LOCUS       sequence-376123-        8155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 
            I142V mutant stable expressing cell line under CMVTRE3G promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, 
            Riley NM, Klussendorf M, Zengel JR, Flynn RA, Rustagi A, Widen JC, 
            Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Puschnik AS, 
            Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T, 
            Carette JE
  TITLE     The human disease gene LYSET is essential for lysosomal enzyme 
            transport and viral infection.
  JOURNAL   Science. 2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648.
  PUBMED    36074821
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Sep 8:eabn5648. doi: 10.1126/science.abn5648."
COMMENT     Sequence Label: pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag
FEATURES             Location/Qualifiers
     source          1..8155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3003..3381
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3011..3029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3047..3065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3083..3101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3119..3137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3155..3173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3191..3209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3227..3245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3290..3314
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3819..3842
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3872..4460
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3925..3945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4343..4354)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4474..4973
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4509..4532)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4906..4925
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4994..5593
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4994..5013)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5490..5510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5731..5964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6036..6157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6073..6092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6127..6146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6197..6332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6352..6371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6353..6371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6381..6398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6381..6397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6390..6412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6539..6994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6626..6645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6836..6857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7020..7124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7125..7193,7194..7985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7343..7362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     3001 ttggctttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     3061 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     3121 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     3181 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     3241 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     3301 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     3361 accacttcct accctcgtaa accggttcgt cgactagtcc agtgtggtgg aattctgcag
     3421 ataccatgat gaacttccgt cagcggatgg gatggattgg agtgggattg tatctgttag
     3481 ccagtgcagc agcattttac tatgtttttg aaatcagtga gacttacaac aggctggcct
     3541 tggaacacat tcaacagcac cctgaggagc cccttgaagg aaccacatgg acacactcct
     3601 tgaaagctca attactctcc ttgccttttt gggtgtggac agttattttt ctggtacctt
     3661 acttacagat gtttttgttc ctatactctt gtacaagagc tgatcccaaa acagtgggct
     3721 actgtatcat ccctgtatgc ttggcagtta tttgcaatcg ccaccaggca tttgtcaagg
     3781 cttctaatca gatcagcaga ctacaactga ttgacacgga ttacaaggat gacgacgata
     3841 agtaaatcca gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga
     3901 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3961 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4021 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4081 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4141 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4201 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4261 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4321 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4381 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4441 cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc
     4501 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4561 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4621 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4681 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4741 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4801 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4861 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4921 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     4981 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5041 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5101 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5161 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5221 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5281 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5341 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     5401 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     5461 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5521 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5581 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     5641 ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5701 ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac
     5761 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5821 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5881 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5941 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6001 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6061 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6121 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6301 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6361 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6421 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6481 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6721 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6841 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6901 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6961 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
