LOCUS       sequence-375872-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell line generation via recombination-mediated cassette exchange 
            (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Villasenor R, Pfaendler R, Ambrosi C, Butz S, Giuliani S, Bryan E, 
            Sheahan TW, Gable AL, Schmolka N, Manzo M, Wirz J, Feller C, von 
            Mering C, Aebersold R, Voigt P, Baubec T
  TITLE     ChromID identifies the protein interactome at chromatin marks.
  JOURNAL   Nat Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2.
  PUBMED    32123383
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Mar 2. pii: 10.1038/s41587-020-0434-2. doi: 
            10.1038/s41587-020-0434-2."
COMMENT     Sequence Label: pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             357..377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             720..740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcattattg tttttcctcc tttgccgcca ccatggacta caaagacgat gacgacaagg
       61 atgaagagtc tggttctgtt tctggtggtg atgagctgtc agccataggc gagcaggtgt
      121 ttgcggtgga gagcatccgg aagaagcgcg tgcggaaggg caaagttgaa tatctggtga
      181 agtggaaagg atggcccccc aagtatagca cctgggagcc agaggagcac atcttggacc
      241 ctcgccttgt catggcctac gaggagaagg aggagagaga ccgagcctcg gggtatagga
      301 agagaggtcc gaaacccatc gttggcggag gctctggagg tggaggctcc gcacccccca
      361 aaaagaaacg caaggttgga taccgggatc caggcggggg gtctagtgca aaggcacctg
      421 tccgaagtgt cgtcacagaa actgtgtcta cttatgtcat cagagacgaa tggggaaatc
      481 agatttggat ctgtcccggc tgcaataagc cagatgacgg atcacctatg ataggatgtg
      541 acgactgcga tgactggtat cactggcctt gcgttgggat catggccgcc ccccccgaag
      601 agatgcaatg gttttgcccc aaatgcgcca ataaaataaa aaaggacaag aaacataaga
      661 aacgcaagca tcgcgctcac atcggtggcg gaggctctgg aggtggaggc tccgcacccc
      721 ccaaaaagaa acgcaaggtt ggataccggg atccacacgt gggtaggagc ggttcgttcg
      781 gatcccatct tttttcggaa ttcggatccg gtggaggcgg gtctggaggc gggggtagtg
      841 gcgggggtgg aagcgggggt ggaggcgggt cgggtggcgg aggtagcgga ggcggtggaa
      901 gtggaggcgg tggaagtggt g
//
