LOCUS       sequence-37588-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Osborn MJ, Defeo AP, Blazar B, Tolar J
  TITLE     Synthetic Zinc Finger Nuclease Design and Rapid Assembly.
  JOURNAL   Hum Gene Ther. 2011 Jun 13.
  PUBMED    21663559
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2011 Jun 13."
COMMENT     Sequence Label: pMJO-6 (minus T7 RNA polymerase binding site)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             130..195
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             202..222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgactctctg gctactgaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg cagcaccatg ggacctaaga aaaagaggaa ggtggcggcc
      121 gccaccatgg attataagga tcacgatggc gactacaagg accacgatat tgactacaaa
      181 gacgatgacg ataaaatggc tcctaagaaa aagcgcaaag tcggtatcca tggcgttccc
      241 tctagataac gcaggatccc agctggtgaa gtccgagctg gaagaaaaaa agagcgagct
      301 gcgccacaag ctcaagtacg tgccccacga gtacatcgag ctgatcgaga tcgcccgcaa
      361 cagcacccaa gaccgcatcc tggagatgaa agtgatggag ttcttcatga aggtgtacgg
      421 ctaccgcggc aagcacctgg gcggctcccg caagcccgat ggcgccatct acaccgtggg
      481 ctcccccatc gactatggcg tcattgtcga caccaaggcc tactccggcg gctacaactt
      541 acccatcggt caggccgacg agatgcaacg ctacgtgaag gagaaccaga cccgcaataa
      601 gcacattaat cccaacgagt ggtggaaggt gtacccctcc tccgtgaccg agttcaaatt
      661 cctgttcgtg tccggccact tcaagggcaa ttataaggcc caactgaccc gcctgaacca
      721 caagaccaac tgcaacggcg ccgtgctgtc cgtggaggaa ctgctgatcg gcggcgagat
      781 gatcaaggct ggtaccctga ccctggaaga ngtgcgccgc aagttcaaca atggtgaaat
      841 caatttcagg tccggcggcg
//
