LOCUS       sequence-375972-       11819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible
            promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. 
            lenta A2 strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11819)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 11819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70DA9-Pct5-DadhABC(A2)
FEATURES             Location/Qualifiers
     source          1..11819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3019..3813
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(3936..3963)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    8568..8595
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             8933..9544
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     misc_feature    9547..9566
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(9723..9741)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9809..9913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9914..9982,9983..10774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10132..10151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10945..11533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11434..11453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(11714..11785)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggggcattcg agacaaggag attatagcat ggcggcaaac gctggcgaga cgaaggtgaa
       61 gctctgggag ggcatctgct acccagagaa catgcgcgat gactggcagg acgagataga
      121 cgacattctt caagtgcctt tcgcttatgc ggttcacgac atagaccatg accagaagag
      181 caagcagcgc aagacgcatg cgcacgtcat tgttgtctgg ggcggcaaca cgacacgcaa
      241 ggcaatcatc aacgtcttga accgattgag cgcggacggc aagaagtgct gttcgtcagc
      301 cgagccaatc aacaacatca ggcatgcgta tgactatctg attcacgaca cggcttcctg
      361 tcgcaagaag ggcaaggagc tgtatccggt cgaagcacgc atcgagggaa acaacttcga
      421 cattggtcag ctcgaacagc tctcgactaa ggacaagcag gacatgctct tcgagttggt
      481 cggcttcatc atgtgcgaga ggttcgagac catcaacgat ttcacggctg cggcgctgag
      541 ggagtttccc gagcagtacc gcgaaatcat cgtggggtac aactcgattc tcgagcgcta
      601 ttgtcgcggg aactatctga acgccgaacg taagcgcaag tgcgtggaac agtcaggtgc
      661 caaagagtag tcgggtcaat gcagcaatgc gtgactacgg ggcggcgtgg tctgcgctcg
      721 cgcagatcta agccgactcg ttcacggatt gcggaattga cgcgacggcc aaccgaatag
      781 accagacttg attaagggtc gatggacgct ctgctccatc gaccctttcc ttgcccgtgt
      841 ctcgactgga tgagacctgc agaatcgggc ggctcattat ttttcgctaa ctacgtccga
      901 aaaataatgt gtccgaaaaa taatgtgaca aatgacaaat gacaaatgac cacagggggg
      961 ccactacgac ggccccccta atagtgccgt gtgccgtgtg ccgaaccgca ggtagattga
     1021 ggggtggttc ggaggggcga agtccctccg ttcggcgctc gcgccgatgg cccgagggcc
     1081 gtgagcgaag ccgcgaacgg gcaagggctg gggggtctcg gggggaacgc ccgagcgggt
     1141 gcagggctgg ccctgccacg tccttagcgt agctaaggtc taacgtgcta gactttagtc
     1201 gattaagtct gtacggttcg gaggtttcca tggctcacat cgcgaaatac aaggctccat
     1261 cggtcgggca catgcttgcc cactaccgcc gcgaccgcag cagcctggag cgcgacaaca
     1321 tcgaccccga gcgcatcaag aacgacttgg tggtcggcca ctacacgaac aaggacggca
     1381 agctcgttgt ggggcgcgtg gagccgcgcg atggcgagcc gaactggggc acggtggaga
     1441 gccgcatcga gcgcgtgaac gaggcccaga aggccgcagg gaagcgtgcc acgcgcaagg
     1501 atgcggtcgt tatggctgac gttgttgtga cgcttcccga caatgtgcgc aagggcgatg
     1561 aggacaggtt cttccgtctc acgtactggt acctgtcgaa gaagttcggc atcgagaaca
     1621 tgatgggcgg gtacgtccac aaggacgagg tgttgaagga tggcacgccc gcccgcgacc
     1681 acatgcacgt gccgttcacg cccatcctag atggacggtt caactacaag cagatgtgcc
     1741 cccgcagctt ctaccggagc atgcacaagg agcttgggga ttacctagag gggcgcatgg
     1801 gctaccgccc tgcaatcgag cttgacgagg agacgcgggc gcagcgtgta tacacggaca
     1861 agtccgtgga tatcgacaag gtacgctgtg cggtcgataa ggccgtggtt gaacctgcga
     1921 aggacgaagc ggcgcgaatc gtcgcggcgg ctagggaaga agccgccgct ttactgaatg
     1981 atgccgaaac gcgtaaggca gagcttgtta ccgagattgc cgacaaggag ggtgaccttg
     2041 ccgagcttga cagccagctc gaggacgtga agctcgatat cgaagatgag caagaccgat
     2101 tggagtgtct tcggcaacga gcggacggag ttgcgcgaga cattggagag cttcaaccca
     2161 tcgctaccga ggttcggggc tgggaagctg cgggaaaagc tgagcgcggc gcaatcctcg
     2221 ataacatcgt tgttaagtgc gatggactcg cgagccgcat cagagcagga gttgcgggaa
     2281 tgcgaatcaa agtggaagag cttagaagtc gaatatcgcg cccgctcgaa tatctgatgc
     2341 gcagggaaca gccaaggcaa cagagcctta atgacgtgct gcgcgatgcg cagcgggcgg
     2401 cggatgcgtg gaaccgcgac cacgctgcac cgcagaggac gtatcgcggg cgggctagat
     2461 gatgtgttat agtagtacct gtaatacacg acaaggaggt gcgacgcgaa aaaaccccgc
     2521 tgaagcgggg ttttttgcgg agcttcgctt ccacgatgcg catgattcgg aggccccggc
     2581 gatcgccggg gcctccgtgc gttgtgtgtc gatttgggat gcagccagat gggcttcctc
     2641 ggcgcgaacc ggccgagatt tccgaatgca gacggtctcg cttggacgat gcgctttcga
     2701 ataatcccgg cggagcgggc attccagtct tcttatcaat atatctatag aatgggcaaa
     2761 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     2821 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     2881 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     2941 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     3001 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     3061 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     3121 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     3181 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     3241 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     3301 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     3361 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     3421 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     3481 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     3541 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     3601 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     3661 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     3721 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     3781 aaatattata ttttactgga tgaattgttt tagatcgtta agggatcaac tttgggagag
     3841 agttcaaaat tgatcctttt tttataacag gaattaaata ataaaaaagc cggattaata
     3901 atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac ccgaaggtga
     3961 gccagtgtga gtcgactagt ttagtacatc aggaccactg ggtagcccag ctggtagatc
     4021 agcacgcgaa acatcacgga ggtggccagc gcgccgatgg cggcgacgac catgagcgtc
     4081 ttggaggcgc ccaccttctt cttcgccacg aaggcgcagg ccgcagccac gatcacggag
     4141 acgatggagc cccagaacat gggcgctccc gcgccggaca ccatgacggt catcaggctg
     4201 tcgacgtgcg tgggggccgt ggtcatcgat gtcgggtcgg cgtagtagcc gaattgcgcg
     4261 atcttcgcca tggcgcaggc gatgacgaac accacgtcgg ccacgatctg cacgagggcc
     4321 gcgatgaacg tcagctggat gcccgtcgcc tcgacggcct cgtccttctt caggatggag
     4381 atcagccaga cggccgcggc gcccaggagg cacgcgttgc ccagatagaa cgccacgagc
     4441 gccaggcccc acgcgggacg ggccggcatg aggtaggagt gcgcggtggc gaccatcatg
     4501 agcacggcaa ccgcgagggt cgcccaggcc agcgccttgg gaacgggctt gccgcctcga
     4561 aggaccacga accatgcgac gatgacgatg gccagcgcca cgcatccgat gagctcctgg
     4621 gtgatgcccg acgtgatgtg cccgaagccg ttgaagatgc gctcccagtg ctcgaggtgc
     4681 aggaaggctc cgatgccgcc taccaccagc gagacggcgg acgtgatgag cgcggtcatc
     4741 tgcaggttcc tgccctggcc cttgagcgcc aggatggaca tgccgcccag cgtgccgcag
     4801 gtcaagcata cgaacagcgt gaagatgacg agaggccact gcgtttccat tactcatcac
     4861 ctccttccca gtgatggtcg cgtaggatgt aggagtacga cggcttgttg cccacgtcgg
     4921 gcagcttgtg cacgtcggcg tcggtgaacg gctccaggaa ctccaccatc ttgcggcgct
     4981 tgcccagcgg gtcctcggag ccctcgaacg actcgtagcc ggcctcggtg tcgccgatcc
     5041 agcgggcgtt gccgccgcac tggctgacgc actggggcag ctcgccctgc gacagcttct
     5101 gctcgcacag cgtgcacttc tcgacgacct tctcctcctc gttgaggtag cggacgccgt
     5161 aggggcaggc catgacgcag aactggcagc cgatgcactt ctccttgtcg atctggatgg
     5221 agccgtcctc ggccacgtgc gacgcctcgg tggggcacac cttcacgcac tcggggttct
     5281 cgcagtgctg gcagctgatg ggcaggaagt acatgtacac gtcggggtac tgccctgatc
     5341 cgcccgggat ggggttcggc cccacgcgca ccactttgtt ccagtagctg ccgatgggca
     5401 cgttgttgac cgccttgcag gcgaccgagc acgccaggca gcccacgcac cggttgaggt
     5461 cggtgatgat agccttcgtc atggtttact ccctcccttc gtaggtaggc agccattcct
     5521 tcagacgggg gtcgtcggag gtatggatga tgggcgtgcc ggtggagtcg cacggcacgg
     5581 ggttgttgaa cggcgagttc tcgggcgtgg ccttgtagat cttcacctgg taggcgcgca
     5641 ggttggacgt gccggagtgc gggtcctggg cgtagtggtc gatcagctgg ttcacctggg
     5701 agaactgcca gccgtggccg gcgtccttca cctcggggta ccaccacgtg tgctcgaggt
     5761 tgatgacgtc ttccttcacg ccgtagtaca ggtcagccac ttcgcggatc ttgccccact
     5821 cggtttcgat ccacacccag tcgccctgct cgatgccgta ctcggcggcc gtcttcgggt
     5881 tgatctccac gcgcggcacg ggccacagct cgcggcacca gggaagctgg cgatgctcgg
     5941 agtggaagta caccgggatg cgacggccgg tggtggcggt cagcgggtac tccttgatgc
     6001 ggtcgccgtc cttcgggccg tgaggcggct cggtgtaggt gggaagcctc cactcgggat
     6061 ggtcgggatg gtgcgattcc atgacggtgg accagatctc ctgcttcatc gtcggggtga
     6121 accagcccgg cttccagtcg ccgatgccct tgccggccgt gtagtcgagg cggccccaca
     6181 cgcggtcgcg tgcgcgcatc gcgccggtct ggtagcggcg gtacgtgccc cagttgcgcg
     6241 gctcgatgtc cttcgcctgc caccagccgt gctcctggaa ggccttgacg tactcgtccc
     6301 acgtcttgta cttgggcgtg acctcggcca tcggaacgcc gtggcgctcg aacgtgggct
     6361 tgccgttctc gacgtgccac gtggtgtagg tcagctcgtc gtcggtgagc agcttgatgg
     6421 agtcgtccag ctgccagttg atgtcgggcc actcgttgcc cggctcgtcg gtccacttcc
     6481 agttcatgcg gcgggcgagg tccatgacga tctcgggatc gtacttggct tccgcgggcg
     6541 gctgcacgca cttgaccgtg gcgcccatgg cgccggcgga accttggctg gcgcgcggcg
     6601 agttgagctc gatccaatgg gccaccggca ggatgatgtc ggcggtgtcg ttctgcggcg
     6661 tgtgccacag gttgaggtcc acgtagaagt caagcgagca cagggcctcc caggcgcgcg
     6721 tggtgttcgt ctggctcatg aagttgccgg actcgttcca gagggcgcgc accgggtagg
     6781 ggtcgcccgt gatgacggcg tcccacagcg aatggctgtc gcaccagtac tgccaccagc
     6841 ccagaagcgg gaacttgtcg atgccgatct gcttgcggtt gacttcctcc ggcggggtgg
     6901 tggcgccggg ggcccaggcg ctgaagccct ggaggtcgcc gtcgatgggg acgatggtcg
     6961 ggccgcggtt gccgcccggg gtgtccatgt tgccggtgat gcccacgagg ttgtcgaagg
     7021 cgcggcagtt ctggatggcg ctgcagaagt gctcgaccgc cagcatgtac tggatgccgc
     7081 cgttgccgta gccggtggac gggtcgatgc gcgtgccgta ggccgtggcc gccgcctcga
     7141 tctcggaggc ggggatgccg gtgatctcgg ccgccacctc gggcttgtac tcggcggcgc
     7201 gggccttgta gtgctcccac accggcttga ccttcacggt cttgccgtcc ttcagcgtga
     7261 tctcgaactc gccttccagc gcggggtcga tggcggggtc gaacggcatg ggatcgggca
     7321 gccagccctg cgtctggccc ggcagcaggt tgtcctgcgc ggcctcgcgg ccttggcgcg
     7381 ccttctcgga gtcgtagttc tcgccctccc agaagccgcc ggtctcgtcg atgacgcctt
     7441 cctggtcggc gttgaaccag gtgaacgcgc cgtactcgtg ctcgacgccc tcggccttca
     7501 gcttctccca gttgttgtcg tacacgagga acttataggg gctgccgccc tccttgatgt
     7561 cgctttcctt gagcagcgcg gttttcacgt cccagtagga accgtcggtg cgcacggtgg
     7621 ggaagccgga gggctccatg tcctcgcaca cgaggaacgg ggcgttcgtc cacttcttca
     7681 cgtacagctc gtcgtagagc ttcttctcga tgatgacgtt cgtccaggcc agggccaggg
     7741 cgccgtcggt gccggggcgc aggtgctgcc agtagtcggc ttccttgccc atgttggcca
     7801 tgcgggggtc gacgctgatg tgcacgtcgg cgcgcgatgc cacgtccacc gtggtgcggc
     7861 aggagtcgtc gtagttggac agctcggacg cgccgcccca ctgcacgtac actttcgggc
     7921 gcgtgatggt ttccatccac gacatggcga actgcgacac catggtggtg gcgaagtggc
     7981 gcgggccctt gcagatctgc caggcctcaa cgttgttcgg cgtttccagc atgttcttca
     8041 ggatggactc cgagtgcatg caccagatgc gcgacgtgcc cacctggcag gcgatggcct
     8101 cgccgccgtg cttcgccttg atgtccatga agttgtccac gatggactgc atggcctcgt
     8161 cccacgagat gcgctgccag ccgggctcct cgcccttcgg gttcgtgcgc ttcatgggat
     8221 agtgcagacg gtcggggtgg tacgccgcct ggatggacga ctgcgacttc gtgcagcagt
     8281 tgcccatgga ctggaacgct ccctcgtcgc cctcgacgcg tatggcccgc ccgttctgga
     8341 cgatgacctt gacgccgcac tccatcttgc cgcagccgcg gcagcacgtt ttcacggcca
     8401 cggtgtcgtt gccggacacg ctgctgtacg tctcttcggc cagcgcggtg tgcgtcgaag
     8461 cgccgaacgc cgcggctgcc gcaccggtga tggcggcggt cttgacgaac gtgcgtcgtg
     8521 acatggtcag gttacccatg tttgtcctcc ttattagtta atcataatac aaacagacca
     8581 gattgtctgt ttgttactac atttattgta caacacgagc ccatttttgt caaataaaat
     8641 ttaaattata tcaacgttaa taagacgttg tcaataaaat tattttgaca aaatagaata
     8701 gtaatacagg atccgaatcg tttcagttgc ctaaataata aaaaagccgg attaataatc
     8761 aaataaccgt tccttcaatg ttttttttag aaaatttagt tataatatta gatatgatac
     8821 ttttaaatat ctaattcaag cttcaaaaaa caccaactta gttcggtgga taaacaaagg
     8881 agtggttatt attcaaattg cagatcaagc tttagtaaaa aaaatggtga tcatgagtcc
     8941 aaagagaaga acacaggcag agcgcgcaat ggagacccag ggcaagttga ttgcagcggc
     9001 cctgggggtt ttacgggaaa aaggttacgc gggattccgg atcgcagatg tgcccggtgc
     9061 tgctggtgtc tccagaggag cgcagagcca tcatttcccg acaaagcttg agcttctgct
     9121 tgccactttt gaatggcttt acgaacagat caccgaacgc agtcgggctc gattagcgaa
     9181 attgaagcca gaggatgacg tcatccagca aatgctggac gacgccgccg aatttttcct
     9241 cgacgatgac ttctctatca gccttgattt gattgtggct gccgaccggg acccagcgtt
     9301 acgcgagggt attcagcgca cggtagagag gaatcggttt gtcgtcgagg atatgtggct
     9361 tggtgttctg gtgagccgtg gtctttcgcg tgatgatgca gaagatatcc tttggttgat
     9421 attcaattcg gtgcgtgggc ttgctgttcg tagcctatgg cagaaggaca aagaacgctt
     9481 tgagcgtgtc aggaactcga cactcgaaat tgcgcgagag cggtacgcga aattcaagcg
     9541 ctagatctag aagcggccgc gaattcagaa tagtaataca ggatccgaat cgtttcagtt
     9601 gccgccggat taataatcaa ataaccgttc ctaaaaaatg ccctcttggg ttatcaagag
     9661 ggtcattata tttcgcaaaa aaccccgctt cggcggggtt ttttcgcctt gaagacgaaa
     9721 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     9781 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9841 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9901 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9961 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    10021 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    10081 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    10141 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
    10201 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    10261 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    10321 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    10381 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    10441 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    10501 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10561 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10621 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10681 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10741 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10801 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10861 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10921 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10981 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11041 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    11101 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    11161 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    11221 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    11281 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    11341 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11401 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11461 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11521 gagcctatgg aaaaacgcca gcaacgcggc ccgagatgcg ccgcgtgcgg ctgctggaga
    11581 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
    11641 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcatg
    11701 agctcctcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
    11761 tcgactgagc ctttcgtttt atttgaaaaa gaaaagcccc agcaagcttg gcggcagca
//
