LOCUS       sequence-37571-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pENTR L1-20XUAS-L4
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    122..216
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    250..344
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    378..472
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    506..600
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        625..863
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     810..831
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(873..893)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcatatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggctct caagcttgca tgcctgcagg
      121 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      181 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcc ctagggcatg
      241 cctgcaggtc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
      301 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagcgct
      361 agcgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      421 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact
      481 ctagcactag tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
      541 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      601 cggagactct agcgacgtcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
      661 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
      721 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
      781 aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta
      841 ttgaatacaa gaagagaact ctgaatagca cccaactttt ctatacaaag ttggcattat
      901 aagaaagcat tgcttatcaa tttgttg
//
