581 gaaagtggcc aagcaggatg cagctatgaa agccatgaca attctgctag aggaagccaa
     2641 agccaaggac agtggaaaat cagaagaatc atcccactat tccacagaga aagaatcaga
     2701 gaagactgca gagtcccaga cccccacccc ttcagccaca tccttctttt ctgggaagag
     2761 ccccgtcacc acactgcttg agtgtatgca caaattgggg aactcctgcg aattccgtct
     2821 cctgtccaaa gaaggccctg cccatgaacc caagttccaa tactgtgttg cagtgggagc
     2881 ccaaactttc cccagtgtga gtgctcccag caagaaagtg gcaaagcaga tggccgcaga
     2941 ggaagccatg aaggccctgc atggggaggc gaccaactcc atggcttctg ataaccagcc
     3001 tgaaggtatg atctcagagt cacttgataa cttggaatcc atgatgccca acaaggtcag
     3061 gaagattggc gagctcgtga gatacctgaa caccaaccct gtgggtggcc ttttggagta
     3121 cgcccgctcc catggctttg ctgctgaatt caagttggtc gaccagtccg gacctcctca
     3181 cgagcccaag ttcgtttacc aagcaaaagt tgggggtcgc tggttcccag ccgtctgcgc
     3241 acacagcaag aagcaaggca agcaggaagc agcagatgcg gctctccgtg tcttgattgg
     3301 ggagaacgag aaggcagaac gcatgggttt cacagaggta accccagtga caggggccag
     3361 tctcagaaga actatgctcc tcctctcaag gtccccagaa gcacagccaa agacactccc
     3421 tctcactggc agcaccttcc atgaccagat agccatgctg agccaccggt gcttcaacac
     3481 tctgactaac agcttccagc cctccttgct cggccgcaag attctggccg ccatcattat
     3541 gaaaaaagac tctgaggaca tgggtgtcgt cgtcagcttg ggaacaggga atcgctgtgt
     3601 aaaaggagat tctctcagcc taaaaggaga aactgtcaat gactgccatg cagaaataat
     3661 ctcccggaga ggcttcatca ggtttctcta cagtgagtta atgaaataca actcccagac
     3721 tgcgaaggat agtatatttg aacctgctaa gggaggagaa aagctccaaa taaaaaagac
     3781 tgtgtcattc catctgtata tcagcactgc tccgtgtgga gatggcgccc tctttgacaa
     3841 gtcctgcagc gaccgtgcta tggaaagcac agaatcccgc cactaccctg tcttcgagaa
     3901 tcccaaacaa ggaaagctcc gcaccaaggt ggagaacgga gaaggcacaa tccctgtgga
     3961 atccagtgac attgtgccta cgtgggatgg cattcggctc ggggagagac tccgtaccat
     4021 gtcctgtagt gacaaaatcc tacgctggaa cgtgctgggc ctgcaagggg cactgttgac
     4081 ccacttcctg cagcccattt atctcaaatc tgtcacattg ggttaccttt tcagccaagg
     4141 gcatctgacc cgtgctattt gctgtcgtgt gacaagagat gggagtgcat ttgaggatgg
     4201 actacgacat ccctttattg tcaaccaccc caaggttggc agagtcagca tatatgattc
     4261 caaaaggcaa tccgggaaga ctaaggagac aagcgtcaac tggtgtctgg ctgatggcta
     4321 tgacctggag atcctggacg gtaccagagg cactgtggat gggccacgga atgaattgtc
     4381 ccgggtctcc aaaaagaaca tttttcttct atttaagaag ctctgctcct tccgttaccg
     4441 cagggatcta ctgagactct cctatggtga ggccaagaaa gctgcccgtg actacgagac
     4501 ggccaagaac tacttcaaaa aaggcctgaa ggatatgggc tatgggaact ggattagcaa
     4561 accccaggag gaaaagaact tttatctctg cccagtacta gtccaccatc accatcacca
     4621 ttagctcgag gcctttaact ctggtttcat taaattttct ttagtttgaa tttactgtta
     4681 ttcggtgtgc atttctatgt ttggtgagcg gttttctgtg ctcagagtgt gtttatttta
     4741 tgtaatttaa tttctttgtg agctcctgtt tagcaggtcg tcccttcagc aaggacacaa
     4801 aaagatttta attttattaa aaaaaaaaaa aaaaaaaaga ccgggaattc gatatcaagc
     4861 ttatcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     4921 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     4981 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5041 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     5101 cgcggtgtca tctatgttac tagatctcta gagtctcaag cttggcgcgc cagcttggcg
     5161 taatcatggt catagctgtt gcgattaaga attcgagctc ggtacccccc tactccaaaa
     5221 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     5281 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
     5341 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
     5401 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     5461 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
     5521 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     5581 gttcatttca tttggagagg acagcccaag cttcgactct agaggatccc cttaaatcga
     5641 tatggaacga gctatacaag gaaacgacgc tagggaacaa gctaacagtg aacgttggga
     5701 tggaggatca ggaggtacca cttctccctt caaacttcct gacgaaagtc cgagttggac
     5761 tgagtggcgg ctacataacg atgagacgaa ttcgaatcaa gataatcccc ttggtttcaa
     5821 ggaaagctgg ggtttcggga aagttgtatt taagagatat ctcagatacg acaggacgga
     5881 agcttcactg cacagagtcc ttggatcttg gacgggagat tcggttaact atgcagcatc
     5941 tcgatttttc ggtttcgacc agatcggatg tacctatagt attcggtttc gaggagttag
     6001 tatcaccgtt tctggagggt ctcgaactct tcagcatctc tgtgagatgg caattcggtc
     6061 taagcaagaa ctgctacagc ttgccccaat cgaagtggaa agtaatgtat caagaggatg
     6121 ccctgaaggt actcaaacct tcgaaaaaga aagcgagtaa gtcgagggcg agctcgaatt
     6181 cggtacgctg aaatcaccag tctctctcta caaatctatc tctctctatt ttctccataa
     6241 ataatgtgtg agtagtttcc cgataaggga aattagggtt cttatagggt ttcgctcatg
     6301 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
     6361 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatctcct aaagtcccta
     6421 tagatctttg tcgtgaatat aaaccagaca cgagacgact aaacctggag cccagacgcc
     6481 gttcgaagct agaagtaccg cttaggcagg aggccgttag ggaaaagatg ctaaggcagg
     6541 gttggttacg ttgactcccc cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa
     6601 ggaggaagac aaggaaggat aaggttgcag gccctgtgca aggtaagaag atggaaattt
     6661 gatagaggta cgctactata cttatactat acgctaaggg aatgcttgta tttataccct
     6721 atacccccta ataacccctt atcaatttaa gaaataatcc gcataagccc ccgcttaaaa
     6781 attggtatca gagccatgaa taggtctatg accaaaactc aagaggataa aacctcacca
     6841 aaatacgaaa gagttcttaa ctctaaagat aaaagatggc gcgtggccgg ccatgagcgg
     6901 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt
     6961 tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa
     7021 tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact
     7081 atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg
     7141 gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg gatctggatc
     7201 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7261 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7321 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7381 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7441 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7501 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7561 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7621 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7681 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     7741 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7801 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7861 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7921 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7981 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     8041 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8101 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8161 ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat cattacgaca
     8221 gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgc ggcgtccaca
     8281 tcaacggcgt cggcggcgac tgcccaggca agaccgagat gcaccgcgat atcttgctgc
     8341 gttcggatat tttcgtggag ttcccgccac agacccggat gatccccgat cgttcaaaca
     8401 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     8461 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     8521 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     8581 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     8641 gggggccggc cctcactggt gaaaagaaaa accaccccag tacattaaaa acgtccgcaa
     8701 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     8761 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     8821 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     8881 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     8941 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaaatatca
     9001 tctccctcgc agagatccga attatcagcc ttcttattca tttctcgctt aaccgtgaca
     9061 gagtagacag gctgtctcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg
     9121 ttagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
     9181 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
     9241 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
     9301 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
     9361 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
     9421 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
     9481 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
     9541 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
     9601 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg
     9661 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
     9721 cccagcggcg agggcaacca gcccggtgag cgtcggaaag gcgctcggtc ttgccttgct
     9781 cgtcggtgat gtacactagt cgctggctgc tgaaccccca gccggaactg accccacaag
     9841 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
     9901 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
     9961 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    10021 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    10081 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    10141 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    10201 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    10261 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    10321 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    10381 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    10441 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    10501 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    10561 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    10621 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    10681 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    10741 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    10801 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    10861 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    10921 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    10981 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    11041 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    11101 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    11161 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
    11221 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
    11281 catctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    11341 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    11401 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    11461 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    11521 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    11581 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    11641 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    11701 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    11761 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    11821 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    11881 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    11941 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    12001 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    12061 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    12121 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    12181 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    12241 agtatcgacg gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt
    12301 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
    12361 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
    12421 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
    12481 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc
    12541 ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag
    12601 tcggtaacta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    12661 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12721 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12781 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    12841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    12901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    13021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    13081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    13141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcaggt
    13201 aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgggccccc
    13261 ggcgccagat ctggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    13321 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    13381 acccgccaat atatcctgtc aaacactgat agtttgtgaa ccatcaccca aatcaagttt
    13441 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    13501 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    13561 gggcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    13621 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    13681 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gttaattaag aattcgagct
    13741 ccaccgc
//
