LOCUS       sequence-376037-        7526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dop-3 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY77
FEATURES             Location/Qualifiers
     source          1..7526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3201..3221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3246..3266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3288..3308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5117..5139)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5239..5258
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5336..5354)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5422..5526
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5527..5595,5596..6387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5745..5764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6558..7146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7047..7066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7434..7455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7470..7475,7476..7493,7494..7500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7508..7524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7513..7526,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga catagagcac
      181 aataatacga gaaagcatac attttccaac tacaatcaaa aaaaaatttc gagcagaaca
      241 cacgcccatt gtgctgcagt aaaaaccgta gttcggaatt ctcaataacc aacacttttt
      301 gcaatgaata cttatgagta ggataatttt agtagtacca cgtactttat atgtcgctta
      361 ttatcaattg aactatacct gaatcaagtt tagtttttta aaataagaag cacaattatc
      421 gtacatacag cttcttgtat tagactatgt tttttcttac ggagttaatc tactagtgtt
      481 ttgttaattt ttaaaagatt attttcttaa aatattgtta ccaatcatgt ggcgttttaa
      541 ttaacaataa acatttttaa acattttcag ttttttttta atgggtactt tttaattaat
      601 tattaaaaac ggcttatcac tcagtttttg ctataattat tctaaaaatc taccaaaaaa
      661 aaatatttaa aaattataat tcgagcttag aagttttaat atagtttcat ttagattttc
      721 caattttttt ttgaaaactc actcagcgat ataccattgt agagggctca gtcctgtagc
      781 aatgcgtaaa gtttttaaaa ttcacagaat ttttaaaatt ttggcaattg gataaaattt
      841 cgtctgaaat tttcaaaaat tctaacaatc ttaaatcaat tattttattc ggtcatgtta
      901 tgttatacct gtttctacgt tttttgcgat cctcaaaatt aagcaaactt atcggaaagt
      961 ttccaaaata tatttccata gagtgagtca gttccacgcg atcaaaacta gattatccga
     1021 attgaaagct ctgacacaca ttttcacacg tgcagttgtg accgtgagag tcattgtgat
     1081 ggaaggtggc ccccttcggc gcacccaaat aaccttatta tcaatcccac agggaacagg
     1141 acgtgtccaa aacctgaccc aaatgacacc actcccccac tcggataaac ttccttattg
     1201 tcctcagcat taacttgtca aaaaatagag tcatgtcttg ttggctgtga atttctaatc
     1261 tttttgcttt tgtttgactt tttgtcttgg aactggtgtg gcgaagcgcc ccatttagtt
     1321 gatcctgtgc cgggattacc atacaacctt ttttgcaata cgctttgcat gagtaccgtc
     1381 tataacccct acaagtctaa tccatcaaat actgagtgta aaaacataga tggcaataaa
     1441 ttgcgcaaaa aatgaaacta gtgaaacttt agctatatgt ctatgtttcc cgctggaata
     1501 tgaaaacatt caatatatta tttgctcttt tgaagaaagt atttcagctc actttcagac
     1561 ttttaaatga aatatatgtt atttgaaaaa acaccgtcga aataccattt aaaacatcca
     1621 gcgacagagg agctgaattt gaactagaaa aatctaattt cgaggatgtg gaattttgca
     1681 agctgtctat aattttgttt tgtgtttaga gtgagaaaaa cgttctggac gctttttgaa
     1741 tttttttgca agtctgaaag ttttcaaatt ttttttgtag aaaataacat tggactgaaa
     1801 acagtttttt ttcatagttc aatttgctca tctgctgctg attttttgta gtgccttatt
     1861 tcaaaagtca tctttccaat aacataggtt tcatttgaaa atataaaaat aagctaaaaa
     1921 ttgagtaatc aaactagcta ctttgtattt tttggtttgt ttagttattt ctattttaac
     1981 tacactttga caatcttgta gcacacctac tcacatattg cgtttttttt ttctgtcacc
     2041 tccaattttt tttttctatt ttctgtgtca ctaccaacat taagtttgct ttttcctatt
     2101 ccggctcttt gttttaacgc catttagagc cgctcttttc tattgtctta aacatttttc
     2161 gcactttcgc tgagattgtt ttgtttcctt cacaccacac gtttaatcgt tatttacatg
     2221 acatgcaaca tttagagttg tttattgttt tgaaactaaa ttcaggcagg ggaccttatg
     2281 aatctaaaaa gtagaatcta gaaaacatag cgtttatagt gaccagacaa ggccaacgca
     2341 caaacttttg atgcataact cggaaaccaa caatgggatg agcgatgatt gttcaacgat
     2401 gcacaaaatc gtagagccat gcaaaattta atggtaaaaa atgatgccac ttttttggcg
     2461 gcggggctca aagctgcaag tgacatcctg aagcagctcg gtagaaaatc agatgttttt
     2521 ggaaattaca tgtctcctca caaggctcat tttgttttgc catttttaag tggtgcttcg
     2581 tatagcatag aaacgtttgt ttttgccggt ccctccatca gcgtgcttta ctcgttcacc
     2641 gacatttttc atatgaagaa catggtgttt tagtgaaact tcgtactttc tcccttctgt
     2701 tttctatttt gctgtgttat ttcttatttc aagaagtggg agacggaacg aagaaaaaac
     2761 gacagggtcc caacagaaaa ctttgacaag aaaccgagcc ctccccagaa agcgtcccat
     2821 cttcccctac accctcttat atatttcttt tcttccccga ataagtctat aaaaagcgat
     2881 gtgggcacct cattcactgg ttttcatatg ccgacaccct cctcaaacac atcagctgcc
     2941 cattttcgca acaacttgtt catagtgctt tccgatactt ccgtattcct tctactaccc
     3001 gtacttatat attattattc ttcttaatat tttctctgga aatccgtcgt ctaaaatcag
     3061 aacccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     3121 acattttcag gaggaccctt ggagggtacc ggttttccca gtcacgacgc gtggtaccga
     3181 gctcagaaaa aatgaccgct ccaaagaaga aacgcaaagt accgagctca gaaaaaatga
     3241 cggcacctaa aaagaaacgc aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc
     3301 gtaaagttcc acgcgtcatg ggagtaagtt taaacagttc ggtactaact aaccatacat
     3361 atttaaattt tcagcgcgct gtctccaagg gagaggagct catcaaggag aacatgcaca
     3421 ccaagctcta catggaggga accgtcaaca accaccactt caagtgcacc cacgaagggg
     3481 agggcaagcc ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc
     3541 tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca
     3601 accacaccca aggaatccca gacttcttca agcaatcctt cgtaagttta aacatgattt
     3661 tactaactaa ctaatctgat ttaaattttc agcctgaggg cttcacatgg gagagagtca
     3721 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
     3781 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
     3841 agaagaaaac actcggctgg gaggccagta ccgagaccct gtaccccgct gacggcggcc
     3901 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
     3961 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgcca ggagtaagtt
     4021 taaacatata tatactaact aaccctgatt atttaaattt tcaggtctac tttgtggacc
     4081 gcagactgga aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg
     4141 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg
     4201 agctgtacaa gtaagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     4261 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     4321 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     4381 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     4441 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     4501 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     4561 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     4621 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     4681 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     4741 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     4801 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     4861 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     4921 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     4981 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     5041 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     5101 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5161 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5221 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5281 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     5341 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5401 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5461 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5521 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5581 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5641 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5701 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5761 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5821 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5881 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5941 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6001 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6061 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6121 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6181 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6241 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6301 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6361 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6421 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6481 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7201 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7261 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgatgaa
     7321 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7501 tgtggaattg tgagcggata acaatt
//
