LOCUS       sequence-376606-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to co-express a mouse Serpinh1 spsgRNA 
            (sg3-Serpinh1) with NLS-mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Sekiguchi R, Mehlferber MM, Matsumoto K, Wang S
  TITLE     Efficient Gene Knockout in Salivary Gland Epithelial Explant 
            Cultures.
  JOURNAL   J Dent Res. 2023 Feb;102(2):197-206. doi: 10.1177/00220345221128201.
            Epub 2022 Nov 10.
  PUBMED    36366748
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1177/00220345221128201"; journalName: "J Dent Res"; date: 
            "2023-02"; volume: "102"; issue: "2"; pages: "197-206"
COMMENT     Sequence Label: 
            pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..230
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     185..205
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             248..428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    475..600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1093..1326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1511..1555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1853..1970
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2021..2269
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2021..2041
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2192..2211
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2291..2366
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2428..2639
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2667..2714
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2715..3407
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2845..2863)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3113..3132
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3417..3473
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3474..3872
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3888..4476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3941..3961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4359..4370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4548..4781
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4807..4824)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4813..5037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5083..5511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5170..5189)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5380..5401
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5520..5540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5525..5854
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5705..5840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5767..5786
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5902..5949
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5968..6342
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6472..6593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6509..6528)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6563..6582
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6642..6658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6642..6658)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6655..6677)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6666..6682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6690..6696,6697..6714,6715..6720))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6735..6756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6873..6890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7044..7632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7124..7143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7803..8594,8595..8663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8426..8445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8664..8768)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8843..8862)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9034..9383,1..30)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
       61 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      121 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      181 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      241 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      301 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      361 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      421 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      481 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      541 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      601 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      661 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      721 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      781 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      841 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      901 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      961 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1021 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1081 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1141 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1201 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1261 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1321 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1381 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1441 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1501 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1561 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1621 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1681 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1741 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1801 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1861 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1921 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1981 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     2041 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     2101 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     2161 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     2221 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg aagttccaag
     2281 gcgaccacac gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     2341 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
     2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 accggttcta gagcgctgcc accatgaagc gacctgccgc cacaaagaag gctggacagg
     2701 ctaagaagaa gaaagtgagc aagggcgagg cagtgatcaa ggagttcatg cggttcaagg
     2761 tgcacatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2821 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     2881 tctcctggga catcctgtcc cctcagttca tgtacggctc cagggccttc atcaagcacc
     2941 ccgccgacat ccccgactac tataagcagt ccttccccga gggcttcaag tgggagcgcg
     3001 tgatgaactt cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg
     3061 gcaccctgat ctacaaggtg aagctccgcg gcaccaactt ccctcctgac ggccccgtaa
     3121 tgcagaagaa gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg
     3181 tgctgaaggg cgacattaag atggccctgc gcctgaagga cggcggccgc tacctggcgg
     3241 acttcaagac cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg
     3301 accgcaagtt ggacatcacc tcccacaacg aggactacac cgtggtggaa cagtacgaac
     3361 gctccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga tccggcgcaa
     3421 caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggcca
     3481 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     3541 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     3601 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     3661 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     3721 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     3781 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     3841 tgctgccctc tggttatgtg tgggagggct aaacgcgtta agtcgacaat caacctctgg
     3901 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3961 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4021 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4081 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4141 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4201 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4261 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4321 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4381 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4441 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     4501 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4561 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4621 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4681 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4741 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     4801 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4861 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4921 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4981 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     5041 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     5101 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5161 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5221 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5281 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5341 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5401 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5461 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     5521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5701 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5761 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5821 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5881 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     5941 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     6001 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     6061 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     6121 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     6181 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     6241 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     6301 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     6361 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6421 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     6481 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6541 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6601 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6661 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6721 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6781 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6841 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6901 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6961 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7021 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7081 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7141 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7201 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7261 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7321 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7381 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7441 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7501 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7561 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7621 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7681 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7741 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7801 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7861 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7921 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7981 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8041 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8101 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8161 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8221 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8281 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8341 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8401 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8461 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8521 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8581 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8641 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8701 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8761 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     8821 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8881 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8941 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     9001 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     9061 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     9121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     9181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     9241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     9301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     9361 gggactttcc tacttggcag tac
//
