LOCUS       sequence-377585-       15807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B D433N mutant and His/flag tagged Hs TMEM30A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15807)
  TITLE     transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B and Hs TMEM30A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
FEATURES             Location/Qualifiers
     source          1..15807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             82..262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1790..1809)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2023..2042)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2142..2164
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2202..2220)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2288..2392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2393..2461,2462..3253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2611..2630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3424..4012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3913..3932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4166..4183
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4253..4273)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4258..4587
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4438..4573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5721..5786
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5916..5936
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6361..6495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6398..6417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6452..6471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6663..7296
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7343..7468
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7961..8194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8379..8423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8716..8833
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8892..9195
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9196..9399
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9350..9370
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9396..9420
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9494..9503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10535..10546)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             13892..13945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             13949..13966
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             13967..13990
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14000..14009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    15131..15719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(15184..15204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15602..15613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actggaaggg ctaattcact cccaacgaag acaaaatcgt cgagagatgc tgcatataag
       61 cagctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      121 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      181 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      241 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
      301 cttgcaaaga aatgaatatc agagagtgag aggccttgac attataatag atttagcagg
      361 aattgaacta ggagtggagc acacaggcaa agctgcagaa gtacttggaa gaagccacca
      421 gagatactca cgattctgca catacctggc taatcccaga tcctaaggat tacattaagt
      481 ttactaacat ttatataatg atttatagtt taaagtataa acttatctaa tttactattc
      541 tgacagatat taattaatcc tcaaatatca taagagatga ttactattat ccccatttaa
      601 cacaagagga aactgagagg gaaagatgtt gaagtaattt tcccacaatt acagcatccg
      661 ttagttacga ctctatgatc ttctgacaca aattccattt actcctcacc ctatgactca
      721 gtcgaatata tcaaagttat ggacattatg ctaagtaaca aattaccctt ttatatagta
      781 aatactgagt agattgagag aagaaattgt ttgcaaacct gaatagcttc aagaagaaga
      841 gaagtgagga taagaataac agttgtcatt taacaagttt taacaagtaa cttggttaga
      901 aagggattca aatgcataaa gcaagggata aatttttctg gcaacaagac tatacaatat
      961 aaccttaaat atgacttcaa ataattgttg gaacttgata aaactaatta aatattattg
     1021 aagattatca atattataaa tgtaatttac ttttaaaaag ggaacataga aatgtgtatc
     1081 attagagtag aaaacaatcc ttattatcac aatttgtcaa aacaagtttg ttattaacac
     1141 aagtagaata ctgcattcaa ttaagttgac tgcagatttt gtgttttgtt aaaattagaa
     1201 agagataaca acaatttgaa ttattgaaag taacatgtaa atagttctac atacgttctt
     1261 ttgacatctt gttcaatcat tgatcgaagt tctttatctt ggaagaattt gttccaaaga
     1321 ctctgaaata aggaaaacaa tctattatat agtctcacac ctttgtttta cttttagtga
     1381 tttcaattta ataatgtaaa tggttaaaat ttattcttct ctgagatcat ttcacattgc
     1441 agatagaaaa cctgagactg gggtaatttt tattaaaatc taatttaatc tcagaaacac
     1501 atctttattc taacatcaat ttttccagtt tgatattatc atataaagtc agccttcctc
     1561 atctgcaggt tccacaacaa aaatccaacc aactgtggat caaaaatatt gggaaaaaat
     1621 taaaaatagc aatacaacaa taaaaaaata caaatcagaa aaacagcaca gtataacaac
     1681 tttatttagc atttacaatc tattaggtat tataagtaat ctagccagat cctctacgcc
     1741 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     1801 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     1861 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     1921 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     1981 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     2041 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2101 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2161 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2221 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2281 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2341 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4081 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4141 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4201 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     4261 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4321 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4381 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4441 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4501 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4561 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     4621 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     4681 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     4741 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     4801 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     4861 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     4921 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     4981 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     5041 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     5101 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     5161 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     5221 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     5281 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     5341 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     5401 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     5461 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     5521 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     5581 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     5641 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     5701 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     5761 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     5821 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     5881 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     5941 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     6001 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     6061 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     6121 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     6181 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     6241 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6301 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6481 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     6541 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     6601 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     6661 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     6721 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     6781 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     6841 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6901 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     6961 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     7021 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     7081 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     7141 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7201 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7261 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7321 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7381 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7441 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7501 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7561 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7621 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7681 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7741 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7801 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7861 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7921 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7981 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8041 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8101 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8161 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8221 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8281 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8341 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8401 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8461 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8521 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8581 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8641 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
     8701 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8761 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     8821 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggg gcgataagct
     8881 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     8941 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9001 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9061 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9121 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9181 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     9241 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     9301 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     9361 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     9421 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc
     9481 cgtatacgct agcgccacca tggccctctc agtggactca tcgtggcatc ggtggcagtg
     9541 gagagtcaga gatggcttcc cccattgtcc atcggaaacc acaccgctgc tctctccaga
     9601 gaaagggaga cagagctaca acttgacaca gcagcgggtc gtgttcccca acaacagcat
     9661 attccatcaa gattgggaag aggtctccag gagataccct ggcaacagaa cctgcacaac
     9721 caaatacacc ctcttcacct tcctgccccg gaatctcttt gagcaatttc atagatgggc
     9781 taacctctat ttcctgttcc tggtgatttt gaactggatg ccctccatgg aagtcttcca
     9841 cagagaaatc accatgttac cattggccat tgtcctgttc gtcatcatga tcaaggatgg
     9901 catggaggac ttcaagagac accgctttga taaagcaata aactgctcca acattcgaat
     9961 ttatgaaaga aaagagcaga cctatgtgca gaagtgctgg aaggatgtgc gcgtgggaga
    10021 cttcatccaa atgaaatgca atgagattgt cccagcagac atactcctcc ttttttcctc
    10081 tgaccccaat gggatatgcc atctggaaac tgccagcttg gatggagaga caaacctcaa
    10141 gcaaagatgt gtcgtgaagg gcttctcaca gcaggaggta cagttcgaac cagagctttt
    10201 ccacaatacc atcgtgtgtg agaaacccaa caaccacctc aacaaattta agggttatat
    10261 ggagcatcct gaccagacca ggactggctt tggctgtgag agtcttctgc ttcgaggctg
    10321 caccatcaga aacaccgaga tggctgttgg cattgtcatc tatgcaggcc atgagacgaa
    10381 agccatgctg aacaacagtg gcccccggta caaacgcagc aagattgagc ggcgcatgaa
    10441 tatagacatc ttcttctgca ttgggatcct catcctcatg tgccttattg gagctgtagg
    10501 tcacagcatc tggaatggga cctttgaaga acaccctccc ttcgatgtgc cagatgccaa
    10561 tggcagcttc cttcccagtg cccttggggg cttctacatg ttcctcacaa tgatcatcct
    10621 gctccaggtg ctgatcccca tctctttgta tgtctccatt gagctggtga agctcgggca
    10681 agtgttcttc ttgagcaatg accttgacct gtatgatgaa gagaccgatt tatccattca
    10741 atgtcgagcc ctcaacatcg cagaggactt gggccagatc cagtacatct tctccaataa
    10801 gacggggacc ctgacagaga acaagatggt gttccgacgt tgcaccatca tgggcagcga
    10861 gtattctcac caagaaaatg ctaagcgact ggagacccca aaggagctgg actcagatgg
    10921 tgaagagtgg acccaatacc aatgcctgtc cttctcggct agatgggccc aggatccagc
    10981 aactatgaga agccaaaaag gtgctcagcc tctgaggagg agccagagtg cccgggtgcc
    11041 catccagggc cactaccggc aaaggtctat ggggcaccgt gaaagctcac agcctcctgt
    11101 ggccttcagc agctccatag aaaaagatgt aactccagat aaaaacctac tgaccaaggt
    11161 tcgagatgct gccctgtggt tggagacctt gtcagacagc agacctgcca aggcttccct
    11221 ctccaccacc tcctccattg ctgatttctt ccttgcctta accatctgca actctgtcat
    11281 ggtgtccaca accaccgagc ccaggcagag ggtcaccatc aaaccctcaa gcaaggctct
    11341 ggggacgtcc ctggagaaga ttcagcagct cttccagaag ttgaagctat tgagcctcag
    11401 ccagtcattc tcatccactg caccctctga cacagacctc ggggagagct taggggccaa
    11461 cgtggccacc acagactcgg atgagagaga tgatgcatct gtgtgcagtg gaggtgactc
    11521 cactgatgac ggtggctaca ggagcagcat gtgggaccag ggcgacatcc tggagtctgg
    11581 gtcaggcact tccttggagg aggcattgga ggccccagcc acagacctgg ccaggcctga
    11641 gttctgttac gaggctgaga gccctgatga ggccgccctg gtgcacgctg cccatgccta
    11701 cagcttcaca ctagtgtccc ggacacctga gcaggtgact gtgcgcctgc cccagggcac
    11761 ctgcctcacc ttcagcctcc tctgcaccct gggctttgac tctgtcagga agagaatgtc
    11821 tgtggttgtg aggcacccac tgactggcga gattgttgtc tacaccaagg gtgctgactc
    11881 ggtcatcatg gacctgctgg aagacccagc ctgcgtacct gacattaata tggaaaagaa
    11941 gctgagaaaa atccgagccc ggacccaaaa gcatctagac ttgtatgcaa gagatggcct
    12001 gcgcacacta tgcattgcca agaaggttgt aagcgaagag gacttccgga gatgggccag
    12061 tttccggcgt gaggctgagg catccctcga caaccgagat gagcttctca tggaaactgc
    12121 acagcatctg gagaatcaac tcaccttact tggagccact gggatcgaag accggctgca
    12181 ggaaggagtt ccagatacga ttgccactct gcgggaggct gggatccagc tctgggtcct
    12241 gactggagat aagcaggaga cagcggtcaa cattgcccat tcctgcagac tgttaaatca
    12301 gaccgacact gtttatacca tcaatacaga gaatcaggag acctgtgaat ccatcctcaa
    12361 ttgtgcattg gaagagctaa agcaatttcg tgaactacag aagccagacc gcaagctctt
    12421 tggattccgc ttaccttcca agacaccatc catcacctca gaagctgtgg ttccagaagc
    12481 tggattggtc atcgatggga agacattgaa tgccatcttc cagggaaagc tagagaagaa
    12541 gtttctggaa ttgacccagt attgtcggtc cgtcctgtgc tgccgctcca cgccactcca
    12601 gaagagtatg atagtcaagc tggtgcgaga caagttgcgc gtcatgaccc tttccatagg
    12661 tgatggagca aatgatgtaa gcatgattca agctgctgat attggaattg gaatatctgg
    12721 acaggaaggc atgcaggctg tcatgtccag cgactttgcc atcacccgct ttaagcatct
    12781 caagaagttg ctgctcgtgc atggccactg gtgttactcg cgcctggcca ggatggtggt
    12841 gtactacctc tacaagaacg tgtgctacgt caacctgctc ttctggtatc agttcttctg
    12901 tggtttctcc agctccacca tgattgatta ctggcagatg atattcttca atctcttctt
    12961 tacctccttg cctcctcttg tctttggagt ccttgacaaa gacatctctg cagaaacact
    13021 cctggcattg cctgagctat acaagagtgg ccagaactct gagtgctata acctgtcgac
    13081 tttctggatt tctatggtgg atgcattcta ccagagcctc atctgtttct ttatccctta
    13141 cctggcctat aagggctctg atatagatgt ctttaccttt gggacaccaa tcaacaccat
    13201 ctccctcacc acaatccttt tgcaccaggc aatggaaatg aagacatgga ccattttcca
    13261 cggagtcgtg ctcctcggca gcttcctgat gtactttctg gtatccctcc tgtacaatgc
    13321 cacctgcgtc atctgcaaca gccccaccaa tccctattgg gtgatggaag gccagctctc
    13381 aaaccccact ttctacctcg tctgctttct cacaccagtt gttgctcttc tcccaagata
    13441 ctttttcctg tctctgcaag gaacttgtgg gaagtctcta atctcaaaag ctcagaaaat
    13501 tgacaaactc cccccagaca aaagaaacct ggaaatccag agttggagaa gcagacagag
    13561 gcctgcccct gtccccgaag tggctcgacc aactcaccac ccagtgtcat ctatcacagg
    13621 acaggacttc agtgccagca ccccaaagag ctctaaccct cccaagagga agcatgtgga
    13681 agagtcagta ctccacgaac agagatgtgg cacggagtgc atgagggatg actcatgctc
    13741 aggggactcc tcagctcaac tctcatccgg ggagcacctg ctgggaccta acaggataat
    13801 ggcctactca agaggacaga ctgatatgtg ccggtgctca aagaggagca gccatcgccg
    13861 atcccagagt tcactgacca tatacctcga ggagggcagg ggaagtcttc taacatgcgg
    13921 ggacgtggag gaaaatcccg gccccatgca ccatcaccat caccatgact acaaggacga
    13981 cgatgacaag cttggatctg ccaccatggc gatgaactat aacgcgaagg atgaagtgga
    14041 cggtgggccc ccgtgtgctc cggggggcac cgcgaagact cggagaccgg ataacacggc
    14101 cttcaaacag caacggctgc cagcttggca gcccatcctt acggctggca cggtgctacc
    14161 tattttcttc atcatcggtc tcatcttcat tcccatcggc attggcatct ttgtcacctc
    14221 caacaacatc cgcgagatcg agattgatta taccggaaca gagccttcca gtccctgtaa
    14281 taaatgttta tctccggatg tgacaccttg cttttgtacc attaacttca cactggaaaa
    14341 gtcatttgag ggcaacgtgt ttatgtatta tggactgtct aatttctatc aaaaccatcg
    14401 tcgttacgtg aaatctcgtg atgatagtca actaaatgga gattctagtg ctttgcttaa
    14461 tcccagtaag gaatgtgaac cttatcgaag aaatgaagac aaaccaattg ctccttgtgg
    14521 agctattgcc aacagcatgt ttaatgatac attagaattg tttctcattg gcaatgattc
    14581 ttatcctata cctatcgctt tgaaaaagaa aggtattgct tggtggacag ataaaaatgt
    14641 gaaattcaga aatccccctg gaggagacaa cctggaagaa cgatttaaag gtacaacaaa
    14701 gcctgtgaac tggcttaaac cagtttacat gctggattct gacccagata ataatggatt
    14761 cataaatgag gattttattg tttggatgcg tactgcagca ttacctactt ttcgcaagtt
    14821 gtatcgtctt atagaaagga aaagtgattt acatccaaca ttaccagctg gccgatactc
    14881 tttgaatgtc acatacaatt accctgtaca ttattttgat ggacgaaaac ggatgatctt
    14941 gagcactatt tcatggatgg gaggaaaaaa tccatttttg gggattgctt acatcgctgt
    15001 tggttccatc tccttccttc tgggagttgt actgctagta attaatcata aatatagaaa
    15061 cagtagtaat acagctgaca ttaccattta aactagtccc cgggaccgcg gcacgcgttg
    15121 aattcccgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    15181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    15241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    15301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    15361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    15421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    15481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
    15541 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    15601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    15661 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    15721 tcgggaattc tgtcgactgg tacctttaag accaatgact tacaaggcag ctgtagatct
    15781 tagccacttt ttaaaagaaa agggggg
//
