LOCUS       sequence-375789-        9616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin 
            dehydroxylase promoter-lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ8.1-Pcadh-LacZ
FEATURES             Location/Qualifiers
     source          1..9616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      30..101
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(282..870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(362..381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1041..1832,1833..1901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1664..1683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1902..2006)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2074..2092
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2249..2268
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2557..5631
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2577..2594)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2577..2593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2586..2608)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5649..5676
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5799..6593)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ccgccaagct tgctggggct tttctttttc aaataaaacg aaaggctcag tcgaaagact
       61 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagg agctcatgcc
      121 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
      181 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
      241 gcagccgcac gcggcgcatc tcgggccgcg ttgctggcgt ttttccatag gctccgcccc
      301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      721 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1321 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     1381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     1681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2101 cttcaaggcg aaaaaacccc gccgaagcgg ggttttttgc gaaatataat gaccctcttg
     2161 ataacccaag agggcatttt ttaggaacgg ttatttgatt attaatccgg cggcaactga
     2221 aacgattcgg atcctgtatt actattctga attcgcggcc gcttctagat gcggaccccc
     2281 ttttactcac atccccatgg cgataaggct cgttcgagca ttgtcgtcag tatagcaatt
     2341 cgccgatacc gcggcggcgg gcggatggcg aaataccact ctcggtggta ggtctttcat
     2401 gagctcgata gtagggtttc tgacctggtt gaacagagtt cgcatcagcg gtggggtgct
     2461 caactcgcgc tcgatctgca tcatggttcg cacggacgct tggtccggcc cgctcgttgg
     2521 agtgcgggtg aaaagggaac cggcagagga ggagctatga ccatgattac ggattcactg
     2581 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2641 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2701 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     2761 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     2821 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
     2881 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     2941 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     3001 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     3061 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     3121 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     3181 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     3241 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     3301 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     3361 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     3421 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     3481 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     3541 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     3601 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     3661 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     3721 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
     3781 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     3841 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     3901 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
     3961 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
     4021 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     4081 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     4141 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     4201 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
     4261 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
     4321 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
     4381 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
     4441 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     4501 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     4561 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     4621 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     4681 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
     4741 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
     4801 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
     4861 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     4921 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
     4981 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     5041 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
     5101 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     5161 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
     5221 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     5281 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     5341 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     5401 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     5461 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     5521 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
     5581 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata aactagtcga
     5641 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata
     5701 aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa aaaaaggatc
     5761 aattttgaac tctctcccaa agttgatccc ttaacgatct aaaacaattc atccagtaaa
     5821 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     5881 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     5941 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     6001 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     6061 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     6121 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     6181 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     6241 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     6301 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     6361 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     6421 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     6481 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     6541 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     6601 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     6661 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     6721 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
     6781 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
     6841 agtttttatg ctttgcccat tctatagata tattgataag aagactggaa tgcccgctcc
     6901 gccgggatta ttcgaaagcg catcgtccaa gcgagaccgt ctgcattcgg aaatctcggc
     6961 cggttcgcgc cgaggaagcc catctggctg catcccaaat cgacacacaa cgcacggagg
     7021 ccccggcgat cgccggggcc tccgaatcat gcgcatcgtg gaagcgaagc tccgcaaaaa
     7081 accccgcttc agcggggttt tttcgcgtcg cacctccttg tcgtgtatta caggtactac
     7141 tataacacat catctagccc gcccgcgata cgtcctctgc ggtgcagcgt ggtcgcggtt
     7201 ccacgcatcc gccgcccgct gcgcatcgcg cagcacgtca ttaaggctct gttgccttgg
     7261 ctgttccctg cgcatcagat attcgagcgg gcgcgatatt cgacttctaa gctcttccac
     7321 tttgattcgc attcccgcaa ctcctgctct gatgcggctc gcgagtccat cgcacttaac
     7381 aacgatgtta tcgaggattg cgccgcgctc agcttttccc gcagcttccc agccccgaac
     7441 ctcggtagcg atgggttgaa gctctccaat gtctcgcgca actccgtccg ctcgttgccg
     7501 aagacactcc aatcggtctt gctcatcttc gatatcgagc ttcacgtcct cgagctggct
     7561 gtcaagctcg gcaaggtcac cctccttgtc ggcaatctcg gtaacaagct ctgccttacg
     7621 cgtttcggca tcattcagta aagcggcggc ttcttcccta gccgccgcga cgattcgcgc
     7681 cgcttcgtcc ttcgcaggtt caaccacggc cttatcgacc gcacagcgta ccttgtcgat
     7741 atccacggac ttgtccgtgt atacacgctg cgcccgcgtc tcctcgtcaa gctcgattgc
     7801 agggcggtag cccatgcgcc cctctaggta atccccaagc tccttgtgca tgctccggta
     7861 gaagctgcgg gggcacatct gcttgtagtt gaaccgtcca tctaggatgg gcgtgaacgg
     7921 cacgtgcatg tggtcgcggg cgggcgtgcc atccttcaac acctcgtcct tgtggacgta
     7981 cccgcccatc atgttctcga tgccgaactt cttcgacagg taccagtacg tgagacggaa
     8041 gaacctgtcc tcatcgccct tgcgcacatt gtcgggaagc gtcacaacaa cgtcagccat
     8101 aacgaccgca tccttgcgcg tggcacgctt ccctgcggcc ttctgggcct cgttcacgcg
     8161 ctcgatgcgg ctctccaccg tgccccagtt cggctcgcca tcgcgcggct ccacgcgccc
     8221 cacaacgagc ttgccgtcct tgttcgtgta gtggccgacc accaagtcgt tcttgatgcg
     8281 ctcggggtcg atgttgtcgc gctccaggct gctgcggtcg cggcggtagt gggcaagcat
     8341 gtgcccgacc gatggagcct tgtatttcgc gatgtgagcc atggaaacct ccgaaccgta
     8401 cagacttaat cgactaaagt ctagcacgtt agaccttagc tacgctaagg acgtggcagg
     8461 gccagccctg cacccgctcg ggcgttcccc ccgagacccc ccagcccttg cccgttcgcg
     8521 gcttcgctca cggccctcgg gccatcggcg cgagcgccga acggagggac ttcgcccctc
     8581 cgaaccaccc ctcaatctac ctgcggttcg gcacacggca cacggcacta ttaggggggc
     8641 cgtcgtagtg gcccccctgt ggtcatttgt catttgtcat ttgtcacatt atttttcgga
     8701 cacattattt ttcggacgta gttagcgaaa aataatgagc cgcccgattc tgcaggtctc
     8761 atccagtcga gacacgggca aggaaagggt cgatggagca gagcgtccat cgacccttaa
     8821 tcaagtctgg tctattcggt tggccgtcgc gtcaattccg caatccgtga acgagtcggc
     8881 ttagatctgc gcgagcgcag accacgccgc cccgtagtca cgcattgctg cattgacccg
     8941 actactcttt ggcacctgac tgttccacgc acttgcgctt acgttcggcg ttcagatagt
     9001 tcccgcgaca atagcgctcg agaatcgagt tgtaccccac gatgatttcg cggtactgct
     9061 cgggaaactc cctcagcgcc gcagccgtga aatcgttgat ggtctcgaac ctctcgcaca
     9121 tgatgaagcc gaccaactcg aagagcatgt cctgcttgtc cttagtcgag agctgttcga
     9181 gctgaccaat gtcgaagttg tttccctcga tgcgtgcttc gaccggatac agctccttgc
     9241 ccttcttgcg acaggaagcc gtgtcgtgaa tcagatagtc atacgcatgc ctgatgttgt
     9301 tgattggctc ggctgacgaa cagcacttct tgccgtccgc gctcaatcgg ttcaagacgt
     9361 tgatgattgc cttgcgtgtc gtgttgccgc cccagacaac aatgacgtgc gcatgcgtct
     9421 tgcgctgctt gctcttctgg tcatggtcta tgtcgtgaac cgcataagcg aaaggcactt
     9481 gaagaatgtc gtctatctcg tcctgccagt catcgcgcat gttctctggg tagcagatgc
     9541 cctcccagag cttcaccttc gtctcgccag cgtttgccgc catgctataa tctccttgtc
     9601 tcgaatgccc ctgctg
//
