LOCUS       sequence-376894-        9022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: INTS6
FEATURES             Location/Qualifiers
     source          1..9022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3979..4003)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4128..4169
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4624..4641)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4630..4854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4987..5006)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5138..5185
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5193..6215
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5193..6215
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6345..6466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6382..6401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6436..6455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6515..6531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6515..6531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6528..6550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6539..6555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6563..6569,6570..6587,6588..6593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6608..6629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6746..6763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6917..7505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6997..7016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7676..8467,8468..8536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8299..8318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8537..8641)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8716..8735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatgc
     1321 ccatcttact gttcctgata gacacgtctg cctctatgaa ccagcgcagc catctgggca
     1381 ccacctacct ggacacggcc aaaggcgcgg tagagacctt catgaagctc cgtgcccggg
     1441 accctgccag cagaggagac aggtatatgc tggtcacttt cgaagagccg ccctatgcta
     1501 tcaaggctgg atggaaagaa aaccatgcaa cgtttatgaa tgaattgaaa aaccttcagg
     1561 ctgaaggact tacgactctt ggccaatccc taaggacagc ttttgattta ttaaatttaa
     1621 atagattagt aactggcata gacaactatg ggcagggaag aaaccctttt ttcttggagc
     1681 cagcaataat tatcacaatt actgatggga gcaagttgac taccaccagt ggagtccagg
     1741 atgagcttca tttacctctt aattctcctt tgcctggaag tgaattgacc aaggaacctt
     1801 ttcgttggga tcagagactc tttgcattag tgttgcggtt gcctggcacc atgtcagtag
     1861 aatcagaaca gttgacaggt gtgcctttag atgactctgc aatcacacca atgtgtgaag
     1921 tgacaggcgg ccgttcatat tctgtgtgtt ctccaagaat gcttaatcag tgtctggagt
     1981 ccttggtgca gaaagtacaa agtggggtgg taataaactt tgaaaaagca ggaccagatc
     2041 cttcccctgt agaagatggg cagccagata tatcaaggcc ttttggatct cagccttggc
     2101 atagctgtca caaactcata tatgtcagac caaatcctaa aactggggtt cctataggtc
     2161 attggcctgt tccagagtct ttttggccag atcaaaattc gccaacacta ccacctcgta
     2221 catctcatcc tgtagtgaag ttttcctgta cagactgtga accaatggtt attgataaac
     2281 ttccttttga caaatatgag ttggaacctt caccactgac tcaatttatc ctggaaagga
     2341 aatctcctca aacatgttgg caggtgtacg tgagcaatag tgcaaaatac agtgaacttg
     2401 gtcatccttt tggttacttg aaagccagta cagcactgaa ctgtgtcaac ttatttgtga
     2461 tgccttacaa ttatccagtc cttcttcccc tcttagatga cttgtttaaa gtgcataaag
     2521 caaaaccaac attgaagtgg agacagtcat ttgaaagtta tttgaagaca atgcctccct
     2581 actatcttgg gcccttgaag aaagctgtta ggatgatggg agcacctaac ctaatagcag
     2641 acagtatgga atatggactt agttacagtg tcatttcata cctcaaaaaa ctgagtcaac
     2701 aggccaaaat agaatctgat cgagtcattg gatctgtagg caaaaaagta gtacaggaga
     2761 ctggaataaa agtccggagc cgatcacatg gtttatcaat ggcatatagg aaagattttc
     2821 aacaactcct ccagggaatt tcagaggatg tccctcacag actgctagac cttaatatga
     2881 aggaatacac tgggttccaa gttgctttgc tgaataagga tttgaagcca cagacattta
     2941 gaaatgctta tgacatacca agacgaaatc ttttggatca cttaacaaga atgagatcta
     3001 atcttttgaa gagcactcgc agatttctga aaggacagga cgaagatcaa gtgcacagtg
     3061 ttcctatagc acaaatgggg aactaccagg aatacctcaa gcaagtacct tctccactaa
     3121 gagaacttga tcctgatcag ccacgaaggt tgcatacatt tggcaacccc tttaagctgg
     3181 ataagaaggg tatgatgata gatgaagcag atgaatttgt ggctggacct caaaataaac
     3241 ataaacgacc cggagaacca aatatgcaag ggatccctaa aagacgtcgg tgtatgtctc
     3301 cactactaag aggcagacag cagaatcctg ttgtaaacaa tcatattggg ggaaaaggac
     3361 cacctgcacc tacaactcaa gcacagccag atcttattaa acctcttcct cttcataaaa
     3421 tttcagaaac cactaatgat tcgataatac atgatgtggt tgaaaatcat gttgcagacc
     3481 aactttcatc agacattaca ccaaatgcta tggatacgga attttcagca tcttctccag
     3541 ccagtttact ggaacggcca accaatcata tggaggctct tggtcatgac catttaggaa
     3601 ccaatgacct cactgttggt ggatttttag aaaatcatga ggagccaaga gataaagaac
     3661 aatgtgctga agagaacata ccagcatctt cactcaacaa aggaaagaaa ttgatgcatt
     3721 gcagaagcca tgaagaggtc aatactgaac taaaagcaca aataatgaaa gagatccgaa
     3781 agccaggaag aaaatatgaa agaatcttca ctttactgaa gcatgtgcaa ggcagtttac
     3841 aaacaagact aatattttta caaaatgtca ttaaagaagc atcaaggttt aaaaaacgaa
     3901 tgctaataga acaactggag aacttcttgg atgaaattca tcgaagagcc aatcagatca
     3961 accatattaa tagcaattac ccaactttct tgtacaaagt ggttgatatc cagcacagtg
     4021 gcggccgctc gagtctagag ggcccgcggt tcgaaaccag cggaggcggc ggaagcgggg
     4081 gaggaggcag cggcggaggc ggcagcgggg gcggcggaag cgctaccggt aagcctatcc
     4141 ctaaccctct cctcggtctc gattctacgc gtaccggtat ggcttctaac tttactcagt
     4201 tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc aacttcgcta
     4261 acggggtcgc tgaatggatc agctctaact cgcgatcaca ggcttacaaa gtaacctgta
     4321 gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag gtgcctaaag
     4381 tggcaaccca gactgttggt ggtgaagagc ttcctgtagc cggatggaga tcttacttaa
     4441 atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg
     4501 caatgcaagg tctcctaaaa gatggaaacc cgattccctc ggccatcgcg gccaactccg
     4561 gcatctacgg aggtggaggt ggatctcgta cgggttagta atgagtttaa acccgctgat
     4621 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4681 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4741 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4801 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4861 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4921 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4981 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5041 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5101 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     5161 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     5221 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     5281 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     5341 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     5401 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     5461 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     5521 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     5581 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     5641 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     5701 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5761 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     5821 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     5881 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     5941 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     6001 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     6061 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     6121 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     6181 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     6241 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6301 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6481 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6541 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6601 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6661 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6721 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6781 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6841 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7621 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7681 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7741 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7801 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7861 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7921 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7981 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8041 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8101 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8161 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8221 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8281 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8341 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8401 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8461 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8521 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8581 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8641 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8761 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8821 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8881 cgatgtacgg gccagatata cgcgttgaca ttgattattg actaggcttt tgcaaaaagc
     8941 tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9001 ggtcttgaaa ggagtgggaa tt
//
