LOCUS       sequence-375979-        5730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cng-3 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY75
FEATURES             Location/Qualifiers
     source          1..5730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1095..1115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1137..1157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3321..3343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3443..3462
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3540..3558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3626..3730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3731..3799,3800..4591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3949..3968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4762..5350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5251..5270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5504..5521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5638..5659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5674..5679,5680..5697,5698..5704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5712..5728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5717..5730,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga cgccagaaga
      181 ttcccacaga caacgggagt ttgagtaaat ttaaaactga tgtttcttct acaatctcct
      241 ccttatatag ccatctgata tgacctttca cgtaaaggtc gcaaaggttg tttcacgctt
      301 tattgatagt tcatttgtat gtattctttc gatcataaag ttcctttgtt attgtagaag
      361 tgtggccatg ttgaagttgc ttgcagatta gtttcatgag ttgtgttgca tggtggagtg
      421 tgccgagtgt cataagccgt tgatatagtt ttttaaaaat atttttgaca gtagttagcc
      481 gagaacagga gaggttcatc ctatccgtat ttgcgccaac aatgaaagca ttgtgatgat
      541 ttattaagaa aaaagcaaat ttagatacag tttttgatat atccgttttg ttcaatatgt
      601 ttgccaattt acatatgaac ataaatcgta aataaatatt ttttctttcc tactaacata
      661 atcaaaatga ttctattcat atttttattt tcttcatatc aaatcctttt cactaatacc
      721 ttttcaatcc atggctcaac tcatcggctc atttctctca tttctccaca tgcacctgcg
      781 tcaccaaata taatcactcg tgtattttcc aaaatcatct tcgtacctat ttcctttttc
      841 aacccaatag ttgagtgttt tcttgataac cttcattaat tttaagaaga ctatctaatt
      901 ttaggaagaa tatatagaaa atatacggat tccaatggct tctttaatct aaataaggct
      961 ttattcttgg gagggagaaa cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     1021 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag
     1081 aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa
     1141 aaaagaaacg taaggtcccg cggatgtcag agcttattaa ggagaatatg catatgaaac
     1201 tgtacatgga aggaaccgtg gacaaccatc actttaagtg cacttcggag ggggaaggca
     1261 aaccgtacga gggcacccag accatgcgaa tcaaggtggt cgagggaggc ccactgccct
     1321 tcgccttcga cattctggta agtttaaaca tatatatact aactaaccct gattatttaa
     1381 attttcaggc tactagcttc ctgtacggca gcaagacttt tattaatcac acccagggca
     1441 tcccggactt cttcaaacag tcgttccctg agggcttcac atgggagcgt gtgaccacat
     1501 acgaagacgg tggcgttctc accgctaccc aggacaccag cctccaggac ggttgcctca
     1561 tatataatgt ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1621 agaagatccg gggagtaaac ttcacatcca atggtccggt tatgcagaag aaaacactcg
     1681 gctgggaggc cttcaccgag acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg
     1741 acatggccct gaagctcgtc ggcggtagcc atctcatcgc aaacgctaag accacatata
     1801 gatccaagaa accggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1861 ttcaggctaa gaaccttaaa atgccgggag tctactatgt tgactacaga ctggaacgga
     1921 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggttgcagtt gccagatact
     1981 gcgacctccc gagcaaactg ggtcacaagc ttaatcctag gataccggtg ggtgaagacc
     2041 caccaaagcc atctgccaag ggagccaaga aggccgccaa gaccgttacg aagccaaagg
     2101 acggaaagaa gagacgtcat gcccgtaagg aatcatactc cgtctacatc taccgtgtcc
     2161 tcaagcaagt tcatccagac actggagttt cctccaaagc catgtctatc atgaactctt
     2221 ttgtcaacga tgtcttcgag cgtattgctg ctgaagcatc ccgtcttgct cactacaaca
     2281 agcgttccac aatctcatcc cgcgaaattc agaccgctgt ccgtctgatc cttccaggag
     2341 agcttgccaa gcacgccgtg tctgagggaa ccaaggccgt taccaagtac acttccagca
     2401 agtaggaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     2461 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     2521 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     2581 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     2641 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     2701 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     2761 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     2821 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     2881 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     2941 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     3001 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     3061 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     3121 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     3181 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     3241 atataccaaa cataactgtt tcctactagt cggccgtacg ggccctttcg tctcgcgcgt
     3301 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3361 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3421 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     3481 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg
     3541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4561 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5461 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5521 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5581 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5641 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5701 gttgtgtgga attgtgagcg gataacaatt
//
