LOCUS       sequence-375793-       13701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-BirA 
            3xFLAG-NUP37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK BirA* 3xFLAG-NUP37 zeo
FEATURES             Location/Qualifiers
     source          1..13701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3001..3351,3352..3354,3355..3963)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3973..4038
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5052..5076)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5126..6337
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6174..6193
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6396..7013,7014..7019,7020..7055,7056..7058,7059..7094,
                     7095..7097,7098..7133,7134..7142)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7180..7753
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7347..7364)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7574..7593
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        7775..7822
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7841..8215
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8389..8977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8442..8462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8860..8871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8980..8996)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8981..8997)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9506..9686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9712..9729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9718..9942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9988..10416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10075..10094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10285..10306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10425..10445)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10430..10759
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10610..10745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10672..10691
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10807..10854
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10873..11247
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11377..11498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11414..11433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11468..11487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11547..11563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11547..11563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11560..11582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11571..11587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11595..11601,11602..11619,11620..11625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11640..11661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11778..11795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11949..12537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12029..12048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12708..13499,13500..13568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13331..13350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13569..13673)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactagt gcgcactaga
     3001 atgaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
     3061 tctggcgagc agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc
     3121 cagacactga gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg
     3181 cctgagccta tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc
     3241 gtggccgtgc tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag
     3301 ctgaagtccg gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga
     3361 cggaagtggt tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag
     3421 cagggacctg ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg
     3481 ctgagaaagc tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag
     3541 gaccgcaagc tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag
     3601 atcgtgatcg gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac
     3661 cagggctgga tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc
     3721 atgctgatca gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct
     3781 tacctgagca gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc
     3841 ggcgacaagg aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg
     3901 gagcaggacg gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag
     3961 aagactagta tggactacaa agaccatgac ggtgattata aagatcatga catcgattac
     4021 aaggatgacg atgacaagat gaagcaagat gcctcaagaa atgctgccta cactgtggat
     4081 tgtgaagatt atgtgcatgt ggtagaattt aatccctttg agaatgggga ttcaggaaac
     4141 ctaattgcat atggtggcaa taattatgtg gtcattggca cgtgtacgtt tcaggaagaa
     4201 gaagcagacg ttgaaggcat tcagtataaa acacttcgaa catttcacca tggagtcagg
     4261 gttgatggca tagcttggag cccagagact agacttgatt cattgcctcc agtaatcaaa
     4321 ttttgtactt cagctgctga tatgaaaatt agattattta cttcagatct tcaggataaa
     4381 aatgaatata aggttttaga gggccatacc gatttcatta atggtttggt gtttgatccc
     4441 aaagaaggcc aagaaattgc aagtgtgagt gacgatcaca cctgcaggat ttggaacttg
     4501 gaaggagtgc aaacagctca ttttgttctt cattctcctg gcatgagtgt gtgctggcat
     4561 cctgaggaga cttttaagct aatggttgca gagaagaatg gaacaatccg gttttatgat
     4621 cttttggccc aacaggctat tttatctctt gaatcagaac aagtgccatt aatgtcagca
     4681 cactggtgct taaaaaacac cttcaaagtt ggagccgttg caggaaatga ttggttaatt
     4741 tgggatatta ctcggtccag ttatcctcaa aataagagac ctgttcacat ggatcgagcc
     4801 tgcttattca ggtggtccac aattagtgaa aatctgtttg caaccactgg ttatcctggc
     4861 aaaatggcaa gccagtttca aattcatcat ttaggacacc ctcagcccat cctcatgggt
     4921 tctgtagccg ttggatctgg actgtcctgg catcgaactc tccctctgtg tgtaattgga
     4981 ggagaccaca agctgttttg ggtgactgaa gtataacaat tggaagacta atgcgttttc
     5041 gagatatcta gacccagctt tcttgtacaa agtggttgat aacatctcga gccataaaga
     5101 tggttaatta acccacccaa gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     5161 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     5221 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     5281 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     5341 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     5401 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     5461 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     5521 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     5581 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     5641 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     5701 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     5761 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     5821 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     5881 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     5941 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     6001 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     6061 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     6121 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     6181 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     6241 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     6301 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat
     6361 ctccaccccg taccggtcct gcagtcgaat tcaccatgtc tagactggac aagagcaaag
     6421 tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga
     6481 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     6541 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
     6601 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     6661 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     6721 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     6781 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     6841 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     6901 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     6961 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     7021 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     7081 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt
     7141 aactaagtaa ggatccgcgg ccgcactaga ggaattccgc ccctctcccc cccccccccc
     7201 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     7261 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     7321 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     7381 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     7441 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     7501 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     7561 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     7621 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     7681 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7741 aacacgatga taagcttacc ggtacgcgtg atgtgttgac aattaatcat cggcatagta
     7801 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7861 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7921 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7981 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8041 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8101 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8161 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacat
     8221 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     8281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     8341 caattgttgt tgtttagtcc ctcccaattc gatatcaagc ttatcgataa tcaacctctg
     8401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8761 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8941 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     9001 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     9061 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     9121 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     9181 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     9241 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     9301 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     9361 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     9421 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     9481 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     9541 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9601 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9661 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     9721 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9781 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9841 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9901 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9961 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
    10021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10141 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10201 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10261 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10321 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10381 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
    10441 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10501 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10561 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10621 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10681 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
    10741 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
    10801 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
    10861 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    10921 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    10981 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    11041 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    11101 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    11161 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    11221 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
    11281 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    11341 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    11401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    11461 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    11521 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11581 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11641 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11701 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11761 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    12061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12121 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12361 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    12421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    12601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    13021 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    13081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    13141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13321 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13681 ccccgaaaag tgccacctga c
//
