LOCUS       sequence-376257-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 
            1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY162
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..21)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(36..662)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     567..586
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        678..733
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    714..732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             751..762
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(769..795,796..798,799..3285,3286..3288,3289..4875)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4939..4960
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      4963..5006
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(5010..5028)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5093..5197
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5198..5266,5267..6058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5416..5435)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6088..6182
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6674..6896
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6944..7894
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     8156..8173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8197..8254)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    8255..8275
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(8382..9095)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             9102..9113
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(9146..9195)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             complement(9398..9409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(10407..10525)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      complement(10583..10610)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        10626..10660
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             10678..11514
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 gccgcgttag cggccttcaa taattggctc tagtattaag acccactttc acatttaagt
       61 tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct
      121 tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt
      181 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
      241 aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
      301 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
      361 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
      421 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
      481 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
      541 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
      601 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa tcttgacatc
      661 attaattcct aatttttgtt gacactctat cgttgataga gttattttac cactccctat
      721 cagtgataga gaaaagaatt gaaaagatct aaagaggaga aaggatctat ggataagaaa
      781 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
      841 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
      901 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgcctc
      961 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     1021 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     1081 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     1141 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     1201 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     1261 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     1321 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     1381 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     1441 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     1501 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     1561 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     1621 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     1681 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     1741 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     1801 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     1861 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     1921 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     1981 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     2041 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     2101 acttttcgta ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     2161 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     2221 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     2281 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     2341 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     2401 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     2461 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     2521 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     2581 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     2641 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     2701 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     2761 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     2821 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     2881 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     2941 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     3001 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     3061 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     3121 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     3181 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     3241 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa
     3301 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     3361 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     3421 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     3481 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     3541 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     3601 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     3661 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     3721 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     3781 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     3841 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     3901 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     3961 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     4021 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga
     4081 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     4141 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     4201 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     4261 gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     4321 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     4381 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     4441 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     4501 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     4561 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     4621 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     4681 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     4741 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     4801 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     4861 ctaggaggtg actaatacta gagccaatta ttgaagggga gcgggaaacc gctccccttt
     4921 ttttgtttct ggtctcccct ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa
     4981 agctaaggat tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa
     5041 tgtcatgata ataatggttt cttagacgtc aggtggcacc atgggaaatg tgcgcggaac
     5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6061 gtcagaccaa gtttacgagc tcgcttggac tcctgttgat agatccagta atgacctcag
     6121 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
     6181 atccaagcac tagggacagt aagacgggta agcctgttga tgataccgct gccttactgg
     6241 gtgcattagc cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa
     6301 tcagagggca ggaactgctg aacagcaaaa agtcagatag caccacatag cagacccgcc
     6361 ataaaacgcc ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag tttccttgca
     6421 tgaatccata aaaggcgcct gtagtgccat ttacccccat tcactgccag agccgtgagc
     6481 gcagcgaact gaatgtcacg aaaaagacag cgactcaggt gcctgatggt cggagacaaa
     6541 aggaatattc agcgatttgc ccgagcttgc gagggtgcta cttaagcctt tagggtttta
     6601 aggtctgttt tgtagaggag caaacagcgt ttgcgacatc cttttgtaat actgcggaac
     6661 tgactaaagt agtgagttat acacagggct gggatctatt ctttttatct ttttttattc
     6721 tttctttatt ctataaatta taaccacttg aatataaaca aaaaaaacac acaaaggtct
     6781 agcggaattt acagagggtc tagcagaatt tacaagtttt ccagcaaagg tctagcagaa
     6841 tttacagata cccacaactc aaaggaaaag gacatgtaat tatcattgac tagcccatct
     6901 caattggtat agtgattaaa atcacctaga ccaattgaga tgtatgtctg aattagttgt
     6961 tttcaaagca aatgaactag cgattagtcg ctatgactta acggagcatg aaaccaagct
     7021 aattttatgc tgtgtggcac tactcaaccc cacgattgaa aaccctacaa ggaaagaacg
     7081 gacggtatcg ttcacttata accaatacgc tcagatgatg aacatcagta gggaaaatgc
     7141 ttatggtgta ttagctaaag caaccagaga gctgatgacg agaactgtgg aaatcaggaa
     7201 tcctttggtt aaaggctttg agattttcca gtggacaaac tatgccaagt tctcaagcga
     7261 aaaattagaa ttagttttta gtgaagagat attgccttat cttttccagt taaaaaaatt
     7321 cataaaatat aatctggaac atgttaagtc ttttgaaaac aaatactcta tgaggattta
     7381 tgagtggtta ttaaaagaac taacacaaaa gaaaactcac aaggcaaata tagagattag
     7441 ccttgatgaa tttaagttca tgttaatgct tgaaaataac taccatgagt ttaaaaggct
     7501 taaccaatgg gttttgaaac caataagtaa agatttaaac acttacagca atatgaaatt
     7561 ggtggttgat aagcgaggcc gcccgactga tacgttgatt ttccaagttg aactagatag
     7621 acaaatggat ctcgtaaccg aacttgagaa caaccagata aaaatgaatg gtgacaaaat
     7681 accaacaacc attacatcag attcctacct acataacgga ctaagaaaaa cactacacga
     7741 tgctttaact gcaaaaattc agctcaccag ttttgaggca aaatttttga gtgacatgca
     7801 aagtaagtat gatctcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact
     7861 agagaacata ctggctaaat acggaaggat ctgaggttct tatggctctt gtatctatca
     7921 gtgaagcatc aagactaaca aacaaaagta gaacaactgt tcaccgttac atatcaaagg
     7981 gaaaactgtc catatgcaca gatgaaaacg gtgtaaaaaa gatagataca tcagagcttt
     8041 tacgagtttt tggtgcattc aaagctgttc accatgaaca gatcgacaat gtaacctcga
     8101 gattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8161 gtcagtgagc gaggaagcct gcataacgcg aagtaatctt ttcggtttta aagaaaaagg
     8221 gcagggtggt gacaccttgc ccttttttgc cggactgcag cggccgctac tagtagggag
     8281 accagaaaca aaaaaaggcc ccccgttagg gaggccttca ataattggct ctagtattat
     8341 taaactgatg cagcgtagtt ttcgtcgttt gcagcaggcc ttttgtacag ttcatccata
     8401 ccatgcgtga tgcccgctgc ggttacgaac tccagcagaa ccatatgatc gcgtttctcg
     8461 ttcggatctt tagacagaac gctttgcgtg ctcagatagt gattgtctgg cagcagaaca
     8521 ggaccatcac cgattggagt gttttgctgg tagtgatcag ccagctgcac gctgccatcc
     8581 tccacgttgt ggcgaatttt aaaattcgct ttaatgccat ttttttgttt atcggcggtg
     8641 atgtaaacat tgtggctgtt aaaattgtat tccagcttat ggcccaggat attgccgtct
     8701 tctttaaagt caatgccttt cagctcaatg cggtttacca gggtatcgcc ttcaaatttc
     8761 acttccgcac gcgttttgta cgtgccgtca tccttaaagg aaatcgtgcg ttcctgcaca
     8821 tagccttccg gcatggcgga cttgaagaag tcatgctgct tcatatggtc cggataacga
     8881 gcaaagcact gaacaccata agtcagcgtc gttaccagag tcggccaagg taccggcagt
     8941 ttaccagtag tacagatgaa cttcagcgtc agtttaccat tagttgcgtc accttcaccc
     9001 tcgccacgca cggaaaactt atgaccgttg acatcaccat ccagttccac cagaataggg
     9061 acgacaccag tgaacagctc ttcgccttta cgcatatggt atttctcctc tttaatctct
     9121 agttaaacaa aattatttgt agaggctgtt tcgtcctcac ggactcatca gaccggaaag
     9181 cacatccggt gacagctctc tagtatttgc gttcaggatt gtcctgctga actgttaata
     9241 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
     9301 gcaacatgcc aggatgagtt agcgaattac actaaccgct gaagtgagga gtcctgggct
     9361 ctagtaggga gaccagaaac aaaaaaaggc cgcgttagcg gccttcaata attggctcta
     9421 gtattaattt gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt
     9481 gcgtgcattc atttgcaaac tgagttgcag caactctttt tcctgctgca tctgaaactc
     9541 acgtaaatcc agcggcagtg ttggaagcga ggtggtttct gaaacggcga tagcgtcttc
     9601 aggcggacgc cgtttaaagg gatcaataat gatgtcatca agcggataat cgctggtgcc
     9661 gtggcgatac actgaacgtt ccaccacgtt tttcaattca cgaatatttc ccggccaacg
     9721 ataattcagc aatgtttctc tggcgcgctc cgtaaacccc gggaacagag gcagcttgat
     9781 ttcccgacac atctggatgg caaagtattc tgccatcaac attatgtcgc tttcgcgctc
     9841 gcgcagtggt ggcagttgta caacatcaaa agccagtcgg tcgagcaggt cagcgcgaaa
     9901 agtgccttca ttgaccatcg ccgggagatc ggcattcgtc gcgcatacca accgcacatt
     9961 cacctgcaat ggttggctgc cgccaacgcg ctccagttca ccgtactcaa tcacgcgcaa
    10021 taatttctcc tgcaccatca tcggtgccgt agcgagttca tcaagaaata gcgtaccgcc
    10081 gtcggcacgt tcaaatctcc ctggatgacg tttttgcgca ccggtaaacg cccccgcttc
    10141 gtgaccaaac agttcggaat ccagcagatt ttcatttaac gccgcgcagt taagggaaat
    10201 aaacggccct tgccaacggg aggagagata atgcaggcgg ctggcaatca gctctttacc
    10261 ggtgccgcgt tcgccgatga tgagcaccgg tttgtccagc ggtgcgagat gcgaaacctg
    10321 ttccagcact tcgagaaagc tgttcgcctc accaagtaaa ttatctttgt attctgccat
    10381 ctagtacttt cctgtgtgac tctagttacg accagtctaa aaagcgcctc aattcgcgac
    10441 cttctcgtta ctgacaggaa aatgggccat tggcaaccag ggaaagatga acgtgatgat
    10501 gttcacaatt tgctgaattg tggtgatgtg atgctcaccg cattcctgct ccagcggcgt
    10561 aaaggtctct ctagtatata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatac
    10621 tagagtttac agctagctca gtcctaggta ttatgctagc tactagaggg agcccaaatg
    10681 accgtattac atagtgtgga tttttttccg tccggtaacg cgtccgtggc gatagaaccc
    10741 cggctcccgc aggcggattt tcctgaacat catcatgatt ttcatgaaat tgtgattgtc
    10801 gaacatggca cgggtattca tgtgtttaat gggcagccct ataccatcac cggtggcacg
    10861 gtctgtttcg tacgcgatca tgatcggcat ctgtatgaac ataccgataa tctgtgtctg
    10921 accaatgtgc tgtatcgctc gccggatcga tttcagtttc tcgccgggct gaatcagttg
    10981 ctgccacaag agctggatgg gcagtatccg tctcactggc gcgttaacca cagcgtattg
    11041 cagcaggtgc gacagctggt tgcacagatg gaacagcagg aaggggaaaa tgatttaccc
    11101 tcgaccgcca gtcgcgagat cttgtttatg caattactgc tcttgctgcg taaaagcagt
    11161 ttgcaggaga acctggaaaa cagcgcatca cgtctcaact tgcttctggc ctggctggag
    11221 gaccattttg ccgatgaggt gaattgggat gccgtggcgg atcaattttc tctttcactg
    11281 cgtacgctac atcggcagct taagcagcaa acgggactga cgcctcagcg atacctgaac
    11341 cgcctgcgac tgatgaaagc ccgacatctg ctacgccaca gcgaggccag cgttactgac
    11401 atcgcctatc gctgtggatt cagcgacagt aaccactttt cgacgctttt tcgccgagag
    11461 tttaactggt caccgcgtga tattcgccag ggacgggatg gctttctgca ataatactag
    11521 ctcggtacca aagacgaaca ataagacgct gaaaagcgtc ttttttcgtt ttggtccctc
    11581 tagtattaat tttgtcttca tgcatgaaga caactaggct ctagtaggga gaccagaaac
    11641 aaaaaaag
//
