LOCUS       sequence-376584-       10810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heat inducible eGFP reporter with constitutive mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Wu Y, Liu Y, Huang Z, Wang X, Jin Z, Li J, Limsakul P, Zhu L, Allen 
            M, Pan Y, Bussell R, Jacobson A, Liu T, Chien S, Wang Y
  TITLE     Control of the activity of CAR-T cells within tumours via focused 
            ultrasound.
  JOURNAL   Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 
            10.1038/s41551-021-00779-w. Epub 2021 Aug 12.
  PUBMED    34385696
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-021-00779-w"; journalName: "Nat Biomed Eng"; date: 
            "2021-11"; volume: "5"; issue: "11"; pages: "1336-1347"
COMMENT     Sequence Label: pHR-Hsp-eGFP-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..10810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..36
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             105..815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(250..268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     518..537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    892..1480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(945..965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1363..1374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1483..1499)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1484..1500)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1590..1823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1848..1866)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1849..1866)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2156..2175)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2389..2408)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2508..2530
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2568..2586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2654..2758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2759..2827,2828..3619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2977..2996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3790..4378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4279..4298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4532..4549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4619..4639)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4624..4953
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4804..4939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6087..6152
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6282..6302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6727..6861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6764..6783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6818..6837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7029..7662
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7709..7834
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8331..8564
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8749..8793
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9078..9195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(9656..9658,9659..9661,9662..10375)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9656..9658,9659..9661,9662..10372)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9701..9722)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9962..9981)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10309..10330
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(10395..10810,1..84)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10430..10453)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
       61 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatggtg agcaagggcg
      121 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      241 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      301 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      361 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      601 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      781 actccaccgg cggcatggac gagctgtaca agtaaagcgg ccgcgactct agagtcgacc
      841 tgcaggcatg caagcttgca ggcatgcaag cttgatatca agcttatcga taatcaacct
      901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1261 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     1321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     1441 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     1501 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     1561 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     1621 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1801 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     1861 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     1921 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     1981 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     2041 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     2101 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     2161 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     2221 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     2281 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     2341 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     2401 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2461 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2521 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3901 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4261 cacgagggag cttccagggg gaaacgcctg gtatctttat aatcctgtcg ggtttcgcca
     4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4561 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     4621 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4681 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4741 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4861 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4921 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     4981 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     5041 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     5101 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     5161 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     5221 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     5281 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     5341 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     5401 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     5461 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     5521 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     5581 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     5641 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     5701 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     5761 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     5821 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     5881 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     5941 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     6001 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     6061 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     6121 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     6181 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     6241 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     6301 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     6361 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     6421 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     6481 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     6541 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     6601 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     6661 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6721 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6781 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6841 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     6901 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     6961 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     7021 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     7081 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     7141 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     7201 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     7261 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     7321 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     7381 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     7441 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     7501 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7561 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7621 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7681 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7741 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7801 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7861 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7921 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7981 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     8041 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     8101 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     8161 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8221 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8281 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8341 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8401 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8461 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8521 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8581 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8641 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8701 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8761 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8821 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8881 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8941 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     9001 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccaaatg
     9061 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     9121 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     9181 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gatcgataag
     9241 cttgatatcg tcgaggcgcg tcctcagagc cagccgggag gagctagaac cttccccgcg
     9301 tttctttcag cagccctgag tcagaggcgg gctggcctgg catagccgcc cagcctctcg
     9361 gctcacggcc cgatccgccc gaaccttctc ccggggtcag cgccgcgctg cgccgcccgg
     9421 ctgactcagc ccgggcgggc gggcgggagg ctctcgactg ggcgggaagg tgcgggaagg
     9481 ttcgcggcgg cggggtcggg gaggtgcaaa aggatgaaaa gcccgtggaa gcggagctga
     9541 gcagatccga gccgggctgg cggcagagaa accgcaggga gagcctcact gctgagcgcc
     9601 cctcgacggc ggagcggcag cagcctccgt ggcctccagc atccgacaag aagctatggt
     9661 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     9721 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     9781 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     9841 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     9901 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     9961 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
    10021 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
    10081 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
    10141 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
    10201 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
    10261 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
    10321 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataggt
    10381 cgacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
    10441 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
    10501 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
    10561 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
    10621 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
    10681 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
    10741 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
    10801 cgcccgaagg
//
