LOCUS       sequence-376877-        1650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau 
            cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau 
            circRNA 12-->10 K317M with 3X flag tag in exon 10..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda
            Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, 
            de la Grange P, Nelson PT, Stamm S
  TITLE     RNA editing of microtubule-associated protein tau circular RNAs 
            promotes their translation and tau tangle formation.
  JOURNAL   Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 
            10.1093/nar/gkac1129.
  PUBMED    36533443
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-12-9- 9"; volume: "50"; issue: 
            "22"; pages: "12979-12996"
COMMENT     Sequence Label: pcDNA3.1_ZKSCAN1_Tau10-12K317M
FEATURES             Location/Qualifiers
     source          1..1650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             469..534
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gaattcagtg acagtggaga ttgtacagtt ttttcctcga tttgtcagga tttttttttt
       61 ttgacggagt ttaacttctt gtctcccagg taggaagtgc agtggcgtaa tctcggctca
      121 ctacaacctc cacctcctgg gttcaagcgt ttctcctgcc tcagctttcc gagtagctgg
      181 gattacaggc gcctgccacc atgccctgct gacttttgta tttttagtag agacggggtt
      241 tcaccatgtt ggccaggctg gtcttgaact cctgaccgca ggcgattggc ctgcctcggc
      301 ctcccaaagt gctgagatta caggcgtgag ccaccacccc cggcctcagg agcgttctga
      361 tagtgcctcg atgtgctgcc tcctataaag tgttagcagc acagatcact ttttgtaaag
      421 gtacgtacta atgacttttt ttttatactt caggtgcaga taattaatga ctacaaagac
      481 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagaagaag
      541 ctggatctta gcaacgtcca gtccaagtgt ggctcaaagg ataatatcaa acacgtcccg
      601 ggaggcggca gtgtgcaaat agtctacaaa ccagttgacc tgagcatggt gacctccaag
      661 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct
      721 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc
      781 cacgtccctg gcggaggaaa taaaaaggta agaagcaagg tttcatttag gggaagggaa
      841 atgattcagg acgagagtct ttgtgctgct gagtgcctgt gatgaagaag catgttagtc
      901 ctgggcaacg tagcgagacc ccatctctac aaaaaataga aaaattagcc aggtatagtg
      961 gcgcacacct gtgattccag ctacgcagga ggctgaggtg ggaggattgc ttgagcccag
     1021 gaggttgagg ctgcagtgag ctgtaatcat gccactactc caacctgggc aacacagcaa
     1081 ggaccctgtc tcaaaagcta cttacagaaa agaattaggc tcggcacggt agctcacacc
     1141 tgtaatccca gcactttggg aggctgaggc gggcagatca cttgaggtca ggagtttgag
     1201 accagcctgg ccaacatggt gaaaccttgt ctctactaaa aatatgaaaa ttagccaggc
     1261 atggtggcac attcctgtaa tcccagctac tcgggaggct gaggcaggag aatcacttga
     1321 acccaggagg tggaggttgc agtaagccga gatcgtacca ctgtgctcta gccttggtga
     1381 cagagcgaga ctgtcttaaa aaaaaaaaaa aaaaaaaaag aattaattaa aaatttaaaa
     1441 aaaaatgaaa aaaagctgca tgcttgtttt ttgtttttag ttattctaca ttgttgtcat
     1501 tattaccaaa tattggggaa aatacaactt acagaccaat ctcaggagtt aaatgttact
     1561 acgaaggcaa atgaactatg cgtaatgaac ctggtaggca ttagcggccg ctcgagtcta
     1621 gagggcccgt ttaaacccgc tgatcagcct
//
