LOCUS       sequence-37636-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Mercogliano CP, DeRosier DJ
  TITLE     Concatenated metallothionein as a clonable gold label for electron 
            microscopy.
  JOURNAL   J Struct Biol. 2007 Oct;160(1):70-82. Epub 2007 Jul 10.
  PUBMED    17692533
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol."; date: "2007-10"; volume: "160(1)"; pages: "70-82. Epub 2007 
            Jul 10"
COMMENT     Sequence Label: pMAL-c2x-MT2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..12
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(431..448)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(431..447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(440..462)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(626..643)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      796..882
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcgagggaa ggatttcaag atataccatg gaccccaact gctcctgctc caccggcggc
       61 tcctgcactt gcaccagctc ctgcgcctgc aagaactgca agtgcacctc ctgcaagaag
      121 agctgctgct cctgctgtcc cgtgggctgc tccaaatgtg cccagggctg tgtctgcaaa
      181 ggcgccgcgg acaagtgcac gtgctgtgac atggacccca actgctcctg ctccaccggc
      241 ggctcctgca cttgcaccag ctcctgcgcc tgcaagaact gcaagtgcac ctcctgcaag
      301 aagagctgct gctcctgctg tcccgtgggc tgctccaaat gtgcccaggg ctgtgtctgc
      361 aaaggcgccg cggacaagtg cacgtgctgt gcctaggatc ctctagagtc gacctgcagg
      421 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      481 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      541 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcag cttggctgtt
      601 ttggcggatg agataagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
      661 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
      721 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      781 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctcctg agtangacaa atccgccggg agcgganttg
      901 aacgttgcga acacg
//
