LOCUS       sequence-376008-        8664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY72
FEATURES             Location/Qualifiers
     source          1..8664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4023..4043
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4065..4085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6255..6277)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6377..6396
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6474..6492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6560..6664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6665..6733,6734..7525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6883..6902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7696..8284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8185..8204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8438..8455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8572..8593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8608..8613,8614..8631,8632..8638)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8646..8662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8651..8664,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tcactctttc
      181 cgcttcgaaa agtcgtgtgc ttcttcttct tcttgctccg ccgctgctcc gaattggtta
      241 cggaaacttg aactttcttc gatgttttct tcttcgtcgt agtcttccca gtgacgtcgt
      301 cttcttttct ttttcttatt gcttgtgctg ctcaccacca aaatcattgc aaattcaatt
      361 gagaactcgc ggcggcgatg tctccaagtg ttcgaaacag acaaaaaact aaataaaaga
      421 aaaagaagaa gcatgacatg ttgcctttaa attcttcctc aatttttctt caaacttcca
      481 cccccacagt ggcaaaaaac cacatttgct ccgtggccgc gtgacaaaaa ttgatggctg
      541 agttttcatc tctcgcggcc acgtaggata ctagaaagaa gagtgccctc tagaaaactc
      601 ggccacgggt gcgcggaatt tcaaatttcc tctgggtttt tgaagattcc gagcaacttt
      661 cgttttagtc taggtccgag aaaattcgcg gccgccaatt ttttacggcc atgtaggaaa
      721 aaattcattt taggagcatt gaatgatatt gtaggtccta ccgaatttct aaagcgatta
      781 ccgtacccgt ttaaaggcgc atgccacttt tcaccaccct tcacgaccta taaatttatt
      841 ttcgatagtt gcaaaaaaag cgaagaaaac aagaaacgct tgacaaaaca tttccgcagt
      901 tgcaaatcct ttcttaaaac ttgtttgatt ggctcaatcc gttgctcttt tgctcatcct
      961 cctctctttc ccatacacac acatcgaaat atgaatcttc tcttaaaaag aaggttgtcc
     1021 aattagtttc ccctattcat ttttccatgt tttcttataa caactttttc ttttgaatac
     1081 tcctccagaa atgataaatc tttgtgagat gccaattaaa atttattttt gaattttatt
     1141 tgttgaagga ttttatttgt tgaatgacct aaatagaggc tccgcccata tcttggcttt
     1201 ctgcaatttg cataaaaggt tttccagttt tcgtaggtat tttttgaagg tcttccacgt
     1261 aggcgttgcc ggcgtgccta cgcatacctt ctgcatattg ccaaagtact tctgaatcct
     1321 ttcttccaac tatttgattg aatagagaaa accgaggaac tacactaggt aggcaggcag
     1381 gtcgacaggc agtggctacc ttagctgacc agtgaatttt ttttcaaatc gtgttattga
     1441 gacacttgtg cagaagttta aagtgcacag ttttaaaata ttaggcgtct tgttcaaatt
     1501 tgaattttaa tgcctaaata gataggtagg taggtaggca gcacggtagg caagaagtaa
     1561 gataggtagg aaggtatgtt agtaactatt ttataaacta tatacgttat cgaaaggatc
     1621 taaaattttt ctttggggtt caaaaaaggt cctagttttt tgcaataaaa aaaacacaat
     1681 caacgccaac gctctagcta ggtaggcaag ccggtaggta ggcacgcaga taaattggct
     1741 agtaggtagg taggtaggta ggtgggtagg taggtaggta agtaggtata tagctatagg
     1801 taagtgagta agtaagctgt atttttgtgg ttctgagagc ttactacaat tttttctgaa
     1861 aattcgtatt attttttaaa ataaaaaaag ctatcaaccc tcttctaagg tttcaaaaat
     1921 gaaatcattc tcagcgctcc cttttgtaat cagatatcct tcgaaaaaaa gaataccgtc
     1981 aatcttctta aatttcctca aatttcaagc cttttctgga accccggttc caaatttctg
     2041 acggcttctt acaaaagcat atgcacaaat cttttgctcg taaatcatgt gcattcgaaa
     2101 tgatgaggtg ggccctttcg gggtgagacc ggtctcttag cattccgccc ccaatttttc
     2161 caaacttttt ctgccattca ctcaaatatc cattcaattt tcgtttgaaa ataaactttt
     2221 tttttcattc attcaatttc aatttagcat agaacttcga ggagtcattt gttgtataat
     2281 tgaattcctt tatcaagatt agtgaggtgg agcacggggc acgtcatttc agtaatccag
     2341 gtcactgtta cccttatttt tccattaagt gttctgtgcg aattgaaaaa tgttagaatc
     2401 aggaatattg tggccaagat gtttgcggag actagttatc tgaaaacatt tcagtcccta
     2461 gtaaaatgaa gttatcacct tcagaatgtt aggctgatta cttcttcaac ttttgccttt
     2521 ttctaatttt aagctccgcc cacttcttgg ctgttttttt ttgagttaac ctcgcccatt
     2581 tatcacaatg ttgtatattt caactccgcc cacttatcgg gattttttgc atttgtaact
     2641 ccacccgttt cttggtgctt gttttttgca ctgtgagctc ctcccaatta ttgatagttt
     2701 agatttaggc tccgcccctt tctttactgt ttttcgcgtt atgcccagcc acaaatctat
     2761 aattttacag ttttagctcc gcttgaatgc tttcctatca gtaaaattct cggtgttttt
     2821 acattcgatc tctagaattt tcggaaattt tttggtgttg gaaggcgtga cgtcacacga
     2881 gagctagtgt ttagaagaac actaatgacg tcaccctaat aattttcaga atttttttat
     2941 ctaaataaaa atggatccgg tcctaaacta tcttgctagt aattgtttga aaagttgaaa
     3001 attttcagaa ttttaacaaa tttgttgtaa tttcgaacat aaattatgtt gatcgagata
     3061 tcaacttgaa tttttttttt aatacgtaca tgtgcactca acttcaactc taaaaataaa
     3121 aaaacactat ttctatattc tagatctctt tagtgatgta ataataagaa tttccaagct
     3181 atacacctag ccaccgccta ctcacctcat tcagtttccc acgaagaatt agaagaaaat
     3241 gaagaaaaat ggaattgaat agagtcagtg tgagtgtgtg aaatgaaaaa aaaatatata
     3301 tcgatgtggc ggtatctcgg ataaataatt aactatttct cagtttttac ttattctcag
     3361 actctccttt tttgcttttg aatatctctt cttcgtcgtc atcattgaga tttttcttct
     3421 aataatcgta actttgtaat taaaagaaca ttcatttcgg gtcttgcggc gaaaaataca
     3481 tttaaaggtc cacgggttca ttagtatggg tctcactgcg agaaaaagtt tatagtttgt
     3541 atttagtatt cacattgttt ttgaaaataa attgaaatcg ataaaaacta atcaatttga
     3601 aaaaaaatat ttaaaaattc taccacaatt aaatttttaa acttaattta aaaaagaact
     3661 acagcgtaat ttttaaagtt agaactacag agaactgttt ctcgtacact caaaatattg
     3721 aaactgcttt gtgtgtacat ccaaataggc cccgcctttt tctcgttcac tcacggggaa
     3781 aaggcaaaaa tcttgacccc aaccaatatc aggccgccga catcttgcga gttcctctat
     3841 cttatccaat tttcgtctac ttttcttttc gattttcaca tttccaattt taattttcca
     3901 ggacaaatcc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa
     3961 catagaacat tttcaggagg acccttgctt ggagggtacc gagctcagaa aaaatgaccg
     4021 ctccaaagaa gaagcgcaag gttccagtcg agaagatgac tgccccaaaa aagaaacgta
     4081 aggtcccgcg ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     4141 agcgcgctgt gtcaaaagga gaagagctta ttaaggagaa tatgcgttcc aagctctacc
     4201 tcgagggatc cgtcaacgga caccaattca agtgcaccca cgagggagag ggaaagccat
     4261 acgagggaaa gcaaaccaac cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct
     4321 tcgacatcct cgccacccac ttcatgtacg gatccaaggt aagtttaaac atatatatac
     4381 taactaaccc tgattattta aattttcagg tcttcatcaa gtacccagcc gacctcccag
     4441 actacttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcatg gtcttcgagg
     4501 acggaggagt cctcaccgcc acccaagaca cctccctcca agacggagag ctcatctaca
     4561 acgtcaaggt ccgtggagtc aacttcccag ccaacggacc agtcatgcaa aagaagaccc
     4621 tcggatggga gccatccacc gagaccatgt acccagccga cggaggactc gagggacgtt
     4681 gcgacaaggt aagtttaaac atgattttac taactaacta atctgattta aattttcagg
     4741 ccctcaagct cgtcggagga ggacacctcc acgtcaactt caagaccacc tacaagtcca
     4801 agaagccagt caagatgcca ggagtccact acgtcgaccg tcgtctcgag cgtatcaagg
     4861 aggccgacaa cgagacctac gtcgagcaat acgagcacgc cgtcgcccgt tactccaacc
     4921 tcggaggagg aatggacgag ctctacaagc ctaggatacc ggtgggtgaa gacccaccaa
     4981 agccatctgc caagggagcc aagaaggccg ccaagaccgt tacgaagcca aaggacggaa
     5041 agaagagacg tcatgcccgt aaggaatcat actccgtcta catctaccgt gtcctcaagc
     5101 aagttcatcc agacactgga gtttcctcca aagccatgtc tatcatgaac tcttttgtca
     5161 acgatgtctt cgagcgtatt gctgctgaag catcccgtct tgctcactac aacaagcgtt
     5221 ccacaatctc atcccgcgaa attcagaccg ctgtccgtct gatccttcca ggagagcttg
     5281 ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa gtacacttcc agcaagtagg
     5341 aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa aaataatagg
     5401 ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt aatatttaaa
     5461 ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     5521 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     5581 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     5641 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     5701 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     5761 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     5821 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     5881 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     5941 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     6001 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     6061 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     6121 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     6181 caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt
     6241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6361 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6421 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt
     6481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6901 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8521 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8581 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8641 tggaattgtg agcggataac aatt
//
