LOCUS       sequence-377544-       13583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the Expression of the E3 complex with an FII (F32A, 
            I35A, and I36A) mutation. SMC Internal reference: SMC1728.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13583)
  TITLE     Sascha Martens Lab: ASAP Shared Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 
            pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP
            -TEV-StrepII
FEATURES             Location/Qualifiers
     source          1..13583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1014..1019,1020..1036,1037..1042))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1202..1293
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1352..1381
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1388..1408
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2293..2310
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2454..2588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2760..2851
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5069..5086
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5230..5364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5536..5627
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             6396..6413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6557..6691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6863..6954
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             7483..7500
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7644..7778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7950..8041
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             join(9933..9935,9936..9938,9939..10649)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9978..9999)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10239..10258)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10586..10607
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10662..10682
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10701..10724
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10778..10795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    10939..11073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  11233..11398
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(11669..11688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(11754..12569)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11754..12569)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12477..12496
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12561..12580
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      12691..13279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13180..13199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13433..13450
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
      841 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
      901 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
      961 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     1021 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1081 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1141 gcaaggtttc acctgtttaa acactagtat cgattcgcga cctactccgg aatattaata
     1201 gatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     1261 cgtaacagtt ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc
     1321 atcgggcgac ggatcccggt ccgaaaccat gcaccatcac catcaccatc accatcacca
     1381 tggcggtgaa aacctgtatt tccagggctc cggtatgacg gatgataaag atgttctgag
     1441 agatgtctgg ttcggacgca ttcctacctg cttcacgctg taccaagatg agattacgga
     1501 gagggaggct gaaccctact acctgctgct gccaagagtc agctacctga ctctggtgac
     1561 cgacaaggtc aagaagcact tccagaaggt catgaggcag gaggacatct ctgaaatctg
     1621 gttcgagtac gaaggaactc ctctgaagtg gcactacccc atcggtctgc tgttcgacct
     1681 gctggcttcc agctctgccc tgccttggaa catcaccgtc cacttcaaga gcttcccaga
     1741 gaaggacctg ctgcactgcc cttcaaagga cgctatcgag gcccacttca tgtcctgcat
     1801 gaaggaagct gacgccctga agcacaagtc ccaggtcatc aacgaaatgc agaagaagga
     1861 ccacaagcag ctgtggatgg gtctgcaaaa cgaccgcttc gaccagttct gggctatcaa
     1921 ccgtaagctg atggagtacc ctgccgagga aaacggcttc cgctacatcc ccttccgtat
     1981 ctaccagacc actaccgaaa ggcccttcat ccagaagctg ttcagaccag tggctgccga
     2041 cggtcagctg cacactctgg gcgacctgct gaaggaggtc tgcccatcag ctatcgaccc
     2101 tgaggacgga gaaaagaaga accaggtcat gatccacgga atcgagccaa tgctggaaac
     2161 ccctctgcaa tggctgtccg aacacctctc ctacccagac aacttcctcc acatttccat
     2221 tattccccaa cctacggact aacgccatta acctgatgtt ctggggaata taagcttgcg
     2281 gccgcactcg agcaccacca ccaccaccac tgacatgcgg taccaagctt gtcgagaagt
     2341 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2401 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2581 tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa
     2641 gctgagttgg ctgctgccac cgctgagcaa taactatcat aacccctagg gtatacccat
     2701 ctaattggaa cagtttaaac actagtatcg attcgcgacc tactccggaa tattaataga
     2761 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2821 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
     2881 cgggcgacgg atcccggtcc gaaaccatgg cagcggctac tggtgaccct ggcttgtcta
     2941 aactccaatt cgccccgttc tcctcggcac tcgatgtcgg attctggcat gaactgactc
     3001 agaagaagct gaacgagtac aggctggacg aagctccaaa ggacatcaag ggctactact
     3061 acaacggtga ctccgctggc ctgcctgcca ggctgaccct ggagttctcc gctttcgaca
     3121 tgagcgctcc aactccagcc agatgctgcc cagctatcgg cactctgtac aacaccaaca
     3181 ctctggagag cttcaagact gctgacaaga agctgctgct ggaacaggct gccaacgaaa
     3241 tctgggaatc tatcaagtca ggaaccgccc tggagaaccc agtgctgctg aacaagttcc
     3301 tgctgctgac tttcgctgac ctgaagaagt accacttcta ctactggttc tgctaccctg
     3361 ccctgtgcct gcctgaatcc ctgccactga tccaaggacc tgtcggactg gaccagaggt
     3421 tcagcctgaa gcagatcgag gctctggaat gcgcctacga caacctgtgc cagaccgagg
     3481 gtgtgactgc tctgccatac ttcctgatca agtacgacga aaacatggtg ctggtgtccc
     3541 tgctgaagca ctactcagac ttcttccagg gacagcgtac caagatcact atcggtgtgt
     3601 acgacccttg caacctcgct cagtaccctg gatggccact gcgcaacttc ctggtcctgg
     3661 ctgcccaccg ttggtccagc tctttccagt ccgtggaggt ggtctgcttc agggacagaa
     3721 ccatgcaggg tgctagggac gtcgcccact ccatcatctt cgaggtgaag ctgcctgaaa
     3781 tggctttcag cccagactgc cctaaggccg tcggttggga aaagaaccag aagggtggca
     3841 tgggcccccg catggtgaac ctgtctgagt gcatggaccc aaagcgtctg gctgaatcat
     3901 ccgtggacct gaacctgaag ctgatgtgct ggagactggt ccccactctg gacctggaca
     3961 aggtggtgtc cgtgaagtgc ctgctgctgg gtgctggaac cctgggatgc aacgtcgcca
     4021 ggactctgat gggttggggc gtgagacaca tcaccttcgt ggacaacgcc aagatcagct
     4081 acagcaaccc agtgcgtcag cctctgtacg agttcgagga ctgcctggga ggtggcaagc
     4141 caaaggctct ggctgccgct gacaggctgc aaaagatttt ccctggtgtc aacgccagag
     4201 gattcaacat gtccatcccc atgccaggtc accctgtgaa cttcagctct gtcaccctgg
     4261 agcaggctcg cagggacgtg gagcagctgg aacagctgat cgagagccac gacgtggtct
     4321 tcctgctgat ggacactagg gaatcccgct ggctgcctgc cgtcatcgct gcttcaaagc
     4381 gcaagctggt catcaacgcc gctctgggat tcgacacctt cgtggtcatg cgtcacggtc
     4441 tgaagaagcc caagcagcag ggagctggtg acctgtgccc caaccaccca gtcgcctctg
     4501 ctgacctgct gggttcatcc ctgttcgcta acatccccgg atacaagctg ggttgctact
     4561 tctgcaacga cgtggtggcc ccaggcgact ctacccgcga ccgtactctg gaccagcagt
     4621 gcaccgtgtc aaggcccggt ctggctgtca tcgctggcgc tctggctgtg gagctgatgg
     4681 tgtctgtcct gcaacaccca gaaggaggtt acgctatcgc cagctcttca gacgacagaa
     4741 tgaacgagcc tcccacttca ctgggcctgg tgcctcacca gatccgcgga ttcctgtccc
     4801 gtttcgacaa cgtgctgccc gtcagcctgg ctttcgacaa gtgcaccgcc tgctccagca
     4861 aggtcctgga ccagtacgag cgcgaaggct tcaacttcct ggctaaggtg ttcaactctt
     4921 cacacagctt cctggaagac ctgaccggac tgactctgct ccaccaggaa acgcaggcgg
     4981 ctgagatttg ggatatgagc gacgatgaga ctatttaacg ccattaacct gatgttctgg
     5041 ggaatataag cttgcggccg cactcgagca ccaccaccac caccactgac atgcggtacc
     5101 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     5161 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5221 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5341 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa
     5401 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc
     5461 cctagggtat acccatctaa ttggggctgt ttaaacacta gtatcgattc gcgacctact
     5521 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     5581 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     5641 cataccgtcc caccatcggg cgacggatcc cggtccgaaa ccatggagga ggacgagttc
     5701 atcggagaaa agacgttcca aagatactgt gcggagttca tcaaacattc ccagcaaatc
     5761 ggagatagtt gggagtggcg cccttccaag gactgctcag acggttacat gtgcaagatc
     5821 cacttccaga tcaagaacgg ctctgtgatg tcacacctgg gagcctccac ccacggtcag
     5881 acttgcctgc caatggagga ggctttcgag ctgcctctgg acgactgcga ggtcatcgaa
     5941 accgctgccg cttccgaggt catcaagtac gaataccacg tcctgtactc ctgctcctac
     6001 caggtgcctg tcctgtactt cagggccagc ttcctggacg gccgtcccct gactctgaag
     6061 gacatctggg agggagtgca cgaatgctac aagatgcgcc tgctgcaagg accttgggac
     6121 accatcactc agcaggagca ccctatcctg ggtcagccct tcttcgtgct gcacccctgc
     6181 aagaccaacg agttcatgac tccagtcctg aagaactctc agaagatcaa caagaacgtg
     6241 aactacatca cctcctggct gagcatcgtc ggtcctgtgg tcggcctcaa cctcccattg
     6301 tcctacgcaa aggcgacgag ccaagacgaa agaaacgtgc cctaacgcca ttaacctgat
     6361 gttctgggga atataagctt gcggccgcac tcgagcacca ccaccaccac cactgacatg
     6421 cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     6481 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     6541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6661 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg
     6721 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat
     6781 cataacccct agggtatacc catctaattg gtcaggttta aacactagta tcgattcgcg
     6841 acctactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     6901 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     6961 gattattcat accgtcccac catcgggcga cggatcccgg tccgaaacca tggccgaaga
     7021 gccccagagc gttttgcagt tgcccacaag cattgccgca ggaggcgaag gactcacaga
     7081 tgttagcccc gagactacca ctcctgagcc accttcttca gccgctgtct cacctggtac
     7141 tgaagagcct gctggtgaca ctaagaagaa gatcgacatc ctgctgaagg ctgtcggtga
     7201 cactcctatc atgaagacca agaagtgggc tgtggagagg acccgtacta tccagggtct
     7261 gatcgacttc atcaagaagt tcctgaagct ggtggcttct gaacagctgt tcatctacgt
     7321 gaaccagtcc ttcgctccat ctcctgacca ggaagtgggt actctgtacg agtgtttcgg
     7381 aagcgacggc aagttggttc tccattactg caaatcacaa gcgtggggct aacgccatta
     7441 acctgatgtt ctggggaata taagcttgcg gccgcactcg agcaccacca ccaccaccac
     7501 tgacatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     7561 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     7621 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7681 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7741 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa
     7801 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     7861 taactatcat aacccctagg gtatacccat ctaattggct gcgtttaaac actagtatcg
     7921 attcgcgacc tactccggaa tattaataga tcatggagat aattaaaatg ataaccatct
     7981 cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa
     8041 tattccggat tattcatacc gtcccaccat cgggcgacgg atcccggtcc gaaaccatga
     8101 gcagcggttt gagggcggcg gatttcccca ggtggaagcg tcacatcagc gaacagttgc
     8161 gtagaagaga tagattgcag agacaggctg cggaggaagc ggcgctgcaa tacaacaagc
     8221 tgctggagaa gtctgacctg cactcagtcc tggcccagaa gctgcaagct gaaaagcacg
     8281 acgtgcccaa ccgccacgag atcagccctg gtcacgacgg cacctggaac gacaaccagc
     8341 tgcaagagat ggcccagctg cgcatcaagc accaggagga actgactgaa ctgcacaaga
     8401 agcgtggtga gctggctcag ctggtcatcg acctgaacaa ccagatgcag cgtaaggaca
     8461 gggagatgca gatgaacgaa gctaagatcg ccgagtgcct gcaaaccatc tccgacctgg
     8521 agactgaatg cctggacctg agaaccaagc tgtgcgacct ggaacgcgcc aaccagactc
     8581 tgaaggacga gtacgacgcc ctgcaaatca ccttcactgc tctggaaggc aagctgagga
     8641 agaccactga ggaaaaccag gagctggtga ccaggtggat ggctgagaag gcccaggaag
     8701 ctaacagact gaacgctgag aacgaaaagg actcacgccg taggcaggcc agactgcaaa
     8761 aggagctggc tgaagctgcc aaggaacctc tgcccgtcga gcaggacgac gacatcgaag
     8821 tgatcgtgga cgagacttcc gaccacaccg aggaaacttc cccagtgaga gccatcagcc
     8881 gcgctgctac caagaggctg tcccagcctg ctggtggcct gctggacagc atcactaaca
     8941 tcttcggtag acgctctgtg tccagcttcc cagtccctca ggacaacgtg gacacccacc
     9001 ctggcagcgg aaaggaagtg cgcgtccccg ctactgctct gtgcgtcttc gacgcccacg
     9061 acggagaggt gaacgctgtc cagttctctc ctggttcacg tctgctggcc accggaggta
     9121 tggaccgtag ggtcaagctg tgggaagtgt tcggagagaa gtgcgagttc aagggctcac
     9181 tgtccggaag caacgccggt atcacctcta tcgagttcga ctccgctggt agctacctgc
     9241 tggctgcctc caacgacttc gctagcagga tctggactgt ggacgactac agactgcgcc
     9301 acaccctgac tggacactct ggcaaggtcc tgtcagccaa gttcctgctg gacaacgctc
     9361 gtatcgtgtc tggctcacac gacaggaccc tgaagctgtg ggacctgaga agcaaggtct
     9421 gcatcaagac cgtgttcgcc ggctcttcat gcaacgacat cgtctgcact gaacagtgcg
     9481 tgatgtctgg acacttcgac aagaagatcc gtttctggga catcaggtcc gagagcatcg
     9541 tccgtgagat ggaactgctg ggaaagatca ccgctctgga cctgaaccct gagcgcactg
     9601 aactgctgtc ttgctcacgt gacgacctgc tgaaggtcat cgacctgcgc accaacgcca
     9661 tcaagcagac tttctctgct cccggcttca agtgcggatc agactggacc agagtggtct
     9721 tctccccaga cggtagctac gtcgctgccg gttctgctga aggctcactg tacatctggt
     9781 ctgtgctgac tggcaaggtg gagaaggtcc tgtccaagca gcactccagc tctatcaacg
     9841 ctgtggcctg gtcccccagc ggttcgcatg tggtttcagt ggataagggc tgtaaggcgg
     9901 tgctctgggc tcaatacggc ggcagcggcg gcatggtgag caagggcgag gagctgttca
     9961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
    10021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
    10081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
    10141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
    10201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
    10261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
    10321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
    10381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
    10441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
    10501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
    10561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
    10621 tcactctcgg catggacgag ctgtacaagg gcggcagcgg cgaaaacctg tattttcagg
    10681 gcggcggcag cggctcggcc tggtcgcatc cgcagttcga gaagtaacgc cattaacctg
    10741 atgttctggg gaatataagc ttgcggccgc actcgagcac caccaccacc accactgaca
    10801 tgcggtacca agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
    10861 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    10921 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    11041 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
    11101 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
    11161 atcataaccc ctagggtata cccatctaat tggactaggc tgagtaggca agatgttctg
    11221 gtatgtttaa acaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
    11281 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
    11341 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
    11401 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
    11461 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
    11521 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
    11581 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    11641 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    11701 acgttcgcca aacgtactgc ggtaaagctc atcagcgtgg tcgtgaagaa ttcttagaaa
    11761 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
    11821 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
    11881 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
    11941 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
    12001 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
    12061 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
    12121 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
    12181 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
    12241 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
    12301 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
    12361 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
    12421 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
    12481 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
    12541 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
    12601 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12661 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    12841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12901 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12961 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    13021 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    13081 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13141 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13201 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13261 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    13321 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    13381 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    13441 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    13501 tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata
    13561 aatggatgcc ctgcgtaagc ggg
//
