LOCUS       sequence-376746-        4230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC43A1_STOP
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1787..1886)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1903..1922)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1904..1922)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1927..1943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1927..1943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2045..2064)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2056..2865
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2123..2142)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3012..3600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3501..3520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3754..3771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3930..3957)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4049..4135)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4184..4206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4198..4215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4199..4215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc acctaccctg
      121 cagcaggcat accggagaag gtggtggatg gcctgtacag ccgtgctgga gaacctgttc
      181 ttttccgccg tgctgctggg atggggatct ctgctgatca tcctgaagaa cgagggcttc
      241 tatagctcca cctgccctgc cgagtctagc acaaatacca cacaggacga gcagcgccgg
      301 tggccaggct gtgaccagca ggatgagatg ctgaatctgg gcttcaccat cggctctttt
      361 gtgctgagcg ccaccacact gccactgggc atcctgatgg acagattcgg accaagacct
      421 gtgaggctgg tgggatccgc ctgctttacc gcctcttgta cactgatggc actggcaagc
      481 agggatgtgg aggccctgtc ccctctgatc ttcctggccc tgtccctgaa cggattcgga
      541 ggcatctgcc tgacctttac atctctgacc ctgccaaaca tgttcggcaa tctgcggtct
      601 acactgatgg ccctgatgat cggcagctac gcctcctctg ccatcacctt tccaggcatc
      661 aagctgatct atgacgccgg cgtggccttc gtggtcatca tgtttacctg gagcggcctg
      721 gcctgcctga tcttcctgaa ctgtacactg aattggccaa tcgaggcatt tccagcacca
      781 gaggaggtga actacaccaa gaagatcaag ctgtccggac tggccctgga ccacaaggtg
      841 accggcgatc tgttctatac acacgtgacc acaatgggac agcgcctgag ccagaaggca
      901 ccatccctgg aggacggctc tgatgccttt atgagccccc aggatgtgcg gggcacctct
      961 gagaatctgc ctgagcgcag cgtgccactg cggaagtccc tgtgctctcc cacattcctg
     1021 tggagcctgc tgaccatggg catgacacag ctgcgcatca tcttttacat ggccgccgtg
     1081 aacaagatgc tggagtatct ggtgaccggc ggccaggagc acgagacaaa tgagcagcag
     1141 cagaaggtgg ccgagaccgt gggcttctac agctccgtgt ttggcgccat gcagctgctg
     1201 tgcctgctga catgtcccct gatcggctat atcatggatt ggagaatcaa ggactgcgtg
     1261 gatgcaccta cccagggaac agtgctgggc gacgcaaggg atggagtggc aaccaagagc
     1321 atcagaccta ggtactgcaa gatccagaag ctgacaaacg ccatctccgc cttcaccctg
     1381 acaaatctgc tgctggtggg ctttggcatc acctgtctga tcaacaatct gcacctgcag
     1441 ttcgtgacct ttgtgctgca cacaatcgtg cggggcttct ttcactccgc ctgcggatct
     1501 ctgtacgcag ccgtgttccc ctccaatcac tttggcaccc tgacaggcct gcagagcctg
     1561 atctccgccg tgttcgcact gctgcagcag cctctgttta tggcaatggt gggacccctg
     1621 aagggagagc ctttctgggt gaacctgggc ctgctgctgt tcagcctgct gggctttctg
     1681 ctgccatcct acctgtttta ctatagggca aggctgcagc aggagtatgc agcaaatggc
     1741 atgggccccc tgaaggtgct gtctggcagc gaggtgaccg cctaagaccc agctttcttg
     1801 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1861 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1921 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1981 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2041 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2101 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2161 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2221 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2281 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2341 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2401 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2461 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2521 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2581 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2641 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2701 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2761 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2821 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2881 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2941 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3841 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3901 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3961 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4021 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4081 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4141 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4201 aaaacgacgg ccagtcttaa gctcgggccc
//
